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Gardenia extract for treating hyperbileacidemia and preparation and application thereof

A technology of gardenia extract and bile acid, applied in the field of gardenia extract and its preparation and application, can solve the problems such as no relevant reports, and achieve the effects of improving the scope of application, reducing costs, and improving drug safety.

Active Publication Date: 2019-03-15
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the pathogenesis of cholestasis and hyperbiliary acidemia are not the same, and there is no relevant report on the effect and mechanism of gardenia extract in the treatment of hyperbiliary acidemia

Method used

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  • Gardenia extract for treating hyperbileacidemia and preparation and application thereof
  • Gardenia extract for treating hyperbileacidemia and preparation and application thereof
  • Gardenia extract for treating hyperbileacidemia and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 gardenia extract and its preparation

[0030] Preparation:

[0031](1) Add 2 times the volume of 80% ethanol to soak the gardenia for 0.5 hours, reflux at 60°C for 3 hours, filter, and collect the filtrate; after filtering, add 2 times the volume of 80% ethanol to extract for 3 hours at 60°C. Filter, combine the filtrates, concentrate until there is no alcohol smell, and obtain Gardenia extract;

[0032] (2) Gardenia extract was added to AB-8 macroporous resin column, 2 times column volume was eluted with water, and the eluent was discarded; 3 times column volume was eluted with 8% ethanol, and the eluent was discarded; Elute with 40% ethanol by volume, collect the 40% ethanol eluate, concentrate under reduced pressure, and dry to obtain the product.

[0033] Content detection:

[0034] (1) Instruments and reagents

[0035] Instrument: HPLC-Shimadzu 20AT, including Shimadzu LC-20AT pump, SIL-20A constant temperature autosampler, CTO-20A column thermosta...

Embodiment 2

[0056] Embodiment 2 gardenia extract and its preparation

[0057] Preparation:

[0058] (1) Add 10 times the volume of gardenia 30% ethanol after crushing, soak for 2 hours, reflux at 50°C for 1 hour, filter, and collect the filtrate; after filtering, add 10 times the volume of gardenia 30% ethanol, extract at 50°C for 1 hour, filter , combined the filtrates, and concentrated until there was no alcohol smell to obtain Gardenia extract.

[0059] (2) Add gardenia extract into AB-8 macroporous resin column, elute with 10 times column volume of water, discard eluent; 9 times column volume of 3% ethanol elute, discard eluent; Elute with 25% ethanol by volume, collect the 25% ethanol eluate, concentrate under reduced pressure, and dry to obtain the product.

[0060] Content detection and qualitative detection are with embodiment 1.

[0061] The total amount of active ingredients in the gardenia extract was measured to be 69.3%, and the active ingredients included geniposide, geni...

Embodiment 3

[0062] Embodiment 3 gardenia extract and its preparation

[0063] (1) Add 8 times the volume of gardenia 60% ethanol after crushing, soak for 1 hour, reflux at 55°C for 2 hours, filter, and collect the filtrate; after filtering, add 5 times the volume of gardenia 60% ethanol, extract at 55°C for 2 hours, filter , combined the filtrates, and concentrated until there was no alcohol smell to obtain Gardenia extract.

[0064] (2) Add gardenia extract into AB-8 macroporous resin column, elute with water for 5 times of the column volume, discard the eluent; elute with 5% ethanol for 6 times of the column volume, discard the eluent; Elute with 30% ethanol by volume, collect the 30% ethanol eluate, concentrate under reduced pressure, and dry to obtain the product.

[0065] Content detection and qualitative detection are the same as in Example 1.

[0066] After testing, the total amount of active ingredients in the gardenia extract is 92.6%, including geniposide, genipin 1-β-D-long m...

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Abstract

The invention relates to a gardenia extract, in particular to a gardenia extract for treating hyperbileacidemia. The gardenia extract comprises geniposide, genipin 1-beta-D-gentiobioside, 6''-p-coumaroyl genipin gentiobioside, scandoside methyl ester, geniposidic acid, gardenoside and gardenoside, and is prepared through ethanol extraction and macroporous resin purification. The gardenia extract is capable of significantly reducing the bile acid level in the blood of rats, has a good effect on the treatment of hyperbileacidemia, and can be applied to the preparation of drugs for treating hyperbileacidemia.

Description

technical field [0001] The invention relates to a gardenia extract and its preparation and application, in particular to a gardenia extract for treating hyperbiliary acidemia and its preparation and application. Background technique [0002] Gardenia is the fruit of Rubiaceae gardenia, also known as yellow gardenia, mountain gardenia, and white toad. The fruit of gardenia is a traditional Chinese medicine, which belongs to the first batch of medicinal and edible resources promulgated by the Ministry of Health. [0003] The research on gardenia is very extensive. Previous pharmacodynamic studies have shown that gardenia has the following effects: (1) Effects on liver function: gardenia can reduce serum bilirubin content; Red pigment level can prevent fulminant hepatitis caused by galactosamine, and can reduce serum bilirubin, alanine aminotransferase and alkaline phosphatase in rats poisoned by a-naphthyl isothiocyanate. (2) Effect on bile secretion: Gardenia jasmine has ch...

Claims

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Application Information

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IPC IPC(8): A61K36/744A61P1/16
CPCA61K36/744A61K2236/333A61K2236/39A61K2236/55A61P1/16
Inventor 梁爱华赵雍李春英易艳王宏洁边宝林
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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