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Culture medium used for microorganism solid flux fermentation and preparation method and application thereof

A culture medium and microorganism technology, which is applied to the medium and preparation field of microbial solid-state flux fermentation, can solve the problems of small loading, affecting the use efficiency of a solid-state flux culture device, affecting the application efficiency of a solid-state flux culture device, and the like, Achieve easy molding, improve high-throughput screening efficiency, and reduce the risk of bacterial contamination

Active Publication Date: 2019-02-19
GUANGDONG HAITIAN INNOVATION TECH CO LTD +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in the follow-up long-term application, the inventor found that the aseptic subpackage operation of the multi-well plate medium during the solid-state culture of filamentous fungi seriously affects the application efficiency of the solid-state flux culture device
Since each well of the multi-well plate is connected as a whole, each well needs to be weighed separately, and the loading capacity of the well is also small, so it is necessary to be very careful in the process of subpackaging the medium to ensure that each well is subpackaged and cultivated. The consistency of the base and the prevention of contamination of bacteria, the operation of this aspect greatly affects the efficiency of the solid-state flux culture device

Method used

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  • Culture medium used for microorganism solid flux fermentation and preparation method and application thereof
  • Culture medium used for microorganism solid flux fermentation and preparation method and application thereof
  • Culture medium used for microorganism solid flux fermentation and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～5

[0036] The culture media used for microbial solid-state flux fermentation in Examples 1 to 5 are all disc-shaped, and the components of the culture media and the weight percentages of each component in the culture media are shown in Table 1. Among them, the fineness of bran, soybean meal and wheat is 40-80 mesh, and the fineness of cornstarch and maltodextrin powder is more than 80 mesh; the quality of each medium is 0.4-0.9g, and the diameter of the medium is 6-15mm, and the thickness of the culture medium is 5-10mm.

[0037] Embodiments 1-5 are used for the preparation method of the culture medium of microbial solid-state flux fermentation as follows:

[0038] (1) Dry each component in the culture medium until the water content in each component is not higher than 10%; at the same time, grind the bran, soybean meal and wheat to a fineness of 40-80 mesh, cornstarch and malt The dextrin powder is pulverized to a fineness of more than 80 mesh.

[0039] (2) Weighing each compo...

Embodiment 6

[0043] The impact of different raw material ratios of embodiment 6 on the culture medium becoming sheet

[0044] In this example, according to the preparation method of the culture medium in Examples 1-5, the culture medium was prepared by using different ratios of raw materials, and the influence of the ratio of raw materials on the formation of the culture medium was compared. The results are shown in Table 2.

[0045] From formula No. 1 in Table 2, it can be seen that using bran, soybean meal, and wheat alone as raw materials for medium tableting cannot be formed, so it is necessary to add a certain filler to assist in forming. However, in order not to affect the effect of the filler on the components of the solid-state fermentation medium, corn starch is selected as the preferred filler. However, as can be seen from the analysis results of No. 2-6 medium from the back, adding 1% cornstarch can improve the tableting of the medium, but 50% of the tableting medium cannot be m...

Embodiment 7

[0051] The application of embodiment 7 substratum of the present invention in Aspergillus oryzae

[0052] 1. Porous fermentation device

[0053] Refer to the solid-state flux culture device described in the patent CN 107446804A.

[0054] 2. Experimental materials and methods

[0055] 2.1. Culture medium composition and production method

[0056] The composition of medium 1 is composed of the following raw materials by weight percentage: 23% of bran; 56% of soybean meal; 21% of wheat.

[0057] The medium 2 is composed of the following raw materials by weight percentage: bran 25%; soybean meal 55%; wheat 20%.

[0058] The composition of medium 3 is composed of the following raw materials by weight percentage: 22% of bran; 58% of soybean meal; 20% of wheat.

[0059] The composition of medium 4 is composed of the following raw materials by weight percentage: 22% of bran; 55% of soybean meal; 23% of wheat.

[0060] The medium 5 is composed of the following raw materials by wei...

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Abstract

The invention relates to a culture medium used for microorganism solid flux fermentation and a preparation method and application thereof, and belongs to the field of solid culturing fermentation of industrial fermentation microorganisms. The cultural medium used for microorganism solid flux fermentation is in a piece shape and is prepared from, by weight, 10%-82.5% of bran, 10%-80% of bean pulp,5%-70% of wheat, 1%-5% of corn starch and 0.5%-1.5% of maltodextrin powder. In the solid flux culturing process, the culture medium can be directly put in different holes of a pore plate for subsequent treatment, the packaging speed of the culture medium in different holes can be ensured, the consistence of the weight of the culture medium can be ensured, and therefore the higher application efficiency of a solid flux culturing device is achieved; in addition, the culture medium is easy to form, cannot be broken easily, and is good in disintegration effect and small in error among culture mediums of the same batch.

Description

technical field [0001] The invention relates to a culture medium and its preparation method and application, in particular to a culture medium for microbial solid-state flux fermentation and its preparation method and application. Background technique [0002] Solid-state fermentation is a common way for industrial fermentation microorganisms to carry out industrial production of enzyme preparations and secondary metabolites. At present, the research on solid-state fermentation of industrial fermentation microorganisms mainly focuses on substrate optimization and strain selection. Substrate optimization is to optimize and transform the solid-state fermentation process to improve product quality; strain selection is to conduct natural, chemical, and physical mutations on production strains to obtain products that can be improved in solid-state fermentation. Target strains with excellent traits of quality. However, due to the randomness of strain mutation and the cumbersome ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/20C12N1/16C12R1/69C12R1/685C12R1/785C12R1/845C12R1/125C12R1/85
Inventor 周斌肖桂龙王静童星
Owner GUANGDONG HAITIAN INNOVATION TECH CO LTD
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