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Coating liquid containing atoxigenic aspergillus flavus and coating method and application thereof

A technology of Aspergillus flavus and coating liquid, applied in the directions of application, botanical equipment and methods, fungicides, etc., can solve economic animal and human harm, limited to laboratory research stage, no non-toxic Aspergillus flavus, etc. question

Inactive Publication Date: 2019-01-25
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aspergillus flavus is widely distributed and is susceptible to contamination by Aspergillus flavus during peanut planting, harvesting, transportation and storage; most Aspergillus flavus can produce aflatoxin during growth and reproduction; peanuts contaminated with aflatoxin Not only cause harm to economic animals and human body, but also cause waste of resources
[0003] At present, there are already many methods for aflatoxin detoxification, detoxification, and degradation; many microorganisms that inhibit the growth of Aspergillus flavus, inhibit the production of toxins by Aspergillus flavus and degrade aflatoxin have been isolated and cultivated; the non-toxic Aspergillus flavus is a It is an important microorganism that inhibits the growth, toxin production and degradation of aflatoxin of Aspergillus flavus; however, most of the non-toxigenic Aspergillus flavus that have been isolated are limited to the laboratory research stage, and there is no non-toxigenic Aspergillus flavus that has been used in actual production check in

Method used

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  • Coating liquid containing atoxigenic aspergillus flavus and coating method and application thereof
  • Coating liquid containing atoxigenic aspergillus flavus and coating method and application thereof
  • Coating liquid containing atoxigenic aspergillus flavus and coating method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 The preparation of non-toxin-producing Aspergillus flavus spores

[0031] The non-toxigenic Aspergillus flavus AS3.3950 (purchased from Shanghai Beinuo Biotechnology Co., Ltd.) was inoculated on Czapek's agar medium, cultured at 28°C for 5 days, and then the spores on the medium were dipped with cotton swabs in a sterile In 0.1% Tween-80, use an oscillator to oscillate evenly, and then use a hemocytometer to count and adjust its concentration to 10 11 cfu / mL spare.

Embodiment 2

[0032] Example 2 The inhibitory effect of bioflavonoids on the toxin production of Aspergillus flavus

[0033] The first group: toxin-producing Aspergillus flavus ATCC 28539 (purchased from Beijing Bei Nachuanglian Biotechnology Research Institute) was inoculated in the comprehensive PDA liquid medium (adding 0.3% proanthocyanidins); the final concentration was 10 6 cells / mL, cultured on a shaker at 28°C.

[0034] The second group: the toxigenic Aspergillus flavus ATCC 28539 is inoculated in the comprehensive PDA liquid medium (adding 0.3% rutin); its final concentration is 10 6 cells / mL, cultured on a shaker at 28°C.

[0035] The third group: the toxigenic Aspergillus flavus ATCC 28539 is inoculated in the comprehensive PDA liquid medium (adding 0.3% hesperetin); its final concentration is 10 6 cells / mL, cultured on a shaker at 28°C.

[0036] The fourth group: the toxigenic Aspergillus flavus ATCC 28539 was inoculated in the comprehensive PDA liquid medium; the final conce...

Embodiment 3

[0039] The impact of embodiment 3 proanthocyanidins addition amount on the toxin production of Aspergillus flavus

[0040] The toxigenic Aspergillus flavus ATCC 28539 (purchased from Beijing Bei Nachuanglian Biotechnology Research Institute) was inoculated respectively in 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.8%, 1.0 % proanthocyanidins in the comprehensive PDA liquid medium; to make the final concentration of 10 6 cells / mL, cultured on a shaker at 28°C for 6 days, and sampled for determination of aflatoxin B 1 , B 2 , G 1 , G 2 The content of each group was repeated 3 times. The result is as figure 2 Shown:

[0041] Depend on figure 2 It can be seen that with the increase of the amount of proanthocyanidins added, aflatoxin B 1 , B 2 , G 1 , G 2 When the content of proanthocyanidins is reduced, when the amount of proanthocyanidins added is greater than 0.5%, the reduction of aflatoxin is slow and tends to be stable; considering the principle of cost saving,...

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Abstract

The invention discloses coating liquid containing atoxigenic aspergillus flavus and a coating method and application thereof, and belongs to the technical field of food safety and processing. The coating liquid containing the atoxigenic aspergillus flavus, which is disclosed by the invention, is prepared by adding spores of the atoxigenic aspergillus flavus into coating agent solution; an additiveamount of the spores of the atoxigenic aspergillus flavus is 10<7> to 10<9> pieces per litre. 0.1 to 0.5% of bioflavonoid is added into the coating liquid to take a synergistic effect with the sporesof the atoxigenic aspergillus flavus. The coating liquid disclosed by the invention can effectively prevent pollution of aflatoxin to peanuts, wherein the spores of the atoxigenic aspergillus flavusand the bioflavonoid take the synergistic effect so as to improve the effect of preventing aflatoxin pollution.

Description

technical field [0001] The invention belongs to the technical field of food safety and processing, and in particular relates to a coating solution containing non-toxic aflatoxin, a coating method and application thereof. Background technique [0002] Peanut is an important oil crop and economic crop. The planting area of ​​peanut in my country is 5 million hectares, and the annual output of peanut is 14 million tons, accounting for more than 40% of the world's total peanut production. Aflatoxins are mainly secondary metabolites produced by Aspergillus flavus (A.flavus) and Aspergillus parasiticus (A.parasiticus), which are derivatives of dihydrofuranocoumarins, and were approved by the World Health Organization (WHO) in 1993. Cancer Research Institute delineated as a class I carcinogen. Aspergillus flavus is widely distributed and is susceptible to contamination by Aspergillus flavus during peanut planting, harvesting, transportation and storage; most Aspergillus flavus can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B9/14A23B9/26A01N63/04A01N43/16A01P3/00
CPCA01N43/16A23B9/14A23B9/26A23V2002/00A01N63/30A23V2250/2116A23V2250/21166A23V2250/5086A23V2250/511
Inventor 于春娣杨庆利唐娟赵海燕朱英莲
Owner QINGDAO AGRI UNIV
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