Method for enlarging genetic variation range of brassica napus

A Brassica napus, genetic variation technology, applied in the field of biological breeding, can solve the problems of slow progress of genetic stability of hybrid offspring, poor compatibility of hybrid offspring, complicated procedures, etc. Effect

Pending Publication Date: 2019-04-02
SOUTHWEST UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] (1) The existing artificially synthesized rapeseed has the difficulty of hybridization compatibility, and the progress of genetic stabilization of the hybrid offspring (artificially synthesized Brassica napus) is relatively slow; it is difficult to select materials with good properties suitable for oil use for production; Breeding has poor compatibility of hybrid offspring, and it is difficult to obtain a new type of Brassica napus with stable genetics and excellent traits, and the genetic base is relatively narrow
[0005] (2) Existing use of Brassica oleracea and Brassica napus hybrids to introduce genomes from Brassica oleracea and Chinese cabbage respectively, cannot obtain more excellent traits for production
[0006] (3) The existing innovation sources of Brassica napus germplasm resources are not wide enough, the range of genetic variation is small, and it is not easy to screen for new Brassica napus with agronomic traits for production
[0007] (4) At present, the excellent traits of the three species of Brassica oleracea, Brassica napus, and Brassica napus are concentrated, and the creation of new Brassica napus requires two interspecific hybridizations and two interspecific hybrid offspring selections, resulting in hybrid incompatibility Or the offspring of hybrids are sterile, which takes a long time, complicated procedures, and heavy workload

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  • Method for enlarging genetic variation range of brassica napus

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Embodiment 1

[0045] The method for widening the Brassica napus mutant library provided by the embodiments of the present invention comprises the following steps:

[0046]Step 1: Select Brassica napus and cabbage (kale, kale, head cabbage, etc.) with excellent properties as experimental materials, and adjust the sowing date appropriately so that the two flowering stages meet. Select a robust Brassica napus strain as the female parent, remove the flower buds that have already bloomed or are too small, and keep the flower buds that can bloom after 2-3 days for peeling and emasculation, and smear the stigma with a 10% NaCl solution by mass ratio , after the stigma is slightly air-dried, give fresh cabbage pollen. Repeat pollination the next day. Bagging was done immediately after each pollination. After 30 days of pollination, the mature immature embryos will be sterilized with 75% alcohol (30-60s) and 10% NaClO in turn, and inoculated on Ms or 1 / 2Ms medium under aseptic conditions, and the ...

Embodiment 2

[0050] Brassica napus 09K26 and 09D311 were used as female parents, kale 09D427 and 09DK005 were used as male parents, about 1,200 flower buds were pollinated, and 71 immature embryos were obtained. After cultivation, 10 clones were obtained, including 3 true hybrids. Brassica napus 09D454, 09D217 was used as the female parent, and Chinese kale AY169, D229, D230 was used as the male parent. About 3,300 flower buds were pollinated, and about 800 immature embryos were obtained. Finally, 31 clones were obtained by inoculating some of the immature embryos. These clones can be divided into three categories according to the number of pollen grains: A, highly sterile; B, partially sterile (with trace amount of pollen); C, fully fertile (morphological performance similar to the female parent, pseudo-hybrid). Among them, type B can obtain a small amount of shriveled seeds through assisted pollination at the bud stage, and a small amount of clones can be obtained through the rescue of im...

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Abstract

The invention belongs to the technical field of biological breeding, and discloses a method for enlarging a genetic variation range of Brassica napus. The method comprises the following steps: interspecifically hybridizing brassica napus and brassica juncea, pollinating, and then rescuing an immature embryo to obtain a triploid hybrid clone; screening hybrid plants with trace pollen for selfing and embryo rescuing to establish clone population S1; performing the selfing for the clone S1 to obtain S2 population, and performing selfing for S2 to obtain S3; performing selfing for S3 to obtain S4,thus obtaining brassica napus; and if S1 has no plant meeting the requirements, continuously performing the embryo rescuing for S1, and selecting the clone of the similar fertile brassica napus for selfing. According to the method of the invention, the hybrid of the brassica napus and natural brassica napus is utilized to enlarge the variation range of the planting resources of the Brassica napus, the hybrid progeny constructs a recombinant inbred line population or microspore culture to construct a DH population by virtue of continuous selfing, and materials with relatively stable heredity and excellent agricultural traits and quality traitsare screened from the population for genetic breeding.

Description

technical field [0001] The invention belongs to the technical field of biological breeding, in particular to a method for widening the genetic variation range of Brassica napus. Background technique [0002] At present, the commonly used prior art in the industry is as follows: rapeseed is one of the main oil crops in the world, and it is also an important edible oil source, protein feed, industrial raw material and nectar source plant. my country is one of the first countries to cultivate rapeseed, but in the past it was mainly the cultivation of cabbage-type rapeseed. In the 1930s, Brassica napus (AACC) began to be introduced and cultivated from North Korea, Japan and the United Kingdom. Cabbage-type rape has become one of the most important oil crops in my country. With the improvement of people's material living standards and the continuous deepening of rapeseed breeding research, people have put forward higher requirements for rapeseed breeding goals, and breeding high...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 周清元崔翠殷家明李加纳唐章林徐新福卢坤林呐曲存民柴友荣
Owner SOUTHWEST UNIVERSITY
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