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Immunoassay method, system for identifying immunoassay and kit

A kit and antibody technology, applied in the field of photoexcited chemiluminescence, can solve the problems of limiting the detection range and the signal cannot be continuously increased.

Inactive Publication Date: 2019-03-15
BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] On the other hand, because of the high dose-hook effect, when the sample concentration increases to a certain value, the signal cannot continue to increase, which limits the detection range

Method used

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  • Immunoassay method, system for identifying immunoassay and kit
  • Immunoassay method, system for identifying immunoassay and kit

Examples

Experimental program
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Effect test

Embodiment 1

[0144] Example 1: Detection of human chorionic gonadotropin and β subunit (HCG+β) in human serum samples to verify the effectiveness of the method of the present invention

[0145] Human chorionic gonadotropin and β subunit (HCG+β) detection kit (photo-induced chemiluminescence method) produced by Boyang Biotechnology (Shanghai) Co., Ltd. was used to detect human chorionic gonadotropin and β in serum samples Subunit (HCG+β) content. The kit includes calibrator 1-calibrator 6, reagent 1 (luminescent antibody, that is, antibody-coated luminescent particles), and reagent 2 (biotin-labeled antibody, that is, biotin-labeled antibody).

[0146] Get 18 routine HCG+β concentration patient serum samples by Roche detection (exceed detection limit sample and carry out dilution detection), carry out conventional method and the method detection of the present invention respectively,

[0147] Routine detection method: the sample to be tested, calibrator, reagent 1 (luminescent antibody, th...

Embodiment 2

[0155] Embodiment 2: detection of ferritin (Ferr) in the sample verifies the validity of the method of the present invention

[0156] The ferritin (Ferr) detection kit (chemiluminescence method) produced by Boyang Biotechnology (Shanghai) Co., Ltd. was used to detect the content of ferritin (purchased from Fitzgerald, Catalog No: 30-AF10) in the sample. The kit includes calibrator 1-calibrator 6, reagent 1 (luminescent antibody, that is, antibody-coated luminescent particles), and reagent 2 (biotin-labeled antibody, that is, biotin-labeled antibody).

[0157] Calibrator 1-Calibrator 6: Samples with known concentrations in conventional kits, the concentration is much lower than that of HOOK samples, and a standard curve is used to calculate the concentration of the analyte.

[0158] Other components to be used: LiCA universal solution (streptavidin-labeled photosensitive particles), which is an auxiliary reagent for the production of light-induced chemiluminescence analysis sys...

Embodiment 3

[0168] Example 3: C-peptide (CP) in the detection sample verifies the effectiveness of the method of the present invention

[0169] The C-peptide (CP) detection kit (chemiluminescence method) produced by Boyang Biotechnology (Shanghai) Co., Ltd. was used to detect the content of C-peptide (purchased from Fitzgerald, Catalog No: 30-AC96) in the sample. The kit includes calibrator 1-calibrator 6, reagent 1 (luminescent antibody, that is, antibody-coated luminescent particles), and reagent 2 (biotin-labeled antibody, that is, biotin-labeled antibody).

[0170] Calibrator 1-Calibrator 6: Samples with known concentrations in conventional kits, the concentration is much lower than that of HOOK samples, and a standard curve is used to calculate the concentration of the analyte.

[0171] The high-concentration C-peptide antigen is serially diluted, and the concentration values ​​of samples containing different concentrations of C-peptide are determined by conventional detection method...

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Abstract

The invention relates to a kit in the technical field of light activated chemiluminiscence. The kit comprises luminous particles coated with a first antibody (or antigen), a second antibody (or antigen) marked by a marker and light-sensitive particles marked by a marker specific conjugate. A use method of the kit comprises the steps of (1) carrying out chemiluminiscence immunoreaction on a sampleto be detected, which contains a target antigen (or antibody) to be detected; activating and recording a first reading and a second reading of chemiluminiscence; and recording a difference increasingrate between the second reading and the first reading as A; (2) using increasing rate A' of the two readings of one known standard substance containing the target antigen (or antibody) to be detectedas a critical value; and (3) comparing the increasing rate A of the two readings of the sample to be detected, which contains the target antigen (or antibody) to be detected, with the critical value,wherein if the increasing rate A of the two readings of the sample to be detected, which contains the target antigen (or antibody) to be detected, is larger than the critical value, the concentrationof the sample to be detected is higher than the concentration of the known standard substance.

Description

[0001] This application is a divisional application of a Chinese patent application with an application date of November 22, 2016, an application number of 201611026623.1, and an invention title of "immunoassay method, system and kit for identifying immunoassay". technical field [0002] The present invention relates to the technical field of light-activated chemiluminescence, in particular to an immunoassay method, a system for identifying an immunoassay, and a kit; another immunoassay method, another system for identifying an immunoassay system and another kit. Background technique [0003] Immunological detection is based on the principle of antigen-antibody specific reaction. Because it can use isotopes, enzymes, chemiluminescent substances, etc. to display or amplify the signal of the measured substance, it is often used to detect trace organisms such as proteins and hormones. active substance. [0004] Chemiluminescent immunoassay is a non-radioactive immunoassay tech...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N21/76
CPCG01N21/76G01N33/5761G01N33/5764G01N33/5767G01N33/58G01N33/6854G01N33/6893G01N33/76
Inventor 杨阳赵卫国张向辉
Owner BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
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