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Lymphoma gene capture chip and applications thereof

A gene capture and lymphoma technology, which is applied in the field of lymphoma gene capture chip and detection of gene mutation of lymphoma genes, which can solve the problems of poor reproducibility of immunohistochemical methods and imperfect substitution of gene expression profiles.

Pending Publication Date: 2019-02-26
BGI GENOMICS CO LTD +2
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Several immunohistochemistry-based algorithms have been developed for the examination of paraffin-embedded tissue samples, which are in fact an imperfect surrogate for gene expression profiling and suffer from poor reproducibility of immunohistochemical methods

Method used

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  • Lymphoma gene capture chip and applications thereof
  • Lymphoma gene capture chip and applications thereof
  • Lymphoma gene capture chip and applications thereof

Examples

Experimental program
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Effect test

Embodiment

[0120] The following specific implementation process takes the tumor tissue (sample 1) of a lymphoma patient and the peripheral blood plasma (sample 2) of a lymphoma patient as the research objects, and the contents are as follows:

[0121] (1) Separation of peripheral blood samples

[0122] 1. Collect 1-2 tubes (5mL / tube) of peripheral blood from the subject into EDTA anticoagulant tubes, gently invert up and down (to prevent cell rupture) 6-8 times and mix well, and perform the following within 4-6 hours on the day of blood collection deal with;

[0123] 2. Centrifuge at 1600g for 10 minutes at 4°C. After centrifugation, divide the supernatant (plasma) into multiple 1.5mL / 2mL centrifuge tubes, and the middle layer of white blood cells cannot be absorbed during the absorption process;

[0124] 3. Centrifuge at 16,000g for 10 minutes at 4°C to remove residual cells, and transfer the supernatant (plasma) to a new 1.5mL / 2mL centrifuge tube. If the white blood cells cannot be su...

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Abstract

The invention proposes a lymphoma gene capture chip, which comprises: a substrate and a probe, wherein the probe is arranged on the substrate, and specifically recognizes at least one of genes represented by table 1. According to the present invention, the chip comprises the related Driver Genes, the high frequency mutation genes, the important genes in five cancer-related signaling pathways, thetargeted drug-sensitive and chemotherapeutic drug-sensitive genes and the drug-resistant related genes in various subtypes of common highly-incident lymphoma, can be used for the detection of the single-nucleotide site variation (SNV), the Indel insertion or deletion (Indel), the gene copy number variation (CNV) and the chromosome structural variation (SV) of lymphoma-associated genes, can achievethe detection of various subtypes of lymphoma, and can be effectively used for the early screening, the assisted diagnosis, the medication guidance and the recurrence monitoring of lymphoma.

Description

technical field [0001] The present invention relates to the field of biological detection, specifically, the present invention relates to a lymphoma gene trap chip and its application, and more specifically, the present invention relates to a lymphoma gene trap chip and a method for detecting gene variation of lymphoma gene. Background technique [0002] Lymphoma is a malignant tumor originating from the lymphatic hematopoietic system. It is mainly manifested as painless lymphadenopathy, hepatosplenomegaly, and various tissues and organs of the whole body can be involved, accompanied by systemic symptoms such as fever, night sweats, weight loss, and itching. There are about 350,000 new patients every year in the world, and about 25,000 new cases in my country every year. Malignant lymphoma is one of the fastest-growing malignant tumors in my country, with an annual increase of 5%, ranking the eighth among common malignant tumors. According to the origin of different lymphoc...

Claims

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Application Information

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IPC IPC(8): C40B40/06C40B50/06
CPCC12Q1/6869C40B50/06C12Q2535/122C12Q2565/501C12Q2531/113
Inventor 杨颖蔡宇航陈希王春丽叶明芝倪培相
Owner BGI GENOMICS CO LTD
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