Method for rapidly identifying stevia rebaudiana leaf spot disease pathogenic bacteria

Abstract

The invention discloses a method for rapidly identifying stevia rebaudiana leaf spot disease pathogenic bacteria and application, and belongs to the technical field of phytopathogen separation and identification. The method for rapidly identifying stevia rebaudiana leaf spot disease pathogenic bacteria comprises the steps that development, evolution analysis, identification and purification of a morphological observation and molecular biological identification combination system are performed, and the species of the stevia rebaudiana leaf spot disease pathogenic bacteria subjected to pathogenicity identification is subjected to molecular biology identification and system development and evolution analysis as ITS, GAPDH and CHS-1 gene segments, and after homologous comparison, system development evolution trees for the ITS, GAPDH and CHS-1 gene segments are constructed respectively. The method for rapidly identifying stevia rebaudiana leaf spot disease pathogenic bacteria is rapid and convenient to implement, and identification errors caused by the inter-species close height are avoided; a foundation is laid for identifying, preventing and breeding the stevia rebaudiana leaf spot disease.

Description

technical field [0001] The invention belongs to the technical field of separation and identification of plant pathogenic bacteria, and in particular relates to a method and application for rapidly identifying the pathogen of stevia leaf spot. Background technique [0002] Stevia [Stevia rebaudiana (Bertoni)] is a perennial herb of the genus Stevia in the family Asteraceae. Stevia leaves are rich in flavonoids and steviol glycoside chemical components. Flavonoids have various pharmacological activities such as anti-tumor and anti-oxidation; steviol glycosides (steviol glycosides) is a new type of natural sweetener, which has the characteristics of high sweetness, low calorie, safety and non-toxicity, etc. Research and development hotspots in the field. In 2017, at the stevia production base in Fuan Town, Dongtai City, Jiangsu Province, it was found that there were different degrees of leaf spots on the leaves of stevia, and all the leaves of the severely affected plants wit...

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Problems solved by technology

In 2017, at the stevia production base in Fu'an Town, Dongtai City, Jiangsu Province, it was found that the leaves of stevia had different degrees of leaf spots, and the leaves of the severely affected plants all withered, which seriously affected the economic output of stevia

In recent years, the diseases of Stevia rebaudiana seedlings in the Hexi Corridor of Gansu Province have been increasing year by year. Damping-off, blight and leaf spot have become the three major diseases that are most likely to occur in the seedling raising stage, causing great losses to agricultural production.

Due to the universal distribution and extreme destructive characteristics of anthracnose bacteria, and there is no report on the biological identification of anthracnose bacteria causing stevia leaf spot in my country, it is urgent to invent a quick and easy method for treating sweet chrysanthemum. Method for accurate identification of Chrysanthemum leaf spot and other pathogens

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