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Bacillus subtilis strain and application thereof

A technology of Bacillus subtilis and strains, which is applied to Bacillus subtilis strains and their application fields, and achieves the effects of broad application prospects, bright green color and prolonged storage period

Active Publication Date: 2019-03-19
BEIJING FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For this reason, the embodiment of the present invention provides a kind of Bacillus subtilis bacterial strain and its application, to solve the rare problem in the prior art that the bacterial strain that obtains better effect in inhibiting Aspergillus carbon niger, Aspergillus niger, Penicillium chrysogenum is found

Method used

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  • Bacillus subtilis strain and application thereof
  • Bacillus subtilis strain and application thereof

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Embodiment 1

[0026] The present embodiment provides a kind of preparation method of Bacillus subtilis high-density fermented liquid comprising the steps:

[0027] 1) Preparation of strain seed solution: activate Bacillus subtilis on NA plate medium, take a single colony and streak it on NA medium, seal the glue and place it upside down in a 33°C incubator for 24 hours to obtain activated strains, transfer bacteria with Transfer the activated strains to LB medium, culture at 41.5°C and 240r / min for 37 hours, and store the prepared strain seed liquid at 1.5°C for later use;

[0028] 2) Preparation of high-density fermented liquid: Inoculate the strain seed liquid prepared in step 1) in an Erlenmeyer flask of high-density medium with an inoculum size of 4.5% (V / V). Under the condition of 36 hours of cultivation, the number of live bacteria of Bacillus subtilis CGMCC NO.16569 reached 3.27×10 11 CFU / mL. Obtain the bacterial suspension, centrifuge the bacterial suspension at 10,000 r / min, take...

Embodiment 2

[0030] The present embodiment provides a kind of preparation method of Bacillus subtilis high-density fermented liquid comprising the steps:

[0031] 1) Preparation of strain seed solution: activate Bacillus subtilis on NA plate medium, take a single colony and streak it on NA medium, seal the glue and place it upside down in a 33°C incubator for 24 hours to obtain activated strains, transfer bacteria with Transfer the activated strains to LB medium, cultivate them at 33°C and 180r / min for 24 hours, and store the prepared strains at -1°C for later use;

[0032] 2) Preparation of high-density fermented liquid: the strain seed liquid prepared in step 1) was inoculated in a conical flask of high-density medium with an inoculum size of 3% (V / V), and the temperature was 33°C and 180°C. Under the condition of 1 / min, after 24 hours of cultivation, the number of live bacteria of Bacillus subtilis CGMCC NO.16569 reached 3.41×10 11 CFU / mL. Obtain the bacterial suspension, centrifuge t...

Embodiment 3

[0034] The present embodiment provides a kind of preparation method of Bacillus subtilis high-density fermented liquid comprising the steps:

[0035] 1) Preparation of strain seed solution: activate Bacillus subtilis on NA plate medium, take a single colony and streak it on NA medium, seal the glue and place it upside down in a 33°C incubator for 24 hours to obtain activated strains, transfer bacteria with Transfer the activated strains to LB medium, culture at 50°C and 300r / min for 50h, and store the obtained strains at 4°C for later use;

[0036] 2) Preparation of high-density fermented liquid: the strain seed liquid prepared in step 1) is inoculated in a conical flask of high-density medium with an inoculum size of 6% (V / V), and the temperature is 50°C, 300r Under the condition of 48 hours of cultivation, the number of live bacteria of Bacillus subtilis CGMCC NO.16569 reached 3.60×10 11 CFU / mL. Obtain the bacterial suspension, centrifuge the bacterial suspension at 10,000...

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Abstract

The embodiment of the invention discloses a bacillus subtilis strain and application thereof. The bacillus subtilis strain is collected in China General Microbiological Culture Collection Center, hasa collection number of CGMCC NO. 16569, and has a collection date of October 10, 2018. The embodiment of the invention further provides high-density bacillus subtilis fermentation liquor containing bacillus subtilis strain metabolite. The bacillus subtilis strain provided by the embodiment of the invention is capable of effectively inhibiting aspergillus carbonarius, aspergillus niger and penicillium chrysogenum, while the aspergillus carbonarius, aspergillus niger and penicillium chrysogenum are mold that easily grow on grapes. Therefore, the high-density bacillus subtilis fermentation liquorcontaining the bacillus subtilis strain metabolite is capable of effectively prolonging the grape storage period, has the characteristics of being high-efficiency, safe, environment-friendly and thelike, and has wide application prospects.

Description

technical field [0001] The embodiments of the present invention relate to the technical field of microbial application, and in particular to a strain of Bacillus subtilis and its application. Background technique [0002] Bacillus subtilis is a kind of Bacillus, which has the functions of preventing biological oxidation, antagonizing pathogenic microorganisms, improving internal and external ecological environment, enhancing animal immune function, and producing various digestive enzymes and nutrients. During the growth of Bacillus subtilis, active substances such as subtilisin, polymyxin, nystatin, and gramicidin can be produced that have obvious inhibitory effects on pathogenic bacteria or opportunistic pathogenic bacteria of endogenous infection. Bacillus subtilis in the intestinal tract can quickly consume free oxygen, thereby reducing intestinal oxygen, allowing beneficial anaerobic bacteria to survive better and inhibiting some pathogenic bacteria. Studies have shown ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23B7/155C12R1/125
CPCA23B7/155A23V2002/00C12N1/20C12N1/205C12R2001/125A23V2200/10
Inventor 范俊峰张波陈敏宋佩泽周发牛琳茹
Owner BEIJING FORESTRY UNIVERSITY
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