Filling material for bone defect repairing and preparation method

A technology for filling materials and bone defects, applied in pharmaceutical formulations, pharmaceutical science, capsule delivery, etc., can solve the problems of poor treatment effect and high price, and achieve the effect of reducing treatment time and pain, low price and strong plasticity

Inactive Publication Date: 2019-03-15
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few types of bone graft materials in the pet medi

Method used

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  • Filling material for bone defect repairing and preparation method
  • Filling material for bone defect repairing and preparation method
  • Filling material for bone defect repairing and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Preparation of Rabbit Demineralized Bone Matrix

[0038] Add 30 mL / g of rabbit bone to a chloroform / methanol (1:1) solution to degrease for 8 hours, pour off the organic solvent, and rinse with double distilled water for 3 times. Dehydrate with ethanol 5 times the volume of the bone block for 2 h. Put the bone into a deep-low temperature refrigerator to freeze for 4 hours, take it out and grind it in a grinder with liquid nitrogen at low temperature, and use a sample sieve to sieve bone particles of 200-800 μm in size. Put bone particles into 0.6M hydrochloric acid solution for decalcification according to the ratio of dilute hydrochloric acid to bone weight of 20:1, stir every half hour, and replace with new decalcification solution every 8 hours, and decalcification is completed in about 24 hours. Rinse with double distilled water until the pH is around 7.0. Degrease again with chloroform / methanol (1:1) for 4 hours, wash with double distilled water 3 time...

Embodiment 2

[0039] Embodiment 2: flavone sustained-release microcapsules

[0040] Use a pipette to accurately draw 0.4 mL of flavone solution as the inner aqueous phase. Under vortex stirring, the flavonoid solution was rapidly added dropwise to a 140 mg / mL PLGA solution in 4 mL of dichloromethane, and quickly transferred to a homogenizer at a speed of 1500 rpm, stirred and emulsified for 5 min to form colostrum. Then add the colostrum into 10mL of 1% polyvinyl alcohol solution, stir and emulsify at high speed in a vortex for several minutes to form double emulsion. Move it to 20mL of 1% polyvinyl alcohol solution in a fume hood and magnetically stir for 4h to remove methylene chloride, centrifuge at 5000rpm in a high-speed centrifuge for 10min, collect the supernatant, take the microcapsules at the bottom of the test tube, wash with double distilled water, dry and Freeze-drying yields a light yellow powder.

Embodiment 3

[0041] Example 3: Preparation of Plastic Bone Clay for Grafting

[0042] 1) Under room temperature, in a clean operating table, accurately weigh carboxymethylcellulose (CMC) and demineralized bone matrix (DBM) and mix them evenly, and grind them into powder;

[0043] 2) adding flavonoid slow-release microcapsules to the mixture in step 2) to make a finished package;

[0044] 3) The mixture was sterilized under 25kGy gamma rays and stored at -20°C for future use.

[0045]Accurately weigh and mix according to the ratio of CMC:DBM=130mg:870mg, divide them into 4 groups at random, each group has the same bone cement weight. Add high (2.4mg), medium (1.2mg) and low (0.6mg) doses of flavonoid PLGA microcapsules to three groups, make finished products in a clean bench, and store them at -20°C for later use.

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Abstract

The invention relates to a filling material for bone defect repairing and a preparation method. The filling material comprises carboxymethyl cellulose, a decalcified bone matrix and a slow-release flavone microcapsule, wherein the weight ratio of the carboxymethyl cellulose to the decalcified bone matrix is 13:87; in addition, counted by the total weight of the carboxymethyl cellulose and the decalcified bone matrix, 0.03-0.12% of the slow-release flavone microcapsule is added; during preparation, the preparation method comprises the following steps: accurately weighing the prepared decalcified bone matrix and the prepared carboxymethylcellulose, uniformly mixing, adding the slow-release flavone microcapsule therein, preparing a finished product, packaging, performing irradiation sterilization, and preserving at -20 DEG C to obtain plastic transplanted bone mud. The filling material is stable in physical properties, strong in plasticity and convenient to use; the filling material promotes growth of new bone and disappearance of a fracture line and is more closely connected with a host bone; the filling material is a bone repairing material which is cheap, safe, effective, convenient to use and can be preserved for a long time, and can shorten the treatment time and reduce the pain of a sick pet.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a filling material for bone defect repair and a preparation method. Background technique [0002] Demineralized bone matrix (DBM) is a bone matrix product processed by allogeneic or heterogeneous bone decalcification and degreasing. DBM has a good three-dimensional space structure and satisfactory biocompatibility, and also has the ability of osteoinduction, osteoconduction and osteogenesis, and is widely used in clinical bone grafting operations. American scientist Urist proposed the landmark osteoinductive theory in 1965, developed decalcified bone matrix after decalcification and de-antigen treatment, and first established a complete and reliable animal test model, implanting DBM into muscle or subcutaneous site, and successfully induced osteogenesis. Finally, in 1982, bovine bone morphogenetic protein (BMP) was successfully extracted, which also proved that B...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/20A61L27/36A61L27/54
CPCA61L27/18A61L27/20A61L27/36A61L27/3608A61L27/365A61L27/54A61L2300/30A61L2300/412A61L2300/602A61L2300/622A61L2430/02C08L67/04C08L1/286
Inventor 丁明星丁一朱红梅田杭宇张秋林明洁孙晋睿
Owner HUAZHONG AGRI UNIV
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