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A method for inducing regenerated plants of Chinese pepper with handle cotyledons

A technology for Chinese pepper and regeneration plants, which is applied in the field of plant tissue culture, can solve the problems of large influence on the regeneration system, difficulty in constructing the regeneration system, and influence on the genetic transformation of Chinese pepper, and achieve the effect of improving the survival rate and elongation induction rate

Active Publication Date: 2022-03-04
HAINAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the study of Chinese pepper, the genotype, explant type and hormone ratio have a great influence on the regeneration system, which makes the construction of the regeneration system more difficult, and further affects the genetic transformation of Chinese pepper

Method used

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  • A method for inducing regenerated plants of Chinese pepper with handle cotyledons
  • A method for inducing regenerated plants of Chinese pepper with handle cotyledons
  • A method for inducing regenerated plants of Chinese pepper with handle cotyledons

Examples

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Embodiment 1

[0028] A method for inducing regenerated plants of Chinese pepper with handle cotyledons, comprising the following steps:

[0029] (1) Acquisition of sterile seedlings: take the seeds of Chinese pepper and place them on a stainless steel filter, then put them into a culture bottle, add sterile water, and heat the culture bottle with sterile water in a water bath at 50°C After 20 minutes, carry out sterilization treatment with 70% ethanol shock for 2 minutes, then rinse with sterile water, then shake and shake with 3% sodium hypochlorite solution for 10 minutes, then rinse with sterile water for 3 times, and finally put the sterilized seeds in Blot the water on sterile filter paper, inoculate it on the MS medium without adding plant hormones, the MS medium is a medium containing agar and sucrose, and cultivate under light to obtain sterile seedlings, the light culture time is 14h, and the light culture The intensity is 2000lux.

[0030] (2) Induction of adventitious buds: Take...

Embodiment 2

[0035] A method for inducing regenerated plants of Chinese pepper with handle cotyledons, comprising the following steps:

[0036] (1) Acquisition of sterile seedlings: take the seeds of Chinese pepper and place them on a stainless steel filter, then put them into a culture bottle, add sterile water, and heat the culture bottle with sterile water in a water bath at 60°C After 40 minutes, perform sterilization treatment, first shake with 80% ethanol for 3 minutes, then rinse with sterile water, then shake and shake with 5% sodium hypochlorite solution for 20 minutes, then rinse with sterile water for 6 times, and finally sterilize the seeds Put it on sterile filter paper to absorb the water, inoculate it on the MS medium without adding plant hormones, the MS medium is the medium containing agar and sucrose, and culture under light to obtain sterile seedlings, the light culture time is 14-18h , and the intensity of light cultivation was 2500lux.

[0037] (2) Induction of advent...

Embodiment 3

[0042] A method for inducing regenerated plants of Chinese pepper with handle cotyledons, comprising the following steps:

[0043] (1) Acquisition of sterile seedlings: take the seeds of Chinese pepper and place them on a stainless steel filter, then put them into a culture bottle, add sterile water, and heat the culture bottle with sterile water in a water bath at 55°C After 30 minutes, carry out sterilization treatment, first shake with 75% ethanol for 1 minute, then rinse with sterile water, then shake and shake with 4% sodium hypochlorite solution for 15 minutes, then rinse with sterile water for 5 times, and finally sterilize the seeds Place on sterile filter paper to absorb moisture, inoculate on MS medium without adding phytohormone, the MS medium is a medium containing agar and sucrose,

[0044] Aseptic seedlings were obtained by light culture, the light culture time was 16 hours, and the light culture intensity was 2200 lux.

[0045] (2) Induction of adventitious bud...

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Abstract

The invention discloses a method for inducing regenerated plants of Chinese pepper with stipe cotyledons, which comprises the steps of obtaining sterile seedlings, inducing adventitious buds, elongating adventitious buds, rooting adventitious buds, transplanting and the like. The sterilized seeds were inoculated on the MS medium without adding phytohormones, and the cotyledons with stalks of suitable seedling-age sterile seedlings were selected as explants, and the adventitious bud induction medium obtained by the research was used to produce adventitious buds, so that the induction differentiation rate Improvement; using the elongation medium obtained in the research, the elongation induction rate of adventitious buds has been greatly improved; the rooting medium is conducive to the induction of adventitious roots, thereby improving the survival rate of regenerated plants. Using the petiolate cotyledons of Chinese pepper as explants, combined with the screened adventitious bud induction medium, adventitious bud elongation medium and rooting medium, significantly improved the bud induction rate and rooting rate, the in vitro regeneration system constructed by the present invention, It laid the foundation for the study of genetic transformation of Chinese pepper.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for inducing regenerated plants of Chinese pepper with stipe cotyledons. Background technique [0002] Tissue culture is an important part of agricultural biotechnology and plays an important role in the in vitro regeneration of vegetable crops. Capsicum is a plant of the genus Capsicum in the family Solanaceae, and it is one of the main vegetables in my country. There are 5 cultivated species of Capsicum: annual pepper (Capsicum annuum), Chinese pepper (Capsicum chinense), shrubby pepper (Capsicum frutescens), drooping pepper (Capsicum baccatum) and Capsicum pubescens. The characteristic of Chinese pepper is that the corolla is white, each node has more than 2 flowers, and the sepals are constricted. Most of the leaves and peels in the species are shrunken, and the fruit has a special aroma and is highly spicy (there are also a few varieties with low spicy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 汪志伟舒黄英郝园园周燕成善汉朱国鹏
Owner HAINAN UNIV
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