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Immunoassay method and system and kit for identifying immunoassay

A technique of immunoassay and chemiluminescence immunity, applied in the field of photoexcited chemiluminescence, which can solve the problems of limiting the detection range and the inability to continuously increase the signal.

Active Publication Date: 2019-03-15
BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0009] On the other hand, because of the high dose-hook effect, when the sample concentration increases to a certain value, the signal cannot continue to increase, which limits the detection range

Method used

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  • Immunoassay method and system and kit for identifying immunoassay
  • Immunoassay method and system and kit for identifying immunoassay
  • Immunoassay method and system and kit for identifying immunoassay

Examples

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Effect test

Embodiment 1

[0138] Embodiment 1: detection of ferritin (Ferr) in the sample verifies the validity of the method of the present invention

[0139] The ferritin (Ferr) detection kit (chemiluminescence method) produced by Boyang Biotechnology (Shanghai) Co., Ltd. was used to detect the content of ferritin (purchased from Fitzgerald, Catalog No: 30-AF10) in the sample. The kit includes calibrator 1-calibrator 6, reagent 1 (luminescent antibody, that is, antibody-coated luminescent particles), and reagent 2 (biotin-labeled antibody, that is, biotin-labeled antibody).

[0140] Calibrator 1-Calibrator 6: Samples with known concentrations in conventional kits, the concentration is much lower than that of HOOK samples, and a standard curve is used to calculate the concentration of the analyte.

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Abstract

The invention relates to an immunoassay method for ferritin, a system for identifying ferritin immunoassay and a kit for detecting ferritin in the technical field of light initiated chemiluminescence,and another immunoassay method for ferritin, another system for identifying ferritin immunoassay and another kit for detecting ferritin.

Description

[0001] This application is a divisional application of a Chinese patent application with an application date of November 22, 2016, an application number of 201611026623.1, and an invention title of "immunoassay method, system and kit for identifying immunoassay". technical field [0002] The present invention relates to the technical field of light-induced chemiluminescence, in particular to an immunoassay method for ferritin, a system for identifying ferritin immunoassay and a kit for detecting ferritin; and another immunoassay for ferritin Assay methods, another system for the identification of ferritin immunoassays and another kit for the detection of ferritin. Background technique [0003] Immunological detection is based on the principle of antigen-antibody specific reaction. Because it can use isotopes, enzymes, chemiluminescent substances, etc. to display or amplify the signal of the measured substance, it is often used to detect trace organisms such as proteins and ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N21/76
CPCG01N21/76G01N33/5761G01N33/5764G01N33/5767G01N33/58G01N33/6854G01N33/6893G01N33/76
Inventor 杨阳赵卫国张向辉
Owner BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
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