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Cell capture and fluorescence enhancement device and method based on biological lens

A fluorescence enhancement and lens technology, applied in the field of optics, can solve the problems that up-conversion fluorescent nanoparticles cannot achieve high biocompatibility, high efficiency, high precision and high flexibility, expensive devices, complex structures, etc. Small limitations, improved luminous efficiency, and small volume effects

Inactive Publication Date: 2019-02-22
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a device for cell capture and fluorescence enhancement based on biological microlenses, which solves the problems of high price, complex structure and cumbersome processing in the prior art.
[0006] Another object of the present invention is to provide a method for cell capture and fluorescence enhancement based on biological microlenses, which solves the problem in the prior art that high biocompatibility and high efficiency cannot be achieved while improving the luminous efficiency of upconversion fluorescent nanoparticles. , high precision and high flexibility issues

Method used

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  • Cell capture and fluorescence enhancement device and method based on biological lens
  • Cell capture and fluorescence enhancement device and method based on biological lens
  • Cell capture and fluorescence enhancement device and method based on biological lens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] The method for cell capture and fluorescence enhancement based on a biolens is specifically performed according to the following steps:

[0070] Step S1: Prepare an optical fiber probe 12 for capturing experimental objects and detecting signals. The optical fiber probe 12 is prepared from a standard multimode optical fiber through the fusion tapered method, specifically according to the following steps:

[0071] Step S11, after stripping off a 2 cm coating layer in the middle of the multimode optical fiber with optical fiber wire strippers, insert it into the capillary glass tube 11 to protect the multimode optical fiber. The inner diameter of the capillary glass tube 11 is 0.9 mm, and the wall 0.1 mm thick and 12 cm long;

[0072] Step S12, place the bare multimode optical fiber in parallel on the outer flame above the alcohol lamp, let it stand for 1.5 minutes until the optical fiber is melted, and draw the melted part with the help of hands at a speed of 6 mm per sec...

Embodiment 2

[0083] The method for cell capture and fluorescence enhancement based on a biolens is specifically performed according to the following steps:

[0084] Step S1: Prepare an optical fiber probe 12 for capturing experimental objects and detecting signals. The optical fiber probe 12 is prepared from a standard multimode optical fiber through the fusion tapered method, specifically according to the following steps:

[0085] Step S11, after stripping off a 2 cm coating layer in the middle of the multimode optical fiber with optical fiber wire strippers, insert it into the capillary glass tube 11 to protect the multimode optical fiber. The inner diameter of the capillary glass tube 11 is 0.9 mm, and the wall 0.1 mm thick and 12 cm long;

[0086] Step S12, place the bare multimode optical fiber in parallel on the outer flame above the alcohol lamp, let it stand for 1.5 minutes until the optical fiber is melted, and draw the melted part with the help of hands at a speed of 6 mm per sec...

Embodiment 3

[0094] The method for cell capture and fluorescence enhancement based on a biolens is specifically performed according to the following steps:

[0095] Step S1: Prepare an optical fiber probe 12 for capturing experimental objects and detecting signals. The optical fiber probe 12 is prepared from a standard multimode optical fiber through the fusion tapered method, specifically according to the following steps:

[0096] Step S11, after stripping off a 2 cm coating layer in the middle of the multimode optical fiber with optical fiber wire strippers, insert it into the capillary glass tube 11 to protect the multimode optical fiber. The inner diameter of the capillary glass tube 11 is 0.9 mm, and the wall 0.1 mm thick and 12 cm long;

[0097] Step S12, place the bare multimode optical fiber in parallel on the outer flame above the alcohol lamp, let it stand for 1.5 minutes until the optical fiber is melted, and draw the melted part with the help of hands at a speed of 6 mm per sec...

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Abstract

The invention discloses a cell capture and fluorescence enhancement device and method based on a biological lens. The device comprises a laser device, a photoelectric detector, an oscilloscope, an optical fiber coupler, a spectrograph, an optical coupling part, a microscope, an objective lens, a computer, an optical fiber adjusting rack, capillary glass tubes, an optical probe, a suspension, an objective table and a glass slide. The method includes following steps: S1, preparing the optical fiber probe for capturing an experimental object and a detection signal; S2, preparing a bacterial suspension; S3, preparing target capture cells; S4, preparing a biological lens suspension. Nano photon jet flow having stronger focusing property is utilized to excite stronger fluorescence of upconversion nano light particles, so that the light-emitting efficiency of the device is improved by two magnitude orders.

Description

technical field [0001] The invention belongs to the field of optical technology, in particular to a device and method for capturing cells and enhancing fluorescence based on biological microlenses. Background technique [0002] Up-conversion fluorescent nanoparticles can convert near-infrared laser light into visible light, and have excellent physical and chemical properties. They are widely used in bioimaging and biosensing. However, due to the limitations of their excitation and emission cross-sections, high luminous efficiency cannot be achieved. [0003] Several methods exist to increase the luminescence intensity of upconverting fluorescent nanoparticles, such as using nanotools such as silver or gold nanoparticles, plasmonic nanoantennas, metallic nanohole matrices, and photonic crystal resonators, by applying an external electromagnetic field. Modification generates a localized light field under resonance conditions to achieve higher excitation of upconversion fluores...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N23/2251G01N23/2202
CPCG01N21/6428G01N23/2202G01N23/2251
Inventor 李宇超李宝军张垚陈熙熙辛洪宝杨先光
Owner JINAN UNIVERSITY
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