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Construction method and detection method of UPLC characteristic chromatogram of stemona tuberosa medicinal material

A technology of Ye Baibu and its construction method, applied in the field of medicine, can solve the problems of not being able to fully reflect the quality, difficult to fully reflect the quality characteristics of Ye Baibu, unable to reflect the material basic characteristics of traditional Chinese medicine decoction, etc., so as to overcome the durability deviation, Ensuring high-quality, characteristic results

Active Publication Date: 2019-02-22
GUANGDONG YIFANG PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The 2015 edition of "Chinese Pharmacopoeia" has no content determination items, which cannot fully reflect its quality
The current literature research reports on the establishment of the characteristic map of Baibu, all of which are only for the raw materials of medicinal materials, and the index components are mostly fat-soluble components as the research objects, which are mainly used for qualitative identification of authenticity, origin, and quality differences of Chinese medicinal materials, which is difficult to fully reflect The quality characteristics of Ye Baibu cannot reflect the material basis characteristics of traditional Chinese medicine decoctions

Method used

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  • Construction method and detection method of UPLC characteristic chromatogram of stemona tuberosa medicinal material
  • Construction method and detection method of UPLC characteristic chromatogram of stemona tuberosa medicinal material
  • Construction method and detection method of UPLC characteristic chromatogram of stemona tuberosa medicinal material

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1 Construction of the UPLC characteristic map of the Herb Alba

[0069] 1. Instruments, reagents and reagents

[0070] See Table 1 for instruments, Table 2 for reagents, and Table 3 for reagents.

[0071] Table 1

[0072]

[0073] Table 2

[0074]

[0075]

[0076] table 3

[0077]

[0078] 2. Source of medicinal materials: In this study, 15 batches of raw medicinal materials were collected, including 6 batches from Yichang, Hubei, 3 batches from Jingmen, Hubei, and 6 batches from Dazhou, Sichuan. Detailed source: slightly. The details are shown in Table 4:

[0079] Table 4

[0080]

[0081] Remarks: The content of chlorogenic acid is determined by a self-designed content determination method.

[0082] 3. Preparation of reference substance reference solution, reference medicinal material reference solution and standard decoction

[0083] (1) Take chlorogenic acid reference substance: take an appropriate amount of chlorogenic acid reference...

Embodiment 2

[0200] Embodiment 2 is to the detection method of Herba Abaculi medicinal material

[0201] Stemona sessilifolia (Miq.) Miq., Stemona japonica (Bl.) Miq. or Stemona tuberose Lour. are dry tuberous roots of plants of the family Liceaceae. The present embodiment provides a kind of identification method to the Radix Albae medicinal material, and its steps are as follows:

[0202] (1) The chromatographic conditions are the same as the above 4(1).

[0203] (2) Preparation of the sample solution to be tested

[0204] The sample to be tested is the reference medical material purchased from the China Calibration Research Institute to purchase the Baibu chinensis medicinal material. Take 0.25g of the above-mentioned Baibu medicinal material powder, accurately weigh it, put it in a 10ml volumetric flask, add an appropriate amount of 80% (volume fraction) methanol aqueous solution, and ultrasonically (power 500W, frequency 40kHz) for 10min, take the extract, let the extract cool down, ...

Embodiment 4

[0208] Embodiment 4 is to the detection method of Rhizoma radiata medicinal material

[0209] The present embodiment provides a kind of method for the detection of the medicinal material of Rhizoma chinensis, the steps are as follows:

[0210] (1) The chromatographic conditions are the same as the above 4(1).

[0211] (2) Preparation of the sample solution to be tested

[0212]The samples to be tested are 9 batches of medicinal materials (BB01B1~BB06B1, BB16B1~BB18B1). Take 0.25g of the above 9 batches of medicinal material powders, weigh them accurately, put them in a 10ml volumetric flask, add an appropriate amount of 80% (volume fraction) methanol aqueous solution, and ultrasonically ( Power 500W, frequency 40kHz) for 10min, take the extract, let the extract cool, use 80% (volume fraction) methanol aqueous solution to set the volume to the mark, shake well, centrifuge (8000rpm, 3min), get the supernatant, The supernatant was filtered with a 0.22um microporous membrane, an...

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Abstract

The invention relates to a construction method and a detection method of a UPLC characteristic chromatogram of a stemona tuberosa medicinal material. The construction method of the characteristic chromatogram comprises the following steps: preparing a reference substance solution of a control sample from chlorogenic acid, neochlorogenic acid and cryptochlorogenic acid as a control sample respectively; preparing a reference substance solution of a control medicinal material from a stemona tuberosa control medicinal material; taking a stemona tuberosa medicinal material and a standard decoctionsample respectively to prepare a test sample solution and a standard decoction test sample solution; sucking the reference substance solution, the test sample solution and the standard decoction testsample solution respectively, and injecting the solutions into an ultra-high performance liquid chromatograph for measurement; and comparing a test sample chromatogram of the stemona tuberosa medicinal material with a test sample chromatogram of a standard decoction sample and calibrating characteristic peaks of 8 water-soluble components to obtain the UPLC characteristic chromatogram of the stemona tuberosa medicinal material. The characteristic chromatogram can be used for qualitative and quantitative analysis of the quality of the stemona tuberosa medicinal material, can ensure the qualityof a stemona tuberosa traditional decoction prepared from the medicinal material, and is also suitable for detecting other preparations containing stemona tuberosa.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a construction method and a detection method for the UPLC characteristic map of the medicinal material Abaca japonicus. Background technique [0002] Stemona sessilifolia Miq., Stemona japonica Miq. or Stemona tuberosa Lour. are dry tuberous roots of Stemona sessilifolia Miq., Stemona sessilifolia Miq. Excavated in spring and autumn, removed fibrous roots, washed, scalded in boiling water or steamed until there is no white heart, taken out, and dried in the sun. Baibu has the functions of moisturizing the lungs, lowering qi, relieving cough and killing insects. It is an important medicine for treating tuberculosis. It can not only inhibit tuberculosis bacillus and kill tuberculosis worms, but also reduce phlegm and relieve cough, nourish yin and moisten lungs, and make lung yin sufficient , the chemical source is beneficial, and the lungs can restore the Xuansu function. It is ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/36
CPCG01N30/02G01N30/36
Inventor 朱德全彭劲源谭吉娇张文芳林碧珊赵淑晶陈向东
Owner GUANGDONG YIFANG PHARMA
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