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Method for improving bagasse enzymolysis efficiency through delignification pretreatment and addition of additive

A technology of enzymatic hydrolysis efficiency and pretreatment, applied in fermentation and other directions, can solve the problems of increasing the cost of glucose production, long enzymatic hydrolysis time, etc., and achieve the effect of shortening enzymatic hydrolysis time, improving enzymatic hydrolysis efficiency and improving efficiency.

Pending Publication Date: 2019-02-19
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although different pretreatment methods can improve the subsequent enzymatic hydrolysis efficiency to a certain extent, to obtain higher enzymatic hydrolysis efficiency usually requires higher cellulase dosage and longer enzymatic hydrolysis time, which increases the production cost of glucose, so There is an urgent need to find a glucose production method that can improve enzymatic hydrolysis efficiency and reduce costs

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  • Method for improving bagasse enzymolysis efficiency through delignification pretreatment and addition of additive
  • Method for improving bagasse enzymolysis efficiency through delignification pretreatment and addition of additive

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Weigh 50g of absolutely dry bagasse raw material into a triangular flask, add 500ml of distilled water and 5g of NaOH, place it on a constant temperature shaker, set the speed to 150 rpm, and react for 12 hours at room temperature 25℃. After finishing, immediately The pretreatment residue is separated by vacuum filtration. The analysis of the pretreatment residue showed that the removal rates of hemicellulose and lignin reached 31.6% and 10.6%, respectively, and their effects on the glucose yield in the enzymatic hydrolysis section were as follows figure 1 Shown.

[0028] Take 5 grams (calculated on the absolute dry basis) of the pretreatment residue, add 100 FPU cellulase (Saili second generation), 0.75 grams Tween 80 and 250 mL acetic acid-sodium acetate buffer solution with pH=4.8 for enzymatic hydrolysis. During the enzymolysis process, the temperature is controlled at 50°C and the rotation speed is 150 rpm. After 24 hours of enzymatic hydrolysis, 1 mL of enzymatic h...

Embodiment 2

[0030] Weigh 50g of absolutely dry bagasse raw material and place it in a triangular flask, add 500ml of distilled water and 5g of NaOH, place it on a constant temperature shaker at a speed of 150 rpm, heat it to quickly increase the temperature to 60℃ and start timing, wait for the reaction When the time reaches 4 hours, the heating is stopped, and the reaction is immediately reduced to room temperature with condensed water, and the pretreatment residue is separated by vacuum filtration. The analysis of the pretreatment residue shows that the removal rates of hemicellulose and lignin reached 36.1% and 24.2%, respectively, and their influence on the glucose yield in the enzymatic hydrolysis section is as follows figure 1 Shown.

[0031] Take 5 grams (calculated on the absolute dry basis) of the pretreatment residue, add 100 FPU cellulase (Saili second generation), 0.75 grams Tween 80 and 250 mL acetic acid-sodium acetate buffer solution with pH=4.8 for enzymatic hydrolysis. Duri...

Embodiment 3

[0033] Weigh 50g of absolutely dry bagasse material and place it in a closed reactor equipped with a thermocouple, add 500ml of distilled water and 5g of NaOH, turn on the stirring, heat to quickly increase the temperature to 120°C and start timing, wait until the reaction time reaches 2 hours , Stop heating, and immediately lower the reaction to room temperature with condensed water, and use vacuum filtration to separate the pretreatment residue. The analysis of the pretreatment residue showed that the removal rates of hemicellulose and lignin reached 46% and 40.1%, respectively, and their effects on the glucose yield in the enzymatic hydrolysis section were as follows: figure 1 Shown.

[0034] Take 5 grams (calculated on the absolute dry basis) of the pretreatment residue, add 100 FPU cellulase (Saili second generation), 0.75 grams Tween 80 and 250 mL acetic acid-sodium acetate buffer solution with pH=4.8 for enzymatic hydrolysis. During the enzymolysis process, the temperatur...

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Abstract

The invention provides a method for improving the bagasse enzymolysis efficiency through delignification pretreatment and addition of an additive. The method comprises the steps of delignification pretreatment of a bagasse raw material, enzymolysis and the like. The air-dried and smashed bagasse raw material is mixed with a sodium hydroxide solution with a certain concentration or an acid sodium chlorite solution with a certain concentration, a reaction is conducted at the temperature of 20-180 DEG C for 0.5-12 hours for pretreatment, and pretreated residues rich in cellulose are obtained; cellulase and the additive are added into the residues for enzymolysis, so that the enzymolysis efficiency is improved. According to the method, through sodium hydroxide or sodium hypochlorite pretreatment and addition of the additive in the enzymolysis stage, the enzymolysis efficiency is improved, the enzymolysis time is shortened, the glucose production cost is reduced, and the method has a good application prospect.

Description

Technical field [0001] The invention relates to a method for efficient conversion and utilization of biomass, in particular to a method for bagasse raw materials to be pretreated by delignification and additives to improve the efficiency of bagasse enzymatic hydrolysis. Background technique [0002] As energy shortages and environmental issues continue to emerge, biomass as a renewable resource that can be converted into liquid fuels is receiving increasing attention. Among the abundant biomass resources on the earth, the liquid fuel made from lignocellulosic biomass has attracted the most attention. [0003] The method of making liquid fuel from lignocellulosic biomass is to hydrolyze the hemicellulose and cellulose in the raw material into monosaccharides such as xylose and glucose, produce ethanol through fermentation, or synthesize liquid fuel such as fuel oil through catalysis. However, because the cellulose in the biomass raw material is closely connected with hemicellulose ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
CPCC12P19/02C12P19/14C12P2201/00C12P2203/00
Inventor 张红丹谢君
Owner SOUTH CHINA AGRI UNIV
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