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Method for detecting ketoconazole based on SERS of nano-silver colloid

A technology for surface Raman enhancement and detection of ketoconazole, applied in the detection field, can solve the problems of long time, high cost, complicated pretreatment, etc., and achieve the effects of good effect, uniform particle and high sensitivity

Active Publication Date: 2019-02-15
FUJIAN ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to overcome the problems of high cost, complicated pretreatment, and long time consumption in the existing ketoconazole detection, the purpose of the present invention is to provide a rapid detection method for trace ketoconazole, which is easy to operate, high in sensitivity, and can save a lot of time. Advantages of economy and time cost

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  • Method for detecting ketoconazole based on SERS of nano-silver colloid

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Embodiment 1

[0034] A method based on nano-silver colloid surface Raman enhanced detection of ketoconazole, the method may further comprise the steps:

[0035] (1) Pretreatment of copper sheets: Cut the copper sheets into 2cm×5cm rectangular small copper sheets, soak them in acetone, ethanol and ultrapure water for 10 minutes, and finally soak the copper sheets in 0.1 mol / L HNO 3 Remove the oxide film on the surface; the above-mentioned treated copper sheet is rinsed with ultrapure water and dried at room temperature;

[0036] (2) Nano-silver plating on copper sheet; copper sheet in 0.02 mol / L SnCl 2 and 0.02 mol / L hydrochloric acid mixed solution for 3 minutes, after drying at room temperature; the copper sheet in 0.01 mol / L AgNO 3 Soak in a mixed solution with a mass fraction of 0.3% polyvinylpyrrolidone for 5 minutes, and dry at room temperature; repeat the above two steps for four cycles, and finally put the substrate in SnCl 2 Soak in the solution for 3 minutes, rinse with ultrapure...

Embodiment 2

[0048] A method based on nano-silver colloid surface Raman enhanced detection of ketoconazole, the method may further comprise the steps:

[0049] (1) Pretreatment of copper sheets: Cut the copper sheets into 2cm×5cm rectangular small copper sheets, soak them in acetone, ethanol and ultrapure water for 10 minutes, and finally soak the copper sheets in 0.1 mol / L HNO 3 Remove the oxide film on the surface; the above-mentioned treated copper sheet is rinsed with ultrapure water and dried at room temperature;

[0050] (2) Nano-silver plating on copper sheet; copper sheet in 0.03 mol / L SnCl 2 and 0.03 mol / L hydrochloric acid mixed solution for 6min, after room temperature drying; 3 Soak in a mixed solution with a mass fraction of 0.3% polyvinylpyrrolidone for 5 minutes, and dry at room temperature; repeat the above two steps for four cycles, and finally put the substrate in SnCl 2 Soak in the solution for 3 minutes, rinse with ultrapure water;

[0051] (3) Grafted mercapto compo...

Embodiment 3

[0062] A method based on nano-silver colloid surface Raman enhanced detection of ketoconazole, the method may further comprise the steps:

[0063] (1) Pretreatment of copper sheets: Cut the copper sheets into 2cm×5cm rectangular small copper sheets, soak them in acetone, ethanol and ultrapure water for 10 minutes, and finally soak the copper sheets in 0.1 mol / L HNO 3 Remove the oxide film on the surface; the above-mentioned treated copper sheet is rinsed with ultrapure water and dried at room temperature;

[0064] (2) Nano-silver plating on the copper sheet; the copper sheet was coated with 0.04 mol / L SnCl 2 and 0.05 mol / L hydrochloric acid mixed solution for 8min, after drying at room temperature; copper sheet in 0.03 mol / L AgNO 3 Soak in a mixed solution with a mass fraction of 0.3% polyvinylpyrrolidone for 5 minutes, and dry at room temperature; repeat the above two steps for four cycles, and finally put the substrate in SnCl 2 Soak in the solution for 3 minutes, rinse wi...

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Abstract

The invention discloses a method for detecting ketoconazole based on the SERS of nano-silver colloid, wherein a hydrophobic substrate prepared by a grafted thiol compound is used as a material. The method comprises the following steps of: mixing the ketoconazole solution of different concentrations with the nano-silver colloid, carrying out centrifugal separation, and testing by a laser Raman spectrometer to obtain the SERS spectrum of the ketoconazole; acquiring a relationship between the Raman spectrum absorption intensity and the concentration of the ketoconazole of a certain wave number, and establishing a mathematical relationship between the Raman spectrum absorption intensity and the concentration of the ketoconazole; and using the laser Raman spectrometer to test a ketoconazole solution of unknown concentration, and calculating the concentration of the ketoconazole according to the mathematical relationship between the Raman spectrum absorbance intensity and the concentration of the ketoconazole of the certain wave number. The method for detecting ketoconazole based on the SERS of nano-silver colloid solves the problems that the cost of ketoconazole detection is high, a sample needs to be separated in multiple steps, and the like, has the advantages of being easy to operate, high in sensitivity, and capable of saving a lot of economic and time cost, and has wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a method for detecting ketoconazole based on nano-silver colloid surface Raman enhancement. Background technique [0002] Ketoconazole is an antifungal drug that has inhibitory effects on dermatophytes such as Trichophyton, Epidermophyton, Microsporum and yeasts such as Candida. However, ketoconazole has the risk of serious hepatotoxicity, so it can only be used when other effective antifungal treatments are considered, and local treatment is ineffective due to factors such as its infection site, area, and depth. [0003] The traditional methods for the detection of antibacterial agent residues mainly include: mass spectrometry, capillary electrophoresis, chromatographic analysis, immunoassay, etc. These methods are highly reliable, but they are time-consuming, and due to the complicated pre-treatment process of samples, etc., resulting in analysis The cost is rela...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 柯金炼苏良佺常颖萃刘晓辉陈志强
Owner FUJIAN ACAD OF FORESTRY
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