Method for extracting kaempferol galactoside compounds from Nanshan tea

A technology of galactoside and kaempferol, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve problems such as insufficient research, and achieve the effect of simple extraction method and high extraction purity

Active Publication Date: 2020-12-25
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, not enough research has been done on the specific components in the extract

Method used

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  • Method for extracting kaempferol galactoside compounds from Nanshan tea
  • Method for extracting kaempferol galactoside compounds from Nanshan tea
  • Method for extracting kaempferol galactoside compounds from Nanshan tea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The tea fruit of Nanshan tea is dried in the air, the shell is removed, and the cake produced after the oil is removed by physical pressing is dried in the air, and properly crushed to obtain Nanshan tea tea cake. Ultrasonic extraction with 50% ethanol aqueous solution at 60°C for 3 times, 2 hours each time, with a solid-liquid ratio of 1:4 (m / v), and then filtered and combined to obtain the extract. Then the extract was concentrated, extracted with an equal volume of n-butanol to obtain an extract, and then diluted 20 times with water to obtain a sample liquid.

[0048] Shelling and degreasing are to maximize the extraction yield and reduce interference during the separation process. Appropriate raw material handling methods are also beneficial to avoid the loss of target compounds.

[0049] Choose the model of macroporous resin to be D101 type, first soak D101 macroporous resin with ethanol, then pack column (column volume BV 1 10L), rinse with ethanol and water in ...

Embodiment 2

[0055] The tea fruit of Nanshan tea is dried in the air, the shell is removed, and the cake produced after the oil is removed by physical pressing is dried in the air, and properly crushed to obtain Nanshan tea tea cake. Ultrasonic leaching with 60% ethanol aqueous solution at 70°C twice, each time for 2 hours, with a solid-liquid ratio of 1:2 (m / v), then filtered and combined to obtain the extract. Then the extract was concentrated, extracted with an equal volume of n-butanol to obtain an extract, and then diluted 22 times with water to obtain a sample liquid.

[0056] Choose the model of macroporous resin to be D101 type, first soak D101 macroporous resin with ethanol, then pack column (column volume BV 1 10L), rinse with ethanol and water in turn until there is no ethanol smell, and it can be detected with an alcohol meter, and the reading is less than 5. Slowly add the sample solution into the macroporous resin chromatography column, and then sequentially use 6 times of B...

Embodiment 3

[0061] The tea fruit of Nanshan tea is dried in the air, the shell is removed, and the cake produced after the oil is removed by physical pressing is dried in the air, and properly crushed to obtain Nanshan tea tea cake. Ultrasonic leaching with 55% ethanol aqueous solution at 65°C for 4 times, 2 hours each time, with a solid-liquid ratio of 1:4 (m / v), then filtered and combined to obtain the extract. Then the extract was concentrated, extracted with an equal volume of n-butanol to obtain an extract, and then diluted 18 times with water to obtain a sample liquid.

[0062] Choose the model of macroporous resin to be D101 type, first soak D101 macroporous resin with ethanol, then pack column (column volume BV 1 10L), rinse with ethanol and water in turn until there is no ethanol smell, and it can be detected with an alcohol meter, and the reading is less than 5. Slowly add the sample solution into the macroporous resin chromatography column, and then sequentially use 6 times of...

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Abstract

The invention provides a method for extracting a kaempferol galactoside compound from camellia semiserrata Chi. The method includes the steps that a camellia semiserrata Chi cake is subjected to leaching to obtain a sample solution; the sample solution is loaded with a wet method to a macroporous resin chromatographic column for gradient elution, and eluent is collected and then concentrated and freeze-dried to obtain a crude component; the crude component is loaded to a sephadex chromatographic column for gradient elution, and eluent is collected and then concentrated and freeze-dried to obtain a crude extract; the crude extract is dissolved by a first solvent and then subjected to sample injection, DAC preparation is carried out, and a flow segment containing the target compound is collected and then concentrated and freeze-dried to obtain crude isolate; the crude isolate is dissolved by a second solvent and then subjected to sample injection, isocratic elution is carried out by using an LC3000 efficient liquid chromatograph and a C18HCE chromatographic column with an acetonitrile-acetic acid aqueous solution as a mobile phase, and a flow segment containing the target compound iscollected and then concentrated and freeze-dried to obtain the target compound. By means of the method, the target compound is effectively separated from camellia plants for the first time, and the extraction purity is high.

Description

technical field [0001] The invention relates to the field of compound extraction, in particular to a method for extracting kaempferol galactoside compounds from Nanshan tea. Background technique [0002] Nanshan tea, also known as Guangning safflower oil tea (Camellia semiserrata Chi.), belongs to the plant of the genus Camellia (Camellia L.) of the family Theaceae (Theaceae), and is a perennial tree or shrub. Nanshan tea is mainly distributed in the southeast of Guangxi and the west of Guangdong in China. It is an important cultivated species of camellia oleifera. After research and comparison, it is found that among all kinds of Camellia oleifera, the chemical components in Guangning safflower Camellia oleifera are relatively rich. [0003] Nanshan tea and its cakes are especially rich in compounds such as flavonoids and saponins with various biological activities. At present, the research on Camellia oleifera cake mainly focuses on the optimization of various extractio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H1/08C07H17/07
Inventor 曹清明张晓帆宋钰蓉周文化钟海雁
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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