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Bacillus tequilensis and application thereof

A technology of Bacillus tequila and Bacillus, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of being blank, and achieve the effects of simple cultivation conditions, easy industrial production, and good development and application prospects

Active Publication Date: 2019-03-29
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the use of biological control methods to screen microbial strains with significant antibacterial effects is almost blank in the research and application of the control of peony blight

Method used

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  • Bacillus tequilensis and application thereof
  • Bacillus tequilensis and application thereof
  • Bacillus tequilensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Screening, Isolation and Purification of Bacillus tequilensis QX13

[0036] 1. Screening and isolation method of strains

[0037] 1. Separation of microorganisms in samples

[0038] (1) In the Peony Cultivation Garden in Yibin District, Luoyang City, the surface soil of peony roots was collected, 50 samples were collected at different locations, and bagged and brought back to the laboratory for air drying. After the air-dried samples were ground, 5g was weighed from each sample and added to a conical flask (250mL) filled with 45mL sterile normal saline and 5 glass beads, and shaken fully for 20-30min to obtain sample suspension.

[0039] (2) Draw 1 mL of the sample suspension under aseptic conditions and add it to a test tube containing 9 mL of sterile saline, shake and mix to prepare 10 -2 Sample diluent (including the dilution factor for preparing the soil sample suspension). Prepare 10 sequentially according to the above method -3 、10 -4 、10 -5 、10 ...

Embodiment 2

[0076] Example 2 Bacillus tequilensis (Bacillus tequilensis) QX13 antibacterial active substance analysis

[0077] Test method: pot test, test time: 5 to 6 months, the specific method is: Bacillus tequilensis (Bacillustequilensis) QX13 is transferred from the inclined test tube of beef extract peptone medium to the triangular flask containing beef extract peptone liquid medium , cultivated on a constant temperature shaker for 20h (180rpm, 30°C), took out the culture solution and centrifuged at 3000r / min for 30min, and collected the supernatant for later use; 8 CFU / mL; bacterial cell suspension, bacterial cell culture fluid (concentration is 10 8 CFU / mL) and the supernatant were evenly sprayed on the peony plants infected by Sclerotinia sclerotinum at 100mL / strain respectively (Sclerotinia sclerotinum was inoculated in potato sucrose liquid medium, cultured at 30°C for 3 days, the mycelium suspension was 100mL Spray peony plants evenly per strain without any treatment 5d). Wa...

Embodiment 3

[0082] (1) Inhibitory effect of Bacillus tequilensis QX13 on white silkworm of peony

[0083] On May 20, 2017, 80 peonies from the Peony Cultivation Garden in Yibin District, Luoyang City were selected as the test subjects, and the peonies were divided into two groups, A and B, and group B was the control group. Inoculate the peony seedlings in the two groups A and B with the same amount of pathogenic fungus Sclerotinia sclerotiorum. The specific method is: inoculate Sclerotinia sclerotinum into potato sucrose liquid medium, culture at 30°C for 3 days, and then add the mycelium suspension to 100 mL Spray peony plants evenly per strain; Group A uses Tequila bacillus (Bacillus tequilensis) QX13 thalline culture solution (concentration is 10) after 5d 8 CFU / mL) 100mL / plant was evenly sprayed on peony plants, sprayed once every two weeks, and sprayed four times in total; Group B was sprayed with the same amount of water, sprayed once every two weeks, sprayed four times in total. ...

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Abstract

The invention belongs to the technical field of biology and particularly relates to Bacillus tequilensis and an application thereof. The Bacillus tequilensis is named Bacillus tequilensis QX13, and the Bacillus tequilensis is collected in CGMCC (China General Microbiological Culture Collection Center) with collection number of CGMCC No.16618 in Beijing China on October 24, 2018. The strain has a remarkable inhibition effect on Sclerotium rolfsii as a pathogenic fungus of peony southern blight, can effectively control peony southern blight and has a remarkable inhibition effect on other plant diseases such as peony brown spot, tomato early blight, Chinese rose shoot blight or pepper phytophthora blight, besides, the strain is harmless to the ecological environment, cannot cause drug resistance of pathogens, is easy to store, facilitates industrial production and has good development and application prospects, and the culture conditions are simple.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a bacillus tequila and an application thereof. Background technique [0002] Peony is a traditional famous flower in my country, which is beautiful and fragrant. Its unique cultural heritage and ornamental value are deeply loved by people. Luoyang has been a famous hometown of peony since ancient times, and it is one of the cultivation centers of peony in China and even in the world. With the continuous expansion of peony planting area, the occurrence of diseases and insect pests is also on the rise, seriously affecting the ornamental value and economic value of peony. Therefore, the problem of pest control of peony has been paid more and more attention by researchers. [0003] There are few pests on peony, but there are many types of diseases and serious damage. Sclerotinia rolfsii is one of the more serious diseases of peony. The pathogenic fungus is Sclerotium rolf...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 宋鹏李捷侯小改赵国栋刘少丹李爱江闫佳吕少洋李民
Owner HENAN UNIV OF SCI & TECH
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