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Method for simultaneously detecting content of four components in Huangqi Guizhi Wuwu Granule

A technology of five substances and granules of Astragalus and Guizhi, which is applied in the directions of measuring device, material separation, analysis material, etc., can solve the problems of different detection objects and HPLC conditions, unable to detect the four components, etc., and achieves good reproducibility and accuracy. High, fast effects

Inactive Publication Date: 2019-03-26
JIANMIN PHARMA GRP CO LTD
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  • Description
  • Claims
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Problems solved by technology

[0004] At present, the 2015 edition of "Chinese Pharmacopoeia" has recorded the HPLC content determination methods of calycocetin glucoside in astragalus, cinnamic aldehyde in cassia twig, paeoniflorin in peony, and 6-gingerol in ginger, but due to the detection object and HPLC conditions different, resulting in the inability to detect the four components in Huangqi Guizhi Wuwu Granules

Method used

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  • Method for simultaneously detecting content of four components in Huangqi Guizhi Wuwu Granule
  • Method for simultaneously detecting content of four components in Huangqi Guizhi Wuwu Granule
  • Method for simultaneously detecting content of four components in Huangqi Guizhi Wuwu Granule

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Embodiment 1

[0023] The selection of embodiment 1 sample solution preparation method

[0024] In HPLC detection, the preparation of sample solution is mainly based on the polarity, solubility, stability and other properties of the components to be tested to select the appropriate extraction solvent and extraction method, to fully extract the components to be tested from the drug, and at the same time It is possible to remove impurities (including other traditional Chinese medicine ingredients and excipient ingredients) and reduce the impact of impurities on the chromatographic peaks of the components to be measured. Therefore, sample solution preparation has a crucial impact on the sensitivity and accuracy of HPLC detection.

[0025] First, we compared the effects of using water, different concentrations of methanol and ethanol as extraction solvents on the HPLC spectrum, and found that water or ethanol was used as the extraction solvent, there were more impurity peaks in the spectrum, and ...

Embodiment 2

[0034] The selection of embodiment 2 detection wavelength

[0035] Sample solution preparation: 50% methanol (containing 0.5% polysorbate 80) was used as the extraction solvent, and the preparation method was the same as in Example 1.

[0036] Detection wavelength: 200nm, 220nm, 230nm, 260nm, 280nm

[0037] Other conditions are identical with embodiment 1.

[0038] Results: When the wavelength was 200nm, the baseline drifted, and the chromatographic peaks of calycosin glucoside were not completely separated; when the wavelength was 230nm, the response value of each chromatographic peak was reduced, and the peak area decreased compared with that at 220nm. certain influence; when the wavelength is 260nm, the chromatographic peak of 6-gingerol hardly shows; when the wavelength is 280nm, the peak area of ​​the paeoniflorin chromatographic peak of 8.707mim drops sharply, so 220nm is selected as the best detection wavelength.

Embodiment 3

[0039] The selection of embodiment 3 mobile phases

[0040] (1) A is acetonitrile, B is 0.1% phosphoric acid aqueous solution

[0041] (2) A is acetonitrile, B is 0.02% phosphoric acid aqueous solution

[0042] (3) A is acetonitrile, B is 0.3% phosphoric acid aqueous solution

[0043] Detection wavelength: 220nm

[0044] Other conditions are identical with embodiment 1.

[0045] Result: under condition (1), the peak shape and peak separation of four components to be measured are better, and the baseline is stable; under condition (2), each chromatographic peak resolution is unqualified, and the peak shape is also poor; condition (3) When , the separation effect of each chromatographic peak is the same as that of condition (1), but the content of phosphoric acid in the mobile phase is higher, which has a negative impact on the life of the chromatographic column and environmental protection. Therefore, acetonitrile and 0.1% phosphoric acid aqueous solution were selected as t...

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Abstract

The invention discloses a method for simultaneously detecting the content of four components in Huangqi Guizhi Wuwu Granule. The four components are paeoniflorin, calycosin-7-glucoside, cinnamaldehydeand 6-gingerol. The method comprises the steps of extracting a to-be-detected sample by using an extraction solvent: 30-80% methanol, filtering extract and directly detecting by using a high-performance liquid chromatograph. Through the method disclosed by the invention, the content of the four components in the Huangzhi Guizhi Wuwu Granule can be simultaneously detected and the method has the advantages of high efficiency and accuracy and the like, so that the quality of the Huangqi Guizhi Wuwu Granule is more controllable.

Description

technical field [0001] The invention relates to a method for determining the content of Huangqi Guizhi Wuwu granules, belonging to the field of drug analysis. Background technique [0002] Huangqi Guizhi Wuwu Granules is derived from Huangqi Guizhi Wuwu Decoction in "Synopsis of the Golden Chamber". It is made of astragalus, cinnamon sticks, peony, ginger, and jujube. Slight, Cunkou closes slightly, the ruler is small and tight, and the external syndrome is inhumane, such as wind paralysis, Huangqi Guizhi Wuwu Decoction is the main cause." [0003] The quality of medicines is related to the safety of human life. The quality of existing traditional Chinese medicines is mainly controlled by identification or content determination of one or two components. The quality control of some traditional Chinese medicines has great limitations, and the quality of the medicines cannot be fully characterized, resulting in frequent occurrence of adulteration and inferiority of traditional...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 朱立彬陈骞曾庆恢黄志军赵刚翟莉陈鹏李霞
Owner JIANMIN PHARMA GRP CO LTD
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