Cell Proliferation and Toxicity Assay Kit
A technology for toxicity detection and cell proliferation, which is applied in the field of cell biology, can solve problems such as uncontrollable reaction time, leakage or overfilling of detection solution, and no reaction termination reagent, etc., to achieve a stable storage environment, save time, and have little impact on cells Effect
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Embodiment 1
[0017] 1. The preparation method of 100 usages of the Cell Proliferation and Toxicity Detection Kit:
[0018] Preparation of detection solution: Weigh 8.6 mg sodium chloride, 0.3 mg potassium chloride, 0.28 mg calcium chloride and fully dissolve in 1ml ultrapure water, then add 7.8 mg WST-8 and 0.138 mg 2-methyl- 1,4-Naphthoquinone, fully dissolved and sterilized through a 0.22μm filter membrane, packed in a sterilized 1.5 ml Keli brown tube.
[0019] 2. Preparation of reaction termination solution: Add 8.5 μl HCl to 991.5 μl ultrapure water, mix well, pass through a 0.22 μm filter membrane to sterilize, and put it in a 1.5 ml standable transparent tube.
[0020] 3. The above two reagents pass the sterility test and are packed in the kit at a ratio of 1:1, which constitutes the cell proliferation and toxicity detection kit.
[0021] The method for counting C6 cells, detecting cell activity and proliferation using the kit of the embodiment of the present invention:
[0022] 1...
Embodiment 2
[0028] The preparation method of 100 usages of the cell proliferation and toxicity detection kit:
[0029] 1. Preparation of test solution: Weigh 8.6 mg of sodium chloride, 0.3 mg of potassium chloride, and 0.28 mg of calcium chloride and dissolve them in 1 ml of ultrapure water, then add 7.2 mg of WST-8 and 0.12 mg of 2- Methyl-1,4-naphthoquinone, fully dissolved, sterilized through a 0.22 μm filter membrane, and dispensed into sterilized 1.5 ml standable brown tubes.
[0030] 2. Preparation of reaction termination solution: Add 9 μl HCl to 991 μl ultrapure water, mix well, pass through a 0.22 μm filter membrane to sterilize, and dispense into 1.5 ml clear tubes.
[0031] 3. The above two reagents have passed the sterility test and are packed in the kit at a ratio of 1:1 to form a cell proliferation and toxicity detection kit.
[0032] Use the embodiment 2 kit of the present invention to carry out the toxicity detection method of paclitaxel to Hela cell:
[0033] 1. Prepare...
Embodiment 3
[0045] The preparation method of 500 usages of the Cell Proliferation and Toxicity Detection Kit:
[0046] 1. Preparation of test solution: Weigh 43 mg of sodium chloride, 1.5 mg of potassium chloride, and 1.4 mg of calcium chloride and dissolve them in 5ml of ultrapure water, then add 33 mg of WST-8 and 0.52 mg of 2-formazan Base-1,4-naphthoquinone, fully dissolved, sterilized through a 0.22μm filter membrane, and dispensed into sterilized 8 ml brown liquid bottles.
[0047] 2. Preparation of reaction termination solution: Add 42.5 μl HCl into 4.96 ml ultrapure water, mix well, pass through a 0.22 μm filter membrane to sterilize, and dispense into 8 ml natural color liquid bottles.
[0048] 3. The above two reagents pass the sterility test and are packed in the kit at a ratio of 1:1, which constitutes the cell proliferation and toxicity detection kit.
[0049] The method for detecting suspension cell activity and proliferation using the kit of Example 3 of the present invent...
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