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Method for increasing acid production rate and extracting rate of glutamic acid

A technology of glutamic acid and acid production rate, which is applied in microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc. problems, to achieve the effect of saving raw material expenses, retaining nutritional value, and reducing high pigment content

Active Publication Date: 2019-03-22
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The optimization of the fermentation medium in the process of preparing glutamic acid by fermentation is an important factor to improve the fermentation efficiency. The applicant's previous patented technology "Method for preparing fermentation medium by using glutamic acid fermentation waste cells" has carried out an improvement on the existing medium Improvement and optimization, including 15% sorghum stalk hydrolyzate, 12% cell hydrolyzate, 5% glucose, 1.2% rice bran extract, 1% corn steep liquor, 0.02% shell powder, 0.02% ferrous sulfate heptahydrate, heptahydrate Magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.01%, and the rest is water; this medium reduces the cost, but there are nitrogen and carbon sources with more pigments, high viscosity and many impurities, making the fermentation process difficult to control and easy to Causes the instability of fermentation and the difficulty of separation and extraction; moreover, the patent document uses high-concentration hydrochloric acid in the treatment method of bacterial protein, which is easy to cause damage to amino acids, resulting in low nutritional value of hydrolyzate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] An amino acid fermentation medium, which includes a fermentation medium A and a fermentation medium B, the fermentation medium A and the fermentation medium B are used separately, and the amino acid is glutamic acid;

[0032] The fermentation medium B is to add inositol and glycerol on the basis of the fermentation medium A;

[0033] The fermentation medium A is: glucose 50g / L, Na 2 HPO 4 12H 2 O 2g / L, KCl 1g / L, MgSO 4 ·7H 2 O 1g / L, MnSO 4 ·H 2 O 3mg / L, FeSO 4 ·7H 2 O 3mg / L, V B1 10mg / L, 1mg / L fulvic acid, 7μg / L biotin, 60ml / L enzymatic hydrolyzate, sterilized at 115°C for 15min;

[0034]The fermentation medium B is: glucose 50g / L, Na 2 HPO 4 12H 2 O 2g / L, KCl 1g / L, MgSO 4 ·7H 2 O 1g / L, MnSO 4 ·H 2 O 3mg / L, FeSO 4 ·7H 2 O 3mg / L, V B1 10mg / L, 1mg / L fulvic acid, 7μg / L biotin, 60ml / L cell enzymolysis solution, 100mg / L inositol, 500mg / L glycerin, sterilized at 115°C for 15min;

[0035] The preparation method of the thallus enzymatic solution is as follow...

Embodiment 2

[0038] A method for improving the acid production rate and extraction rate of glutamic acid, comprising the steps of:

[0039] Brevibacterium flavum GDK-9 (sourced from Tianjin University of Science and Technology, see "Study on variable temperature control process of L-glutamic acid fermentation, Tianjin Chemical Industry Co., Ltd., 2010" for the source of the strain) was inoculated with 12% seed solution (OD 600nm 10) Connect to a 50L automatic fermenter equipped with 30L fermentation medium A for fermentation and cultivation, the fermentation temperature is 38°C, the ventilation ratio is 1:0.7, the stirring speed is 500r / min, and the dissolved oxygen is maintained at 20%. Coupled with a ceramic membrane, when the fermentation lasts to 24 hours, the fermentation liquid in the fermenter is separated through a ceramic membrane (molecular weight cut-off is 20000Da), the filtrate is discharged, the concentrated bacteria are returned to the fermenter, and at the same time, additio...

Embodiment 3

[0041] Influence of hydrolysis process on bacterial proteolysis components:

[0042] Set up a control group,

[0043] Matched group 1: do not adopt high-speed shearing machine to process, all the other are the same as embodiment 1;

[0044] Control group 2: Hydrochloric acid with a concentration of 5 mol / L was used for hydrolysis for 6 hours.

[0045] The wall breaking rate, protein content and total free amino acid content are shown in Table 1:

[0046] group

Example 1

Control group 1

Control group 2

Broken rate%

97.2

83.4

68.7

Protein content mg / g (dried bacteria)

226.1

285.9

268.5

Total free amino acid content mg / g (dried bacteria)

371.5

256.3

187.4

[0047] Visible by table 1, utilize high-speed shearing machine to shear and process 120s, can greatly improve cell wall breaking rate, thereby improve hydrolysis efficiency; The present invention adopts dilute hydrochloric acid to carry out pretreatmen...

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Abstract

The invention belongs to the technical field of amino acids and discloses a method for increasing an acid production rate and an extracting rate of glutamic acid. The method for increasing the acid production rate and the extracting rate of the glutamic acid comprises the following steps: putting a brevibacterium flavum seed solution for producing the glutamic acid into a fermentation tank filledwith fermentation medium to carry out fermentation cultivation; when the brevibacterium flavum seed solution is fermented for 24 hours, separating out a fermentation solution in the fermentation tankvia a ceramic membrane; draining a filter liquor; putting concentrated thalli back into the fermentation tank; and meanwhile, supplementing a fermentation medium B to the fermentation tank; and continuing to carry out fermentation for 16 h to finish fermentation. The method for increasing the acid production rate and the extracting rate of the glutamic acid, disclosed by the invention, has the advantages that the glutamic acid fermentation process is more stable and easy to control by optimizing a culture medium; and moreover, the yield and glucose conversion rate of the glutamic acid are increased, the quality of the fermentation solution is improved, the glutamic acid extracting cost is reduced, and the comprehensive benefits are increased.

Description

technical field [0001] The invention belongs to the technical field of amino acid production and relates to a method for improving the acid production rate and extraction rate of glutamic acid. Background technique [0002] Corynebacterium glutamicum is a strain of glutamic acid fermentation, which belongs to facultative aerobic bacteria. The culture medium composition and culture conditions are different, and the products are also different. In the process of glutamic acid fermentation, when the carbon-nitrogen ratio in the medium changes, it will affect the strain proliferation and glutamic acid synthesis. When the pH of the fermentation broth is acidic, the glutamic acid produced is further converted to acetylglutamine. Therefore, the optimization of glutamic acid fermentation conditions mainly consists of two aspects: medium composition and fermentation process parameter control optimization. During the fermentation process, the fermentation characteristics of the stra...

Claims

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Application Information

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IPC IPC(8): C12P13/14C12N1/20C07C229/24C07C227/40C12R1/13
CPCC07C227/40C12N1/20C12P13/14C07C229/24
Inventor 李德衡赵兰坤徐庆阳马延和刘元涛赵春晓许传高
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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