Preparation method and application of a kind of mycoplasma avium culture medium and mycoplasma avium bacterial liquid

Abstract

The invention relates to a culture medium for avian mycoplasma, a preparation method for avian mycoplasma bacterial liquid and an application thereof, belonging to the field of biotechnology. The mycoplasma avium culture medium of the present invention is made up of basal culture medium and auxiliary component, and described basal culture medium is conventional liquid culture medium of mycoplasma avium, and auxiliary component is gelatin, Tween 80 and calcium chloride; Mycoplasma septicum or Mycoplasma gallinarum is inoculated, cultivated and harvested with bacterial liquid to produce Mycoplasma avium bacterial liquid to obtain Mycoplasma avium vaccine. The preparation method of the invention increases the antigen content of the mycoplasma avium bacterial liquid, simplifies the production process, reduces the production cost, reduces the occurrence probability of vaccine immunization side effects, and improves the vaccine immunization effect.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a preparation method of a mycoplasma avium culture medium, a mycoplasma avium bacterial liquid and an application thereof. Background technique [0002] Avian Mycoplasmosis (Avian Mycoplasmosis, AM) is an infectious disease of poultry caused by Mycoplasma avium. The common clinical pathogens are Mycoplasma Gallisepticum (MG) and Mycoplasma Synoviae (MS). Mycoplasma gallisepticum infection can cause Chronic Respiratory Disease (CRD), air sacculitis and sinusitis in poultry; Mycoplasma gallisepticum infection can cause symptoms such as joint swelling, synovial bursa and tendon inflammation, and parenchymal organ enlargement; Effective vaccine immunization is the main means of preventing and controlling the disease. [0003] At present, the production of mycoplasma avium vaccine mainly uses improved Frey's medium, CM2 medium, etc. to cultivate, and these mediums have a higher s...

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Problems solved by technology

[0003] At present, the production of mycoplasma avium vaccine mainly uses improved Frey's medium, CM2 medium, etc. to cultivate, and these mediums have a higher serum concentration (generally more than 10%), and the concentration of the cultured bacteria solution is not high (generally 10%). 8-10 CCU / ml), the strains need to be reproduced for each production, the production operation is cumbersome, the cost is high, the quality of the vaccine product is unstable (antigen content and purity are not high), and side reactions are prone to occur

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