Schistosoma japonicum infection, and application of components of schistosoma japonicum to prevention and treatment of human tumors
A technology for schistosomiasis and anti-tumor effects, applied in the fields of parasitology and oncology, can solve problems such as unclear mechanisms
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Embodiment 1
[0129] Inhibitory Effect of Schistosoma japonicum Infection on Liver Tumors Induced by DEN in Mice
[0130] C57 mice, male, 5-6 weeks old were used in the experiment; they were divided into PBS group, PBS+infection group (inf), DEN induction group and DEN+infection group.
[0131] DEN induces tumor formation in mice: use 0.25mg / 100ul / mouse, intraperitoneally inject diethylnitrosamine (DEN);
[0132] The mice in the PBS group and the PBS+intersexual infection group were intraperitoneally injected with 100ul PBS as a control;
[0133] Schistosoma infection: 8 weeks after DEN induction, the cercariae of Schistosoma japonicum were infected through the skin of the abdomen, and the cercariae were paired for infection after sex identification, and the infection load was 3-5 pairs per animal.
[0134] Results: The mice were sacrificed at 36 weeks after DEN induction, and the tumor formation in the mice was observed, and the tumor mass, tumor number, tumor size, pathological and molec...
Embodiment 2
[0139] Isolation and Purification of Eggs of Schistosoma japonicum
[0140] Acquisition and infection of cercariae of Schistosoma japonicum: Oncomelania positive for Schistosoma japonicum mainland China strain was provided by Shanghai Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention. The conventional escape method escapes the cercariae of Schistosoma japonicum, and light makes the cercariae float out and gather. The number of cercariae was adjusted in dechlorinated water, and the abdominal hair of New Zealand white rabbits (purchased from Shanghai Slack Experimental Animal Co., Ltd.) was shaved, and the exposed skin was spread, and about 800 Schistosoma japonicum cercariae were infected. Rabbits were reared under conventional feeding conditions.
[0141] Separation and homogenization of New Zealand white rabbit liver: 56 days after cercariae infection, the white rabbit was sacrificed by injecting 5mL of air into the vein of the rabbit's ear, ...
Embodiment 3
[0145] Inhibitory Effect of Schistosoma japonicum Egg Immunization on Subcutaneous Tumor Formation in Mice
[0146] Egg immunization of C57BL / 6 mice: Fresh aseptic Schistosoma japonicum eggs prepared in the above-mentioned Example 2, the concentration was adjusted to 1*10 5 1 / mL, take 50 μL of egg resuspension (that is, the total number of immunized eggs is 5000 eggs), and inject subcutaneously into the dorsal shoulder blade of 6-week-old male C57BL / 6 mice with a 1.2-gauge needle. Before each mouse is injected, it is necessary to lightly flick the syringe to mix the eggs, and hold the needle for about 30 seconds after injection, and then slowly and gently withdraw the needle. At the same time, mice injected subcutaneously with 50 μL blank DMEM medium were used as the control group.
[0147] Inoculation of H22 tumor cells after egg immunization: H22 cells were inoculated intraperitoneally into Kunming mice in advance, and H22 cells were expanded in vivo. After the formation o...
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