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Vanda tissue culture and rapid propagation culture medium

A technology of tissue culture rapid propagation and subculture culture medium, which is applied in the field of tissue culture rapid propagation medium of Vanda orchid, can solve the problems of no shoot tip differentiation tissue culture medium, unsatisfactory cultivation effect, and few researches, etc., and achieve improvement The effect of inducing differentiation rate, shortening the culture cycle and improving quality

Inactive Publication Date: 2018-02-02
秦素梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some scholars have studied the method of obtaining Vanda seedlings by using Vanda capsules or Vanda shoot tips as explants for tissue culture, but due to the lack of related research, no medium that is more suitable for the growth of shoot tip differentiation tissues has been found. , resulting in a long training cycle and unsatisfactory training results during the tissue culture process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A kind of Vanda orchid tissue culture rapid propagation medium, comprising induction medium, subculture medium, strong seedling medium, the specific composition of each medium is as follows:

[0026] Induction medium: 1 / 2MS+ diethyl hexanoate 1mg / L+ Moringa oleifera leaf juice 5.0g / L+ Lycium barbarum leaf juice 5.0g / L, pH 5.4;

[0027] Subculture medium: MS+ naphthalene acetic acid 2mg / L+ silver nitrate 1mg / L+ agar 5.0g / L+ Moringa oleifera leaf juice 5.0g / L+ wolfberry leaf juice 5.0g / L+ sucrose 2.0g / L, pH 5.4;

[0028] Strong seedling medium: 1 / 3MS+ activated carbon 1g / L+ indole butyric acid 0.8mg / L+ silver nitrate 1mg / L+ agar 5.0g / L+ Moringa oleifera leaf juice 5.0g / L+ wolfberry leaf juice 6.0g / L+ sucrose 2.0g / L , pH 5.4.

Embodiment 2

[0030] Induction medium: 1 / 2MS+ diethyl hexanoate 1.5mg / L+ Moringa oleifera leaf juice 6.0g / L+ Lycium barbarum leaf juice 4.0g / L, pH 5.5;

[0031] Subculture medium: MS+ naphthalene acetic acid 2.5mg / L+ silver nitrate 1.5mg / L+ agar 6.0g / L+ Moringa oleifera leaf juice 6.0g / L+ wolfberry leaf juice 4.0g / L+ sucrose 3.0g / L, pH 5.5;

[0032] Strong seedling medium: 1 / 3MS + activated carbon 1g / L + indole butyric acid 0.9mg / L + silver nitrate 1.5mg / L + agar 6.0g / L + Moringa leaf juice 6.0g / L + wolfberry leaf juice 5.0g / L + sucrose 3.0g / L, pH 5.5.

Embodiment 3

[0034] A kind of Vanda orchid tissue culture rapid propagation medium, comprising induction medium, subculture medium, strong seedling medium, the specific composition of each medium is as follows:

[0035] Induction medium: 1 / 2MS+ diethyl hexanoate 2mg / L+ Moringa leaf juice 8.0g / L+ Lycium barbarum leaf juice 3.0g / L, pH 5.6;

[0036] Subculture medium: MS+ naphthalene acetic acid 3mg / L+ silver nitrate 2mg / L+ agar 8.0g / L+ Moringa oleifera leaf juice 8.0g / L+ wolfberry leaf juice 3.0g / L+ sucrose 4.0g / L, pH 5.6;

[0037] Strong seedling medium: 1 / 3MS+ activated carbon 1g / L+ indole butyric acid 1.0mg / L+ silver nitrate 2mg / L+ agar 8.0g / L+ Moringa oleifera leaf juice 8.0g / L+ wolfberry leaf juice 4.0g / L+ sucrose 4.0g / L , pH 5.6.

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Abstract

The invention provides a vanda tissue culture and rapid propagation culture medium and belongs to the technical field of plant propagation. The culture medium disclosed by the invention is prepared from an induction culture medium, subculture culture medium and strong sprout culture medium. Horseradish tree leaf juice and folium lycii juice are added into culture media used in all the culture stages to serve as nutritional agents; a formula of the induction medium comprises 1 / 2MS + 1mg / L to 2mg / L of diethyl aminoethyl hexanoate + the 5.0g / L to 8.0g / L of horseradish tree leaf juice + 3.0g / L to5.0g / L of folium lycii juice; a pH value is 5.4 to 5.6. A formula of the culture medium disclosed by the invention can remarkably improve a differentiation rate, an explants growth coefficient and a rooting rate of vanda, shorten a culture period and improve quality of vanda tissue culture seedlings.

Description

【Technical field】 [0001] The invention relates to the technical field of plant propagation, in particular to a culture medium for rapid propagation of vanda orchids. 【Background technique】 [0002] Vanda Orchid, also known as Vanda Orchid, is a typical epiphytic orchid of the Orchidaceae Vanda Orchid genus. It is a tropical orchid with uniaxial stems. It has no pseudobulbs, has obvious stems, is firm and upright. The plants vary greatly, the height of small plants is about 30cm, and the height of large plants can reach more than 2m. There are many thick aerial roots on the stem, and the root length can reach more than 1m. The leaves are arranged in two rows on both sides of the stem, band-shaped or cylindrical, leathery, green. The leaf surface has a thicker cuticle, which has strong drought resistance. The racemes are extracted from the leaf axils, and there are 10 to 20 flowers, each flower can bloom for 2 to 3 weeks, and they bloom sequentially from bottom to top. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 秦素梅
Owner 秦素梅
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