Method for testing growth of fungal hyphae in plant stems

A technology for mycelial growth and testing methods, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., and can solve the problems of lack of testing methods

Inactive Publication Date: 2019-02-19
南京胜跃新材料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the prior art also lacks a test method f

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: Mimic Fusarium causes head blight on wheat stalks:

[0019] (1) Take a glass tube with a diameter of 25mm, and open a vent hole every 3mm on the side wall of the glass tube, and control the diameter of the vent hole at 0.2mm;

[0020] (2) seal the bottom of the glass tube with a filter screen, the diameter of the filter hole of the filter screen is 0.25 mm, put the bottom of the glass tube into a petri dish, and pour culture solution into the petri dish;

[0021] (3) Pour the glass beads with a diameter of 0.5 mm into the glass tube;

[0022] (4) Pour the transparent nutrient solution inoculated with Fusarium into the glass tube full of glass beads, pour once every 20h, and pour 2 times continuously;

[0023] (5) Put the glass tube together with the culture dish into the culture room for 12 days;

[0024] (6) Observe and record the growth of Fusarium mycelium in the glass tube, and obtain the growth pattern of mycelium inside the wheat stalk when Fusariu...

Embodiment 2

[0025] Embodiment 2: Simulate the syringa fungus to cause syringa disease on the stalk of fescue grass:

[0026] (1) Take a glass tube with a diameter of 10mm, and open a vent hole every 3mm on the side wall of the glass tube, and control the diameter of the vent hole at 0.1mm;

[0027] (2) Seal the bottom of the glass tube with a filter screen, the diameter of the filter hole of the filter screen is 0.2mm, put the bottom of the glass tube into a petri dish, and pour culture solution into the petri dish;

[0028] (3) Pour the glass beads with a diameter of 0.4mm into the glass tube;

[0029] (4) pour into the glass tube that is full of glass beads the transparent nutrient solution that is inoculated with the mycelium of the cymbidium mycelium, pour once every 20h, continuously pour 2 times;

[0030] (5) Put the glass tube together with the culture dish into the culture room for 7 days;

[0031] (6) Observing and recording the growth of the mycelium of the fungus in the glass...

Embodiment 3

[0032] Embodiment 3: Simulate the cylindrical fungus to cause the cylindrical disease in Swangrass:

[0033] (1) Get a glass tube with a diameter of 50mm, and open a vent hole every 5mm on the side wall of the glass tube, and control the diameter of the vent hole at 0.2mm;

[0034] (2) Seal the bottom of the glass tube with a filter screen, the diameter of the filter hole of the filter screen is 0.3 mm, put the bottom of the glass tube into a petri dish, and pour culture solution into the petri dish;

[0035] (3) Pour the glass beads with a diameter of 0.5 mm into the glass tube;

[0036] (4) pour into the glass tube that is full of glass beads the transparent nutrient solution that is inoculated with the mycelium of Cystella cylindrica, topple over once every 30h, continuously topple over 2 times;

[0037] (5) Put the glass tube together with the culture dish into the culture room for 20 days;

[0038] (6) Observing and recording the growth of the mycelium of the fungus in ...

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PUM

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Abstract

The invention discloses a method for testing the growth of fungal hyphae in plant stems. The method includes the following steps: (1) taking a glass tube with a diameter of 10-50 mm, forming ventilation holes at intervals of 3-5 mm on the side wall of the glass tube, and controlling the diameter of the ventilation holes to be less than 0.2 mm; (2) sealing the bottom of the glass tube with a filterscreen, wherein the diameter of filter holes of the filter screen is 0.2-0.3 mm, placing the bottom of the glass tube into a culture dish, and pouring culture liquid into the culture dish; (3) pouring glass beads with a diameter of 0.4-0.5 mm into the glass tube; (4) pouring transparent nutrient solution inoculated with the hyphae into the glass tube filled with the glass beads, pouring once every 20-30 hours, and continuously pouring 1-2 times; (5) placing the glass tube together with the culture dish into a culture chamber for culture for 7-20 days; (6) observing and recording the growth ofthe hyphae in the glass tube to obtain a growth mode of the hyphae. The method is used for researching the growth condition of the fungal hyphae when fungal pathogens cause stem mycosis of gramineousplants.

Description

technical field [0001] The invention relates to a plant stalk simulation test method, in particular to a test method for fungal mycelium growth in plant stalks. Background technique [0002] In scientific research, it is often necessary to simulate the growth of grass plant stems to understand the growth of fungal diseases in grass plant stems. [0003] However, the prior art still lacks a test method for studying the growth of fungal hyphae in plant stems. Contents of the invention [0004] Purpose of the invention: Aiming at the deficiencies and defects of the prior art, the present invention provides a method for testing the growth of fungal hyphae in plant stems when fungal pathogens cause mycotic diseases of gramineous plant stems. . [0005] Technical solution: A method for testing fungal hyphae growth in plant stems of the present invention is characterized in that it includes the following steps: [0006] (1) Take a glass tube with a diameter of 10 mm to 50 mm, ...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
CPCC12Q1/02
Inventor 孙嘉斌孙晓哲魏秀慧
Owner 南京胜跃新材料科技有限公司
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