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Esophageal squamous cell carcinoma microenvironmental cell marker molecular model and application thereof

A technology for esophageal squamous cell carcinoma and mast cells, which is applied in analytical materials, measuring devices, instruments, etc., can solve problems such as poor applicability, lack of molecular markers, and tediousness, and achieves high prediction effect, sensitivity and specificity, and high The effect of predicting the effect

Active Publication Date: 2019-03-29
SHANTOU UNIV MEDICAL COLLEGE
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AI Technical Summary

Problems solved by technology

However, there is currently no simple and effective method other than the international TNM staging that can more accurately predict the prognosis of patients with esophageal squamous cell carcinoma
However, TNM staging is cumbersome to predict the prognosis of patients. It is necessary to accurately evaluate the depth of tumor infiltration, the number of metastatic lymph nodes, and whether the tumor has distant metastasis. Moreover, the formulation of international TNM staging lacks a large number of case data of Chinese esophageal squamous cell carcinoma. Therefore, the applicability is relatively poor in evaluating the prognosis of patients in my country; there is a lack of available molecular markers in predicting the sensitivity of radiotherapy and chemotherapy

Method used

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  • Esophageal squamous cell carcinoma microenvironmental cell marker molecular model and application thereof
  • Esophageal squamous cell carcinoma microenvironmental cell marker molecular model and application thereof
  • Esophageal squamous cell carcinoma microenvironmental cell marker molecular model and application thereof

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Embodiment Construction

[0028] In order to make the purpose, technical solution and advantages of the present invention clearer, in this embodiment, 207 surgical specimen archiving wax blocks of esophageal squamous cell carcinoma patients from 2012 to 2014 were collected. The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0029] The steps of immunohistochemical hypersensitivity two-step method are as follows:

[0030] After the specimens were serially sectioned, dewaxed with xylene, and hydrated with graded alcohol, the expression of the target protein was detected by an ultrasensitive two-step method. The specific method is as follows:

[0031] (1) The slices were repaired with 0.01M citrate repair solution, 3% H 2 o 2 After inactivating endogenous peroxidase and blocking with goat serum, incubate with a-SMA, CD163 and CD117 antibodies overnight at 4°C;

[0032] (2) After the slices were incubated with Polymer enhancer and Polymer pol...

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Abstract

The invention relates to an esophageal squamous cell carcinoma microenvironmental cell marker molecular model and application thereof. The molecular model mainly comprises a-SMA+ (invasive frontier) fibroblasts, CD163+ (interstitial) macrophages, CD117+ (protogenic layer) mastocytes and CD117+ (invasion frontier) mastocytes. The molecular model can be used to predict the prognosis of patients withesophageal squamous carcinoma and group adjuvant treatment sensitivity groups. A kit mainly includes an anti-CD117 protein antibody, an anti-CD163 protein antibody and an anti-a-SMA protein antibody.According to the invention, the combination of three molecules combined with histopathological features predicts patients' prognosis, which has a higher predictive effect than single index detectionand clearly distinguishes patients with esophageal squamous carcinoma adjuvant treatment sensitivity; an immune tissue chemical hypersensitivity two-step method on which the molecular model is based is a mature and reliable method which can be widely used in primary hospitals; and compared with international TNM staging, the molecular model has a higher predictive effect, and is simpler, easier, more sensitive and more specific.

Description

technical field [0001] The invention involves combining multiple protein antibodies and histopathological features, and using the immunohistochemical hypersensitive two-step method to detect the expression of esophageal squamous cell carcinoma patient specimens, thereby predicting the prognosis of patients and the sensitivity of adjuvant therapy. Background technique [0002] Esophageal squamous cell carcinoma (ESCC), referred to as esophageal squamous cell carcinoma, is a common malignant tumor of the upper gastrointestinal tract in my country, ranking fourth in the mortality rate of malignant tumors in China. Of the more than 400,000 new cases of esophageal squamous cell carcinoma each year in the world, more than half occur in my country, which is also recognized as the country with the highest incidence of esophageal squamous cell carcinoma in the world. In the 1970s, a nationwide malignant tumor survey was carried out, and Cixian County in Hebei Province, Lin County in ...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/532
CPCG01N33/532G01N33/57484
Inventor 许丽艳何建忠许秀娥吴健谊李恩民
Owner SHANTOU UNIV MEDICAL COLLEGE
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