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A multiplex immunohistochemical analysis kit for Hodgkin's lymphoma and its using method and application

A Hodgkin's lymphoma and immunohistochemical technology, which is applied in the field of multiple immunohistochemical analysis kits for Hodgkin's lymphoma, can solve problems such as the inability to effectively predict the effective inhibitory effect of immune checkpoint inhibitors on tumor-related tissues. Achieve high accuracy, high detection efficiency, and eliminate interference

Active Publication Date: 2020-06-16
GENE CRAB BIOTECH CO +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In order to solve the problem that the prior art cannot effectively predict whether immune checkpoint inhibitors have effectively inhibited tumor-related tissues, the present invention provides a multiple immunohistochemical analysis kit for Hodgkin's lymphoma. According to the kit The test results can effectively judge the effectiveness of immune checkpoint inhibitors, with high sensitivity and good specificity

Method used

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  • A multiplex immunohistochemical analysis kit for Hodgkin's lymphoma and its using method and application
  • A multiplex immunohistochemical analysis kit for Hodgkin's lymphoma and its using method and application
  • A multiplex immunohistochemical analysis kit for Hodgkin's lymphoma and its using method and application

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Embodiment 1

[0099]Two groups of Hodgkin's lymphoma tumor tissues with complete response (complete response, CR) and stable disease (stable disease, SD) were selected for multiple immunohistochemical detection of immune cells, tumor cells and immune checkpoint molecules according to the following steps analyze. There were 11 cases in the CR group and 12 cases in the SD group; each patient had 2 slices, which were used for two panel analysis respectively. A total of 7 molecules were labeled by the two panels. Panel 1 and the staining sequence are: CD3, CD68, PDL1, CD56, CD30, Panel 2 and the staining sequence are: CD3, PD1, PDL1, LAG3, CD30. The primary antibody, secondary antibody, and fluorescent dye information of Panel 1 and Panel 2 are shown in Table 1:

[0100] Table 1 Primary antibody, secondary antibody, fluorescent dye information of Panel 1 and panel 2

[0101]

[0102] Example operation steps:

[0103] 1. Bake the paraffin sections in a 60°C incubator for 120 minutes;

[...

Embodiment 2

[0137] A multiple immunohistochemical analysis kit for Hodgkin's lymphoma, including monoclonal antibody group, antigen retrieval solution, blocking solution, TBST buffer, horseradish peroxidase-labeled goat anti-mouse IgG, hydrogen peroxide, Fluorophore-labeled tyrosinate and DAPI stains;

[0138] The monoclonal antibody group is: CD3 monoclonal antibody, CD30 monoclonal antibody, CD68 monoclonal antibody, CD56 monoclonal antibody, LAG3 monoclonal antibody, PD1 monoclonal antibody and PDL1PDL1PDL1 monoclonal antibody;

[0139] The types of the fluorescent groups are: 520-FITC, 570-Cy3, 620-Cy3.5, 650-Cy5 and 690-Cy5.5.

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Abstract

The invention provides a multi-immunohistochemical analysis kit for hodgkin lymphoma, a use method and application thereof, wherein the kit, the use method and the application relate to the field of multi-immunohistochemical technology. According to the multi-immunohistochemical analysis kit, a monoclonal antibody set comprises more than two and at most six components selected from: a CD3 monoclonal antibody, a CD30 monoclonal antibody, a CD68 monoclonal antibody, a CD56 monoclonal antibody, an LAG3 monoclonal antibody, a PD1 monoclonal antibody and a PDL1 monoclonal antibody. The multi-immunohistochemical analysis kit according to the invention detects a human or animal in-vitro tissue to which a immunization inspection point inhibitor is applied as a sample, and furthermore is used for determining effectiveness of the immunization inspection point inhibitor to the hodgkin lymphoma. The invention further provides a use method of the multi-immunohistochemical analysis kit, wherein no more than six immunization inspection points can be effectively marked on the same tissue, and no cross reaction exists among the multiple marks.

Description

technical field [0001] The invention relates to the technical field of multiple immunohistochemistry, in particular to a Hodgkin's lymphoma multiple immunohistochemical analysis kit and its use method and application. Background technique [0002] Lymphoma is a malignant tumor originating from the lymphatic hematopoietic system, which can be divided into Hodgkin's lymphoma and non-Hodgkin's lymphoma according to pathology. In 2015, the estimated number of new cases of lymphoma in China was 88,000. Hodgkin lymphoma is divided into two categories based on morphology and immunophenotype: nodular lymphocyte-predominant Hodgkin lymphoma and classical Hodgkin lymphoma (classical hodgkin lymphoma, cHL). Four subtypes were enriched in lymphocytic type, mixed cellularity type, nodular sclerosis type and lymphocytopenic type. According to a pathological classification analysis of 10,002 Chinese lymphoma patients, the proportion of Hodgkin's lymphoma was 8.54%, and cHL accounted for ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/574G01N33/533G01N21/25
CPCG01N21/25G01N33/533G01N33/574G01N33/577
Inventor 张恒辉陈衍辉王雅婷罗红丽熊艳彭波周辉
Owner GENE CRAB BIOTECH CO
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