Combined detection test strip for heavy metal and creatinine, and preparation method and application thereof

Abstract

The invention discloses a combined detection test strip for heavy metal and creatinine, and a preparation method and an application thereof. The invention aims to provide a rapid, accurate, simple, and inexpensive test strip for simultaneously detecting the heavy metal ions and the creatinine. The technical points of the invention is that: the detection test strip comprises a bottom plate, which is sequentially connected with a sample pad, a bonding pad, a chromatographic membrane, a creatinine test paper, and an absorbent pad. The chromatographic membrane is provided with a C-line and a T-line. The bonding pad is sprayed with a heavy metal ion antibody labeled with the same colloidal gold particle. The chromatographic membrane is coated with a heavy metal ion antigen labeled with fluorescent microspheres at T-line position and a BSA labeled with the fluorescent microspheres at the C-line position. The invention relates to the field of immunochromatography detection of fluorescence anddry chemical analysis.

Description

technical field [0001] The invention relates to the fields of fluorescence immunochromatography detection and dry chemical analysis, in particular to a test strip for combined detection of heavy metals and creatinine. Background technique [0002] The original meaning of heavy metals refers to metals with a specific gravity greater than 5, such as mercury, gold, silver, copper, iron, zinc, lead, etc. Although some heavy metals are trace elements needed by individuals, most of the heavy metals are not needed by the body, and all heavy metals accumulate in the body to a certain extent, which will cause chronic poisoning. Some heavy metals entering the body will destroy the normal physiological functions of the human body. They are called toxic heavy metals, such as chromium (Cr), cadmium (Cd), mercury (Hg), lead (Pb), arsenic (As), etc. [0003] Heavy metal pollution refers to environmental pollution caused by heavy metals and their compounds. Heavy metal pollution includes:...

AI Technical Summary

Problems solved by technology

[0006] There are many detection methods for cadmium, such as: atomic absorption method, spectrophotometry, atomic fluorescence spectrometry, inductively coupled plasma mass spectrometry, high performance liquid chromatography, etc. Most of them require expensive instruments, complicated operations and long detection time, and requires specialized technicians to complete

[0007] There are also many detection methods for creatinine, such as: alkaline picric acid method, enzymatic method, capillary electrophoresis method, liquid chromatography method, etc. Most of them also require related equipment, complicated operation, slow detection speed, expensive detection cost, and clinical Inconvenient to use and cannot be calibrated with simultaneous detection of cadmium

[0008] Therefore, the existing urine cadmium detection technology is difficult to meet the needs of rapid detection in vitro, it is necessary to invent a method that can simultaneously detect Cd 2+ and creatinine rapid diagnostic test strips, providing fast, accurate, simple and cheap practical promotion technology

[0009] Existing cadmium detection methods that meet the above requirements include lateral flow chromatography test strips, and creatinine detection methods include dry chemical test paper. The former is fluorescent immunochromatography technology, and the latter is dry chemical analysis technology. The color of colloidal gold may affect the observation of dry chemical test paper, and the colored substance of dry chemical test paper may affect the reaction of fluorescence immunochromatography, so it is necessary to study a method that can simultaneously detect urinary cadmium and creatinine

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