Method for rapidly identifying stevia rebaudiana leaf spot disease pathogenic bacteria
A technology of stevia leaves and pathogenic bacteria, which is applied in the directions of instruments, hybridization, sequence analysis, etc., can solve the problems affecting the economic output of stevia rebaudiana, agricultural production loss, plant leaf withering, etc., to improve detection efficiency and sensitivity, strong specificity, Simple operation effect
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[0040] A method for rapidly identifying the pathogenic bacteria of stevia rebaudiana leaf spot, the steps are as follows:
[0041] (1) Isolation and purification of pathogenic bacteria
[0042]In July 2017, plants with diseased spots on leaves were collected from the Stevia production base in Fu’an Town, Dongtai City, Jiangsu Province. Disinfect the surface with 70% alcohol for 30 seconds, then treat it with 0.1% mercuric chloride for 2 minutes, and finally rinse it with sterile water for 3 times to remove the moisture on the leaf surface. On the PDA plate culture medium, cultivate in the dark at 25°C for 3 to 5 days, pick a small amount of mycelium from the edge of the newly grown colony and inoculate it on a new PDA plate for further separation and purification, and record the morphological characteristics of the colony;
[0043] Disease samples were collected from the stevia production base in Fu'an Town, Dongtai City, Jiangsu Province. Two strains of anthracnose bacteria ...
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