Biological surfactant bacterial strain production CTAB (cetyltrimethyl ammonium bromide) screening method

A technology of biological surface and active agent, applied in the field of petroleum microorganisms, can solve the problems of few high-efficiency strains, not very abundant, etc.

Inactive Publication Date: 2019-03-05
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the biosurfactant-producing bacteria screened at home and abroad are not very rich, and there are relatively few high-efficiency strains.

Method used

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  • Biological surfactant bacterial strain production CTAB (cetyltrimethyl ammonium bromide) screening method
  • Biological surfactant bacterial strain production CTAB (cetyltrimethyl ammonium bromide) screening method
  • Biological surfactant bacterial strain production CTAB (cetyltrimethyl ammonium bromide) screening method

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Embodiment 1

[0041] This example is used to illustrate the method of the present invention for efficiently separating and screening biosurfactant-producing bacteria from oil well production fluid in a certain block of Jiangsu Oilfield.

[0042] In the embodiment, the biosurfactant activator formula is produced: soybean oil 20g / L, glycerol 1g / L, NaNO 3 4g / L, Na 2 HPO 4 1g / L, KH 2 PO 4 1g / L, MgSO 4 0.2g / L, NaMoO 4 2H 2 O 0.05g / L.

[0043] CTAB plate is prepared according to the following formula: glycerol 20g / L, NaNO 3 2g / L, CaCl 2 2H 2 O 0.1g / L, Na 2 HPO 4 0.9g / L, KH 2 PO 4 0.7g / L, MgSO 4 ·7H 2 O 0.4g / L, agar 20g / L, trace elements 2mL; the composition of trace elements in the CTAB plate was prepared according to the following formula: FeSO 4 ·7H 2 O 2g / L, CTAB 0.2g / L, methylene blue 0.005g / L.

[0044] The preparation method of the double screening system is as follows: soybean oil 10g / L, glycerin 1g / L, NaNO 3 4g / L, Na 2 HPO 4 0.5g / L, KH 2 PO 4 0.5g / L, MgSO 4...

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Abstract

The invention belongs to the technical field of petroleum microorganism and particularly relates to a biological surfactant bacterial strain production CTAB screening method. The biological surfactantbacterial strain production CTAB screening method comprises the following steps of (1) sampling including selecting bacteria-containing water samples; (2) enrichment culture including mixing biological surfactant production activator with the bacteria-containing water samples in a sealed mode, performing shake culture to obtain an enriched bacteria solution; (3) CTAB plate separation including diluting the enriched bacteria solution, applying the diluted enriched bacteria solution onto a CTAB-containing plate for culture until dark blue halo-like bacterial colonies are formed onto the CTAB-containing plate, and inoculating single colonies inside the dark blue halo of the CTAB plate into a culture medium for shake culture; (4) strain secondary screening and capability measurement includingscreening out alternative high-yield biological surfactant strains; (5) stability verification to obtain high-yield biological surfactant strains. The biological surfactant bacterial strain production CTAB screening method is high in efficiency.

Description

technical field [0001] The invention belongs to the technical field of petroleum microbes, and more specifically relates to a method for using CTAB to screen biosurfactant-producing bacterial strains. Background technique [0002] Biosurfactants are a class of amphiphilic compounds synthesized by microorganisms. Their structures are both hydrophilic and hydrophobic, and they have high surface activity and biological activity. Biosurfactants include glycolipids, lipopeptides, lipoproteins, phospholipids, and neutral lipid derivatives. [0003] Compared with chemical surfactants, biosurfactants not only have excellent surface activity, chemical stability and thermal stability, but also have biological properties such as antibacterial, antiviral, environmentally friendly and degradable. It has been widely used in the fields of petroleum degradation, enhanced oil recovery, and bioremediation of heavy oil-contaminated soil. At present, foreign countries are mainly conducting re...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02
CPCC12N1/02C12N1/20
Inventor 齐义彬吕成远伦增珉王海涛马涛
Owner CHINA PETROLEUM & CHEM CORP
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