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Chemiluminescence analysis and determination method, system using same and kit

A technology of chemiluminescence and determination method, applied in the field of chemiluminescence, which can solve problems such as misdiagnosis and false negative rate increase

Active Publication Date: 2019-11-29
BEYOND DIAGNOSTICS (SHANGHAI) CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the existence of the HD-HOOK effect, the detected sample cannot be correctly distinguished whether its concentration exceeds the linear range of the detection kit or the concentration itself is the value, so that the experiment is misdiagnosed, especially resulting in an increase in the false negative rate

Method used

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  • Chemiluminescence analysis and determination method, system using same and kit
  • Chemiluminescence analysis and determination method, system using same and kit
  • Chemiluminescence analysis and determination method, system using same and kit

Examples

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Embodiment 1

[0259] Embodiment 1: Conventional method and method of the present invention detect alpha-fetoprotein (AFP) sample respectively

[0260] In this example, the alpha-fetoprotein (AFP) detection kit (chemiluminescent method) produced by Boyang Biotechnology (Shanghai) Co., Ltd. was used to detect the content of alpha-fetoprotein in the sample.

[0261] The high-concentration alpha-fetoprotein antigen is serially diluted, and the signal values ​​of samples containing different concentrations of alpha-fetoprotein are measured by conventional detection methods and the detection method of the present invention.

[0262] Conventional detection methods include the following steps:

[0263] A. The analyte sample (known standard substance) of known concentration is mixed with reagent 1 (receptor solution combined with mouse monoclonal antibody) and reagent 2 (mouse monoclonal antibody solution combined with biotin), Incubate at 37°C for 10 minutes; then add reagent 3 (donor solution com...

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Abstract

The invention relates to a chemiluminiscence analysis and determination method, a system using the same and a kit and belongs to the technical field of chemiluminiscence. According to the method disclosed by the invention, a detection range is widen through a plurality of times of reading; and the concentration of a to-be-detected substance is simply, conveniently and quickly calculated.

Description

technical field [0001] The invention relates to the technical field of chemiluminescence, in particular to a chemiluminescence analysis and determination method, a system using the chemiluminescence analysis and determination method and a kit. Background technique [0002] Chemiluminescent analysis is a non-radioactive immunoassay technology that has developed rapidly in recent years. Its principle is to use chemiluminescent substances to amplify the signal, and use its luminous intensity to directly measure the process of immune binding. This method has become an immunological detection method. one of the important directions. Light-induced chemiluminescence is one of the commonly used methods of chemiluminescence analysis technology, which can be used to study the interaction between biomolecules, and is mainly used in the detection of diseases clinically. This technology integrates the research of related fields such as polymer particle technology, organic synthesis, pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N33/543
CPCG01N21/76G01N33/543
Inventor 杨阳赵卫国刘宇卉李临
Owner BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
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