Tissue culture technology for stephania erecta

A tissue culture and mountain turtle technology, applied in the field of plant tissue culture, can solve problems such as unfavorable protection of high-quality germplasm resources, restricting the promotion and planting of high-quality mountain turtles, and separation of traits of seed reproduction and offspring, and achieves the effect of accelerating popularization and resource development and utilization.

Inactive Publication Date: 2019-03-22
钟天路
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the mountain tortoise mainly reproduces seedlings through seeds, but the separation of offspring traits due to seed propagation is not conducive to the protection of high-quality germplasm resources, and restricts the popularization and planting of high-quality mountain tortoises

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] (1) Induction of clustered buds: Select the budding stems of young trees that grow vigorously and have no diseases and insect pests in the current year as explants, soak them in an aqueous solution of detergent for 6 minutes, then rinse them with tap water for 10 minutes, dry the surface moisture and set aside. Soak in 75% ethanol for 10s in ultra-clean workbench, sterilize with 0.1% mercuric chloride solution for 15min, wash with sterile water for 10 times, dry and cut into 3.0cm-long stems and inoculate them on the induction medium. Cultivate in total darkness at 26°C for 20 days, then place in light for 18 hours a day, light intensity 1000x, and culture temperature 26°C for 40 days to induce the formation of clustered buds, with an induction rate of 70%. The induction medium is MS+3mg / L6-BA+0.7mg / L NAA+0.2mmol / L La(NO 3 ) 2 +3.6% sucrose+0.30% agar+0.08% activated carbon, the pH value is 5.7.

[0013] (2) Proliferation culture: Transfer the clustered buds obtained ...

Embodiment 2

[0017] (1) Induction of clustered buds: Select the budding stems of young trees that grow vigorously and have no diseases and insect pests in the current year as explants, soak them in an aqueous solution of detergent for 6 minutes, then rinse them with tap water for 16 minutes, dry the surface moisture and set aside. Soak in 75% ethanol for 4 seconds in an ultra-clean workbench, sterilize with 0.1% mercuric chloride solution for 10 minutes, wash with sterile water for 4 times, dry and cut into 2.5 cm long stems and inoculate them on the induction medium. Cultivate in total darkness at 26°C for 18 days, then place in light for 14 hours a day, light intensity 1600x, and culture temperature 26°C for 30 days to induce the formation of clustered buds, and the induction rate is 76%. The induction medium is MS+7mg / L6-BA+1.0mg / L NAA+0.5mmol / L La(NO 3 ) 2 +3.5% sucrose+0.40% agar+0.2% activated carbon, the pH value is 5.9.

[0018] (2) Proliferation culture: Transfer the clustered b...

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PUM

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Abstract

The invention discloses a tissue culture technology for stephania erecta. Stephania erecta is selected from dry roots of menispermaceae plants and mainly grows in Yunnan, Guizhou, Guangxi, Sichuan, and the like. Stephania erecta can be harvested throughout the year, optimally in autumn. Stephania erecta is prepared according to the following steps: removing soil and thin fibrous roots, slicing anddrying in the sun. Stephania erecta smells light, tastes bitter, has the functions of clearing away heat and toxic materials, dissolving phlegm, removing stasis and relieving pain, and can be used for treating ulcer pyogenic infection, stomachache, laryngalgia, traumatic injury and venomous snake bites. According to a seedling-growing method for acquiring high-quality seedlings of stephania erecta through an in vitro rapid propagation technology, stephania erecta sections with buds and stems are taken as explants, and then a tissue culture rapid propagation system of stephania erecta is established through the processes of disinfection of explants, induction of multiple shoots, rooting culture, acclimatization and transplanting, and the like. The tissue culture technology has important practical significance in accelerating popularization and resource exploitation and utilization of well-bred stephania erecta.

Description

Technical field [0001] The present invention relates to a method of plant tissue culture in plant biotechnology, and specifically to a tissue culture technology of mountain turtle. Background technique [0002] Mountain turtle is a plant of the family Antipodes, and its dry roots are taken. Mainly produced in Yunnan, Guizhou, Guangxi, Sichuan and other places. It can be harvested all year round, preferably in autumn. Remove soil and fine fibrous roots, slice into slices and dry in the sun. Slight breath and bitter taste. It has the functions of clearing heat, detoxifying, eliminating phlegm, dispersing blood stasis and relieving pain. Used for carbuncle swelling, stomachache, sore throat, bruises, and poisonous snake bites. Mountain turtles have been in short supply for a long time, and man-made predatory logging has led to the depletion of wild mountain turtle resources. Therefore, it is necessary to protect their resources. In recent years, the development of mountai...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 钟天路
Owner 钟天路
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