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Method for creating trichoderma mutant by using EMS mutagenesis and application

A mutant, Trichoderma technology, applied in the field of microorganisms, can solve the problems of low success rate, unsatisfactory Trichoderma mutagenesis effect, etc., and achieve a remarkable effect of mutagenesis.

Inactive Publication Date: 2019-03-08
HUNAN AGRI BIOTECH RES CENT
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, for the construction of mutants and mutation breeding of Trichoderma mainly through physical (ARTP technology and radiation) mutagenesis, the success rate is not high; the transposon method (indel) is not sensitive to antibiotics and other reasons, and many Trichoderma mutagenesis effects are not good. Ideal; and EMS mutagenesis technology to construct a library of Trichoderma mutants has not yet been reported

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  • Method for creating trichoderma mutant by using EMS mutagenesis and application
  • Method for creating trichoderma mutant by using EMS mutagenesis and application
  • Method for creating trichoderma mutant by using EMS mutagenesis and application

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Embodiment Construction

[0037] In order to enable those skilled in the art to better understand the present invention, the technical solution of the present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0038] refer to Figure 1-7 The embodiment of the present invention will be further described.

[0039] The implementation of the present invention will be illustrated by specific specific examples below, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0040] Adopt the method for EMS mutagenesis to create Trichoderma mutant, comprise the steps:

[0041] 1.1 Material selection

[0042] The tested strain Trichoderma T216 (China Center for Type Culture Collection, CCTCC№: M207141); Fusarium oxysporum Fon-1 (gifted by Researcher Xu Yong from Vegetable Research Center, Beijing Academy of Agriculture and Forestry); EMS mutagen was produc...

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Abstract

The invention discloses a method for creating a trichoderma mutant by using EMS mutagenesis and application. The method comprises the following steps: material selection, preparation of a spore suspension, preparation of an EMS phosphate buffer, selection and mutagenesis process for the optimal conditions of EMS mutagenesis, screening of phenotypic variation mutant strains, screening of antagonismcapacity variation mutant strains and storage of mutant strains. A trichoderma mutant library is constructed by using the technology of EMS mutagenesis, a quick and efficient mutagenesis technique isobtained, and a technical system for obtaining stable inheritance mutants is realized.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a method and an application of using EMS mutagenesis to create a Trichoderma mutant. Background technique [0002] Trichoderma is generally considered to be the most likely biocontrol factor to replace a variety of chemical fungicides in the world. In addition to preventing and controlling soil-borne diseases and some leaf diseases of various plants, it can also improve plant nutrient utilization efficiency , Promote plant growth (rice, corn, melon, etc.), enhance plant stress resistance and repair the environment polluted by agrochemicals, and play an increasingly important role in the development of sustainable agriculture. At present, more than 60 countries on five continents are using biological agents and bio-organic fertilizers containing Trichoderma components, which have become the most popular type of bio-control fungi. At the same time of popularization an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/01C12N1/14C12Q1/04C12R1/885
CPCC12N1/14C12N15/01C12Q1/04
Inventor 张屹梁志怀何英魏林肖姬玲朱菲莹田程
Owner HUNAN AGRI BIOTECH RES CENT
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