Preparation method of anglerfish skin peptides, and immunoloregulation application of prepared anglerfish skin peptides

A fish skin and Ankang technology, applied in the field of Ankang fish skin peptides, can solve the problems of inability to remove the fishy smell of marine fish, large molecular weight of peptides, unsuitable for Ankang fish skin peptides, etc., and achieve high fish skin utilization and high immune activity , the effect of uniform molecular weight

Active Publication Date: 2019-02-01
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The soft-shelled turtle peptide preparation method directly prepares polypeptides through enzymatic hydrolysis, and the obtained product has a high content of soft-shelled turtle protein, and the molecular weight of the polypeptide is relatively large. At the same time, this method cannot remove the fishy smell of marine fish, so it is not suitable for direct use in monkfish. Preparation of skin peptide

Method used

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  • Preparation method of anglerfish skin peptides, and immunoloregulation application of prepared anglerfish skin peptides

Examples

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Embodiment 1

[0030] A preparation method of monkfish skin peptide, comprising the following steps:

[0031] (1) Clean the fish skin scraps of the recovered monkfish, cut into pieces, then mix the fish skin with water, add vitamin E to disperse evenly, then add lipase to degrease at 30°C for 4 hours, and then add 25 wt% isopropyl Degrease the alcohol solution for 2 hours again, sieve the fish skin and rinse it with water until there is no smell of isopropanol, the amount of vitamin E added is 0.5wt% of the fish skin, the amount of lipase added is 1.6wt% of the fish skin, and the lipase activity is 30000U / g;

[0032] (2) Put the degreased fish skin into the water dispersed with high-temperature calcined mussel powder, immerse and deodorize, then sieve, rinse and recover the fish skin, wherein the high-temperature calcined mussel powder is made through the following process: after cleaning the mussel shells Grind it to 60-80 mesh, then mix it with sodium carbonate and silane coupling agent ...

Embodiment 2

[0044] A preparation method of monkfish skin peptide, comprising the following steps:

[0045] (1) Clean the recovered monkfish skin scraps, cut into pieces, then mix the fish skin with water, add vitamin E to disperse evenly, then add lipase to degrease at 38°C for 2 hours, then add isopropanol solution to degrease again 1h, sieve the fish skin and wash it with water until there is no smell of isopropanol, the amount of vitamin E added is 0.8wt% of the fish skin, the amount of lipase added is 1.2wt% of the fish skin, the lipase activity is 40000U / g, isopropyl Alcohol solution concentration 30wt%;

[0046] (2) Put the degreased fish skin into the water dispersed with high-temperature calcined mussel powder, immerse and deodorize, then sieve, rinse and recover the fish skin, wherein the high-temperature calcined mussel powder is made through the following process: after cleaning the mussel shells Grind it to 60-80 mesh, then mix it with sodium carbonate and silane coupling age...

Embodiment 3

[0053] A preparation method of monkfish skin peptide, comprising the following steps:

[0054] (1) Clean the reclaimed monkfish skin scraps, cut into pieces, then mix the fish skin with water, add vitamin E to disperse evenly, then add lipase to degrease 3 at 40°C, and then add 32wt% isopropanol The solution was degreased again for 3 hours, and the fish skin was sieved and rinsed with water until there was no smell of isopropanol. The amount of vitamin E added was 1 wt% of the fish skin, the amount of lipase added was 2 wt% of the fish skin, and the lipase activity was 50000U / g;

[0055] (2) Put the degreased fish skin into the water dispersed with high-temperature calcined mussel powder, immerse and deodorize, then sieve, rinse and recover the fish skin, wherein the high-temperature calcined mussel powder is made through the following process: after cleaning the mussel shells Grind it to 60-80 mesh, then mix it with sodium carbonate and silane coupling agent at a mass ratio o...

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Abstract

The invention relates to the technical field of anglerfish skin peptides, and provides a preparation method of the anglerfish skin peptides to solve the problems of large molecular weight and bitter and fishy taste of polypeptides prepared from marine fish. Anglerfish skins are degreased by cooperating lipase with isopropanol, high-temperature calcined mussel powder is used to deodorize the degreased anglerfish skins and synergistically remove the bitter taste of the immunoloregulation peptides, an acidic environment, a pulsed electric field and sodium borohydride or sodium borohydride acetatecooperate with each other to weaken the cross-linking degree of the fish skin proteins and enhance the swelling of the fish skin proteins, the fish skin proteins are separated and extracted by a protein extracting enzyme having a weak decomposition effect on the fish skin proteins, and the fish skin proteins are decomposed by a highly-specific protease to prepare the anglerfish skin peptides. Theproportion of the anglerfish skin peptides having a molecular weight of 1000-3500 Da is high; and compared with fish skin proteins, the fish skin peptides have the advantages of high yield reaching about 78 wt%, uniform molecular weight, no bitter or fishy taste, high fish skin utilization rate and high immune activity, and can be applied to immunoloregulation.

Description

technical field [0001] The invention relates to the technical field of monkfish skin polypeptides, in particular to a method for preparing monkfish skin peptides and the immunoregulatory application of the prepared monkfish skin peptides. Background technique [0002] Polypeptides with immunomodulatory activity can participate in the regulation of specific and non-specific immune functions of the body by affecting the growth and development of immune organs, the activity and function of immune cells, and the expression, synthesis and release of immune molecules, and play an important role in maintaining the normal and healthy immune function of the body. effect. Generally, the smaller the molecular weight of the polypeptide, the higher the immunomodulatory activity. At present, the acquisition of polypeptides with immunomodulatory activity relies on chemical synthesis, DNA recombinant expression, and extraction from biological tissues and organs. Among them, marine biologi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34A23L33/18
CPCA23V2002/00A23L33/18C07K1/34C12P21/06A23V2200/324A23V2250/55
Inventor 杨最素
Owner ZHEJIANG OCEAN UNIV
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