Moulding device, moulding method and application of mouse intervertebral disc degeneration model

Abstract

The invention discloses a moulding device, moulding method and application of a mouse intervertebral disc degeneration model. The moulding device includes one or more moulding barrels and a water tankfor placing the moulding barrel. A position, close to the bottom wall, of the side wall of the moulding barrel or the bottom wall of the moulding barrel is provided with a plurality of water permeating holes, and the water permeating holes communicate with a moulding chamber used for placing mice and located inside the moulding barrel. The moulding device can guide a water avoiding habit of the mice, allows the mice to maintain a bipedal standing posture for a long time, increases the axial stress of the mice, accelerates the process of lumbar intervertebral disc degeneration, forms the intervertebral disc degeneration model, and provides an effective mechanical animal model for the study on the mechanism of mechanical and biological signal transduction of intervertebral disc degeneration.

Description

technical field [0001] The invention relates to the field of intervertebral disc degeneration research, in particular to a modeling device, modeling method and application of a mouse intervertebral disc degeneration model. Background technique [0002] Intervertebral disc degeneration is an important factor leading to low back pain, and animal models are an important basis for studying intervertebral disc degeneration. For a long time, mice, as important experimental animals, have been widely used in the related research of intervertebral disc degeneration. Its advantages are as follows: 1. Low price and easy operation; Fourth, the genome is easy to modify, and can create a variety of genetically modified mice for related research [0003] At present, the intervertebral disc modeling methods for mice mainly fall into the following categories: 1. Mechanical induction models, including pressure models and instability models; 2. Injury models, that is, damage to the annulus fi...

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Problems solved by technology

[0005] 1. In the mechanical induction model, the mechanical compression model requires professional equipment and is poor in operability, and the stress site is the tail vertebra of the mouse, so the simulation is insufficient. On the one hand, the instability model induces intervertebral disc degeneration for a long time, which is not easy On the other hand, fibrosis of the surrounding tissues after surgery may re-stabilize the unstable spine, which is unreliable and has poor repeatability; second, the structural damage model is fast and effective, but its simulation is poor , the transient structural changes caused by trauma account for a large proportion of pathological development; three: natural induction, although the simulation is good, but the cycle is long, the study proves that the intervertebral disc of 18-month-old mice has obvious degenerative changes, which brings Higher cost; 4: Spontaneous model, fast and has a certain degree of simulation but requires high technical level, complicated experimental process and persistent interference factors

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