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Halomonas ventosae strain JPT10-1 for improving salt-tolerance capability of corns as well as bacterium agent and application of halomonas ventosae strain JPT10-1

A technology of Halomonas and Fan's salt, applied in the fields of application, bacteria, biocide, etc., can solve the problems of foreign strains not being able to colonize and grow well, weakening of physical and chemical properties, practical application limitations, etc., and achieve stable properties , high number of viable bacteria, and wide application conditions

Active Publication Date: 2019-03-19
SHANDONG AGRICULTURAL UNIVERSITY
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

In recent years, there have been many studies on the salt-tolerant mechanism of plant endophytes, but few endophytes have been screened to help plants resist stress under high salt concentrations.
Moreover, most endophytic bacteria face the following two problems: 1. Exogenous strains cannot colonize well in plants or grow unstable; 2. Some physical and chemical properties of endophytic strains outside the plant (or produce certain Substances) may be weakened or even disappear in plants, which is greatly limited in practical application

Method used

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  • Halomonas ventosae strain JPT10-1 for improving salt-tolerance capability of corns as well as bacterium agent and application of halomonas ventosae strain JPT10-1
  • Halomonas ventosae strain JPT10-1 for improving salt-tolerance capability of corns as well as bacterium agent and application of halomonas ventosae strain JPT10-1
  • Halomonas ventosae strain JPT10-1 for improving salt-tolerance capability of corns as well as bacterium agent and application of halomonas ventosae strain JPT10-1

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Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1: Halomonas fanneri Halomonas ventosae Isolation and purification of JPT10-1

[0032] Add 1 g of soil sample from the Suaeda salsa rhizosphere soil in the Yellow River Estuary into 10 mL of sterile water and mix to make 10 -1 Diluent, pipette 1 ml 10 -1 Add the diluent to 9 ml sterile water and mix well to make 10 -2 diluent, and so on to make 10 -3 、10 -4 、10 -5 Diluent, take 10 -3 、10 -4 、10 -5 The diluted solution was spread on high-salt LB medium (NaCl content 2000 mmol / L), placed in a constant temperature incubator at 28°C for 5 days, and single colonies with different morphological characteristics were selected for purification, and the obtained pure single colonies were prepared as The bacterial suspension was inoculated in corn seedlings, the growth of corn seedlings was observed and its biomass was measured for verification, and the strains that could significantly improve the salt tolerance of corn seedlings were selected, one of which was...

Embodiment 2

[0033] Embodiment 2: Halomonas fanneri Halomonas ventosae Identification of JPT10-1

[0034] 1. Morphological and physiological and biochemical characteristics of the strain

[0035] The colony morphology of bacterial strain JPT10-1 on the high-salt LB solid plate is as follows: figure 1 Shown is round, milky white, opaque, smooth and moist surface, neat edges, producing a small amount of pale yellow pigment. Gram staining of strains in different growth stages (logarithmic phase and stationary phase) was negative, and the morphology was oval under an optical microscope. The growth temperature of the strain JPT10-1 is 18-37°C, the pH is 5.5-8, the salt concentration is 1000-3000 mmol / L, the optimum growth temperature is 28°C, the optimum pH is 7, and the optimum salt concentration is 2000 mmol / L.

[0036] 2. 16S rDNA identification of the strain:

[0037]The genome of strain JPT10-1 was extracted with a genome extraction kit, and using it as a template, 16S rDNA univers...

Embodiment 3

[0040] Embodiment 3: the preparation of bacterial strain JPT10-1 bacterial suspension

[0041] The preparation method of the bacterial suspension of Halomonas fannerii JPT10-1 comprises the following steps: inoculate the purified bacterial strain JPT10-1 into high-salt LB liquid medium according to the inoculum size of 1% (salt concentration is 1000 mmol / L, the rest of the components are the same as LB liquid medium), after culturing in a constant temperature shaking shaker at 28 ℃ 180 rpm for 48 h, take a small amount of bacterial suspension in a 1.5 ml centrifuge tube to measure OD 600 , if it is less than 0.5, continue to cultivate to 0.5-1.0, and store it in a refrigerator at 4 °C. The JPT10-1 bacterial content in the described bacterium suspension that makes is 1 * 10 10 ~3×10 11 pieces / ml.

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Abstract

The invention provides a halomonas ventosae strain JPT10-1 for improving the salt-tolerance capability of corns as well as a bacterium agent and application of the halomonas ventosae strain JPT10-1. The strain is preserved in China Center for Type Culture Collection (CCTCC) on August 17, 2018, with the preservation number of CCTCC M 2018548. The strain can resist high-concentration NaCl and can grow in the environment with the salt concentration of 3000mmol / L, and the growth speed is relatively rapid. The invention further discloses the bacterium agent prepared from the strain and the application of the bacterium agent to the improvement of the salt-tolerance capability of the corns. A bacterium suspension solution of the strain is inoculated into corn seedlings under salt stress, so thatbiomasses including the root length, the plant height and the like, and various physiological indexes including the vitality of a root system, a photosynthetic rate, an anti-oxidization enzyme systemand the like can be remarkably improved. Meanwhile, the vitality of part of a soil enzyme can be changed through the strain, so that the fertility of the soil and the utilization rate of plants on nutrient elements are improved. The strain provided by the invention has important application value on reduction of the stress on the growth of the corns in a saline and alkaline land.

Description

technical field [0001] The invention belongs to the technical field of interaction between microorganisms and plants, and in particular relates to a Halomonas fannerii strain JPT10-1 for improving the salt-tolerant ability of corn, as well as its bacterial agent and application. Background technique [0002] Soil salinization is an important factor affecting agricultural production and ecological environment, and seriously affects crop planting and production. According to the incomplete statistics of the United Nations Educational, Scientific and Cultural Organization (UNESCO) and the United Nations Food and Agriculture Organization (FAO), the area of ​​saline soil in the world is 9.548×10 8 hm 2 , except for Antarctica which has not been surveyed, the rest of the continents have saline soil distribution, and the annual rate is 1.0×10 6 -1.5×10 6 hm 2 speed is growing. In my country, the saline-alkali soil has a wide distribution range, a large area, and many types, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 高峥王永东杨兴洪王令帅李祥
Owner SHANDONG AGRICULTURAL UNIVERSITY
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