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Immunoassay method and system and kit for identifying immunoassay

An immunoassay and kit technology, applied in the field of photoexcited chemiluminescence, can solve the problems of limiting the detection range and the signal cannot be continuously increased.

Active Publication Date: 2019-03-15
BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] On the other hand, because of the high dose-hook effect, when the sample concentration increases to a certain value, the signal cannot continue to increase, which limits the detection range

Method used

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  • Immunoassay method and system and kit for identifying immunoassay
  • Immunoassay method and system and kit for identifying immunoassay
  • Immunoassay method and system and kit for identifying immunoassay

Examples

Experimental program
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Effect test

Embodiment 1

[0138] Embodiment 1: Hepatitis B virus surface antibody (HBsAb) verifies the validity of the method of the present invention in the detection sample

[0139] The hepatitis B virus surface antibody detection kit HBsAbHBsAb (photo-induced chemiluminescence method) produced by Boyang Biotechnology (Shanghai) Co., Ltd. was used to detect the hepatitis B virus surface antibody in the sample (purchased from Beijing Zhongke Jingda Biotechnology Co., Ltd. company, Clone No: M2201). The kit includes calibrator 1 - calibrator 6, reagent 1 (luminescent particles coated with HBsAg) and reagent 2 (biotin-labeled HBsAg).

[0140] Calibrator 1-Calibrator 6: Samples with known concentrations in conventional kits, the concentration is much lower than that of HOOK samples, and a standard curve is used to calculate the concentration of the analyte.

[0141] The high-concentration HBsAb is serially diluted, and the concentration values ​​of samples containing different concentrations of HBsAb ar...

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Abstract

The invention relates to an immunoassay method for a hepatitis B virus surface antibody, a system for identifying immunoassay of the hepatitis B virus surface antibody and a kit for detecting the hepatitis B virus surface antibody in the technical field of light initiated chemiluminescence, and another immunoassay method for the hepatitis B virus surface antibody, a system for identifying immunoassay of the hepatitis B virus surface antibody and another kit for detecting the hepatitis B virus surface antibody.

Description

[0001] This application is a divisional application of a Chinese patent application with an application date of November 22, 2016, an application number of 201611026623.1, and an invention title of "immunoassay method, system and kit for identifying immunoassay". technical field [0002] The invention relates to the technical field of light-induced chemiluminescence, in particular to an immunoassay method for hepatitis B virus surface antibody, an immunoassay system for identifying hepatitis B virus surface antibody, and a method for detecting hepatitis B virus surface antibody and another immunoassay method for hepatitis B virus surface antibody, another immunoassay system for identifying hepatitis B virus surface antibody, and another kit for detecting hepatitis B virus surface antibody. Background technique [0003] Immunological detection is based on the principle of antigen-antibody specific reaction. Because it can use isotopes, enzymes, chemiluminescent substances, etc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/576G01N21/76
CPCG01N21/76G01N33/5761G01N33/5764G01N33/5767G01N33/58G01N33/6854G01N33/6893G01N33/76
Inventor 杨阳赵卫国张向辉
Owner BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
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