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Research method for protective mechanism of learning and memorizing function

A technology of learning memory and protection mechanism, which is applied in the field of medical research and can solve the problems that there are still few studies on autophagy function

Inactive Publication Date: 2019-03-08
XUZHOU MEDICAL UNIV
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Problems solved by technology

However, the mechanism of how β-amyloid plaques are reduced when neurons are excited has not been reported. Current studies believe that autophagy can clear β-amyloid or tau fibril tangles to improve AD, while There is little research on other functions of autophagy

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  • Research method for protective mechanism of learning and memorizing function
  • Research method for protective mechanism of learning and memorizing function
  • Research method for protective mechanism of learning and memorizing function

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Embodiment Construction

[0086] The technical solutions of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0087] 1. Materials and methods

[0088] 1.1 Materials

[0089] 1.1.1 Experimental animals

[0090] Wild-type clean-grade male ICR mice, gestational day 18 (E18) ICR mice, db / db diabetic and app / ps1 double transgenic mice were provided by the Institute of Model Animals, Nanjing University, license number: SCXK (Su) 2014 -001, SCXK (Su) 2015-001. Raised under SPF conditions, constant temperature and humidity in the room, 12h / 12h light / dark cycle alternating and free access to food and water.

[0091] 1.1.2 Main experimental reagents

[0092] Tris base; (B0016K011000, biosharp)

[0093] 30% Acr-Bis (29:1); (KGR0004-500, KG Bio, Jiangsu)

[0094] 1mol / L Tris, PH8.8; (KGB009CS, KGI, Jiangsu)

[0095] 1mol / L Tris, PH6.8; (KGB005, KGI, Jiangsu)

[0096] Sodium dodecyl sulfate SDS; (EC0227, Enjing Bio, Nanjin...

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Abstract

The invention discloses a research method for a protective mechanism of learning and memorizing functions. The research method comprises the steps that ICR male mice are divided into groups randomly to detect the effects of learning and memorizing of the mice in different stages and different time in the classic space recognition memory experiment, and the autophagy expression of synaptosome of the mice in the cerebral cortex is detected; then gamma-aminobutyric acidreceptor antagonist dicentrine and autophagy inhibitor 6-amino-3-methyl purine are used for separating cultured ICR mouse original generation nerve and intracerebroventricular injection of the ICR mice respectively, the learning and memorizing situation of the mice is detected by using Morris water maze and conditioned fear experiment, the expression of autophagy protein and PSD protein of the cerebral cortex of the mice and the change of synaptosome morphology are detected through immunohistochemistry, and then a regulatory mechanism between NMDA receptor subtypes and the autophagy process is observed.

Description

technical field [0001] The invention belongs to the technical field of medical research, and in particular relates to a research method for the protection mechanism of learning and memory functions. Background technique [0002] In recent years, with increasing life expectancy, the number of age-related diseases has increased, especially in elderly patients with dementia. Epidemiological surveys show that Alzheimer's disease (AD) is the most common cause of senile dementia, and AD cases will further increase until the middle of this century, becoming a major social and personal problem that affects the quality of human life . AD affects approximately 47 million people worldwide, a number that is expected to double within the next 20 years. The total cost of prevention and control was estimated at more than US$800 billion in 2010 and is expected to reach US$1 trillion globally by 2018. [0003] AD is a complex disease characterized by progressive cognitive impairment. How...

Claims

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Application Information

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IPC IPC(8): A01K67/02C12N5/0793C12Q1/32G01N33/533
CPCA01K67/02C12N5/0619C12Q1/32G01N33/533G01N2333/904
Inventor 张洋武煜
Owner XUZHOU MEDICAL UNIV
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