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Immunoassay method and system and kit for identifying immunoassay

An immunoassay and immunoreaction technology, applied in the fields of immunoassay, identification immunoassay system, and kits, which can solve the problems of limiting the detection range and the inability of the signal to increase continuously.

Active Publication Date: 2019-03-15
BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] On the other hand, because of the high dose-hook effect, when the sample concentration increases to a certain value, the signal cannot continue to increase, which limits the detection range

Method used

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  • Immunoassay method and system and kit for identifying immunoassay
  • Immunoassay method and system and kit for identifying immunoassay
  • Immunoassay method and system and kit for identifying immunoassay

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0144] Example 1: Detection of human chorionic gonadotropin and β subunit (HCG+β) in human serum samples to verify the effectiveness of the method of the present invention

[0145] Human chorionic gonadotropin and β subunit (HCG+β) detection kit (photo-induced chemiluminescence method) produced by Boyang Biotechnology (Shanghai) Co., Ltd. was used to detect human chorionic gonadotropin and β in serum samples Subunit (HCG+β) content. The kit includes calibrator 1-calibrator 6, reagent 1 (luminescent antibody, that is, antibody-coated luminescent particles), and reagent 2 (biotin-labeled antibody, that is, biotin-labeled antibody).

[0146] Get 18 routine HCG+β concentration patient serum samples by Roche detection (exceed detection limit sample and carry out dilution detection), carry out conventional method and the method detection of the present invention respectively,

[0147] Routine detection method: the sample to be tested, calibrator, reagent 1 (luminescent antibody, th...

Embodiment 2

[0155] Embodiment 2: detection of ferritin (Ferr) in the sample verifies the validity of the method of the present invention

[0156] The ferritin (Ferr) detection kit (chemiluminescence method) produced by Boyang Biotechnology (Shanghai) Co., Ltd. was used to detect the content of ferritin (purchased from Fitzgerald, Catalog No: 30-AF10) in the sample. The kit includes calibrator 1-calibrator 6, reagent 1 (luminescent antibody, that is, antibody-coated luminescent particles), and reagent 2 (biotin-labeled antibody, that is, biotin-labeled antibody).

[0157] Calibrator 1-Calibrator 6: Samples with known concentrations in conventional kits, the concentration is much lower than that of HOOK samples, and a standard curve is used to calculate the concentration of the analyte.

[0158] Other components to be used: LiCA universal solution (streptavidin-labeled photosensitive particles), which is an auxiliary reagent for the production of light-induced chemiluminescence analysis sys...

Embodiment 3

[0168] Example 3: C-peptide (CP) in the detection sample verifies the effectiveness of the method of the present invention

[0169] The C-peptide (CP) detection kit (chemiluminescence method) produced by Boyang Biotechnology (Shanghai) Co., Ltd. was used to detect the content of C-peptide (purchased from Fitzgerald, Catalog No: 30-AC96) in the sample. The kit includes calibrator 1-calibrator 6, reagent 1 (luminescent antibody, that is, antibody-coated luminescent particles), and reagent 2 (biotin-labeled antibody, that is, biotin-labeled antibody).

[0170] Calibrator 1-Calibrator 6: Samples with known concentrations in conventional kits, the concentration is much lower than that of HOOK samples, and a standard curve is used to calculate the concentration of the analyte.

[0171] The high-concentration C-peptide antigen is serially diluted, and the concentration values ​​of samples containing different concentrations of C-peptide are determined by conventional detection method...

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Abstract

The invention relates to a system for identifying immunoassay in the technical field of light initiated chemiluminescence. The system comprises an immunoreaction device, a chemiluminescence immunoreaction stimulating and counting device and a processor, wherein the immunoreaction device is used for implementing chemiluminescence immunoreaction; the chemiluminescence immunoreaction stimulating andcounting device is used for stimulating and recording first-time reading and second-time reading of chemiluminescence, and recording the amplification of the difference between the second-time readingand the first-time reading as A; and the processor is used for comparing the amplification A of the two-time readings of a to-be-measured sample which contains a to-be-measured target antigen (or antibody) with a critical value, if the amplification A of the two-time readings of the to-be-measured sample which contains the to-be-measured target antigen (or antibody) is greater than the critical value, then the concentration of the to-be-measured sample is higher than the concentration of a known standard substance; and meanwhile, a signal value obtained by the first-time reading of the to-be-measured sample is lower than the known standard substance, and the sample is diluted and then measured.

Description

[0001] This application is a divisional application of a Chinese patent application with an application date of November 22, 2016, an application number of 201611026623.1, and an invention title of "immunoassay method, system and kit for identifying immunoassay". technical field [0002] The present invention relates to the technical field of light-activated chemiluminescence, in particular to an immunoassay method, a system for identifying an immunoassay, and a kit; another immunoassay method, another system for identifying an immunoassay system and another kit. Background technique [0003] Immunological detection is based on the principle of antigen-antibody specific reaction. Because it can use isotopes, enzymes, chemiluminescent substances, etc. to display or amplify the signal of the measured substance, it is often used to detect trace organisms such as proteins and hormones. active substance. [0004] Chemiluminescent immunoassay is a non-radioactive immunoassay tech...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N21/76
CPCG01N21/76G01N33/5761G01N33/5764G01N33/5767G01N33/58G01N33/6854G01N33/6893G01N33/76
Inventor 杨阳赵卫国张向辉
Owner BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
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