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Bacillus methylotrophicus BMF 04, fermentation method for same and application of bacillus methylotrophicus BMF 04

A methylotrophic and Bacillus technology, applied in the field of marine bacteria, can solve problems such as the lack of types of biological pesticides that cannot meet production needs, pesticide residues, and resistance of harmful organisms

Active Publication Date: 2019-03-19
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is aimed at problems such as pesticide residues, environmental pollution, and pest resistance caused by the current application of chemical pesticides to prevent and control plant diseases. Biological pesticides can make up for the shortcomings of chemical pesticides, but there are few types of biological pesticides on the market. To meet the deficiencies of existing technologies such as production needs, provide a new methylotrophic Bacillus (Bacillus methylotrophicus) BMF 04 with strong antibacterial effect and prospects for the development and application of biological pesticides and fertilizers
[0004] Another technical problem to be solved by the present invention is to provide a low-cost, high-efficiency fermentation method for the aforementioned methylotrophic bacillus (Bacillus methylotrophicus) BMF 04 for the current situation that needs to save costs and improve fermentation efficiency in the production process

Method used

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  • Bacillus methylotrophicus BMF 04, fermentation method for same and application of bacillus methylotrophicus BMF 04
  • Bacillus methylotrophicus BMF 04, fermentation method for same and application of bacillus methylotrophicus BMF 04
  • Bacillus methylotrophicus BMF 04, fermentation method for same and application of bacillus methylotrophicus BMF 04

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1, Bacteriostatic experiment of Bacillus methylotrophicus BMF 04 strain: 1 Materials and methods

[0083] 1.1 Test material

[0084] 1.1.1 Test strains and pathogens

[0085] Bacillus methylotrophic strain BMF 04 was isolated from the sea area of ​​Lianyungang. The tested plant pathogens (see Table 8) are preserved in this laboratory.

[0086] 1.1.2 Culture medium

[0087] PDA medium: potato 200g, glucose 20g, agar 20g, water 1000mL;

[0088] PD medium: potato 200g, glucose 20g, water 1000mL;

[0089] BMF 04 strain fermentation medium: bran 1.0%, peanut flour 1.0%, CaCl 2 1.0%.

[0090] 1.1.3 Activation of BMF04 strain and pathogenic fungi

[0091] The BMF04 strain stored at 4°C was transferred to the PDA slant under sterile conditions, and cultured at 28°C for 1 d for activation; the phytopathogenic fungi stored at 4°C were transferred to the PDA slant under sterile conditions. above, incubate at 28 °C for 3 d for activation and set aside.

[0092] 1....

Embodiment 2

[0110] Embodiment 2, Bacillus methylotrophicus (Bacillus methylotrophicus) BMF 04 production of antibacterial substances fermentation medium and shake flask fermentation conditions optimization experiment:

[0111] 1. Experimental purpose: Optimization of fermentation medium and shake flask fermentation conditions for BMF 04 production of antibacterial substances

[0112] 2 Materials and methods

[0113] 2.1 Materials

[0114] 2.1.1 Test strains

[0115] Bacillus methylotrophicus BMF 04 strain, Fusarium gramminearum, was isolated, preserved and provided by our laboratory.

[0116] 2.1.2 Culture medium

[0117] (1) PDA medium: 200 g of fresh potatoes, 20 g of glucose, 20 g of agar, and 1000 mL of water.

[0118] (2) PD medium: 200 g of fresh potatoes, 20 g of glucose, and 1000 mL of water.

[0119] 2.2 Methods

[0120] 2.2.1 Strain activation

[0121] The strain of Bacillus methylotroph BMF 04 stored in a refrigerator at 4°C was transferred to a PDA slant medium, and cul...

Embodiment 3

[0206] Embodiment 3, Bacillus methylotroph BMF 04 solid-state fermentation medium formula and fermentation condition optimization experiment:

[0207] 1 The purpose of the experiment

[0208] In this experiment, the total number of bacteria and the spore rate in the fermented product were used as the detection indicators, and the effects of different carbon sources, nitrogen sources, inorganic salts and their concentrations on the solid-state fermentation of BMF 04 strain were studied through response surface methodology and single-factor experimental design, and the solid-state fermentation was optimized. Medium formula; based on the optimized solid-state fermentation medium, the solid-state fermentation conditions are optimized.

[0209] 2 Materials and methods

[0210] 2.1 Test material

[0211] 2.1.1 Test strains

[0212] Bacillus methylotroph BMF 04 was isolated, purified and preserved by our laboratory in the sea area of ​​Lianyungang.

[0213] 2.1.2 Culture medium ...

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Abstract

The invention discloses bacillus methylotrophicus BMF 04, further discloses application of the bacillus methylotrophicus BMF 04 to inhibiting plant pathogenic fungi, application of sterile fermentation liquor of strains to inhibiting the plant pathogenic fungi, application of the sterile fermentation liquor of the strains to preparing bacterium inhibition medicines and application of the sterile fermentation liquor to promoting growth of cucumber seedlings, discloses a preparation method for sterile fermentation liquor of the bacillus methylotrophicus BMF 04 and a fermentation culture method for producing bacterium inhibition substances and further discloses a solid-state fermentation culture method for producing spores from the bacillus methylotrophicus BMF 04. A preservation number of the bacillus methylotrophicus BMF 04 is CCTCC NO:2017375. The sterile fermentation liquor of the strains is used as an effective component for the bacterium inhibition medicines. The bacillus methylotrophicus BMF 04, the application, the preparation method, the fermentation culture method and the solid-state fermentation culture method have the advantages that the novel marine bacterium strains withbroad-spectrum resistance are screened pertinently for common pathogenic bacteria in crops, and species relations of the excellent resistant strains are determined by means of strain morphological observation, physiological and biochemical tests and 16S rDNA (ribosomal deoxyribonucleic acid) sequence analysis; research is further carried out on antibacterial application of the bacillus methylotrophicus BMF 04, and directions can be provided to application of the strains.

Description

technical field [0001] The present invention relates to a kind of marine bacteria, especially a kind of methylotrophic bacillus BMF 04, the present invention also relates to its use and fermentation method. Background technique [0002] Biological control has the advantages of not polluting the environment, being safe for humans and other organisms, having a lasting control effect, and having no residues in products. important. The existing microbial strains used for the development of microbial pesticides are mainly derived from the terrestrial environment. The development and research of microbial strains derived from the ocean started relatively late. The marine bacteria grow and multiply rapidly and have abundant metabolites, which are important germplasm resources for screening resistant strains. Different scholars have isolated several excellent strains of marine bacteria with strong antibacterial effects from the ocean. Zhu Xiaofei et al. screened strain YB-3, which...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/04A01P3/00A01P21/00C12R1/07
CPCA01N63/00A01N63/30C12N1/205C12R2001/07
Inventor 马桂珍陈新元吴海霞万丹丹李欢曹雪梅暴增海
Owner HUAIHAI INST OF TECH
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