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Seed propagation method of Vanda

A vanda seed technology, applied in the field of plant propagation, can solve the problems of not finding shoot tip differentiation tissue culture medium, unsatisfactory cultivation results, and few researches, so as to increase the induction differentiation rate, shorten the cultivation period, and improve the quality Effect

Inactive Publication Date: 2018-03-30
秦素梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some scholars have studied the method of obtaining Vanda seedlings by using Vanda capsules as explants for tissue culture, but due to the lack of relevant research, they have not found a medium that is more suitable for the growth of shoot tip differentiated tissues, resulting in the tissue culture process. Medium, the training cycle is long, and the training effect is not satisfactory

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A seed propagation method of Vanda orchid, including sterilization of explants, induction culture, subculture and strong seedling culture, the specific steps are:

[0023] (1) Disinfection of explants: Collect the pollinated capsules of Vanda orchid as explants, first rinse with running water, then soak in 75% alcohol for 1 min, and then use 0.1% HgCl 2 Disinfect the solution for 10 minutes, rinse with sterile water twice, cut off both ends of the fruit, cut the fruit, and use the seeds in the fruit for inoculation;

[0024] (2) Induction culture: Sprinkle the seeds in the fruit evenly on the induction medium, culture for 55 days at a temperature of 25±1°C, an illumination of 2000 LX, and an illumination of 10 hours a day to obtain Vanda orchid protocorm; The composition of the induction medium is: VW+diethyl aminoethyl hexanoate 1mg / L+moringa leaf juice 5.0g / L+ wolfberry leaf juice 5.0g / L;

[0025] (3) Subculture: Transform the Vanda orchid protocorm grown in step (2) into th...

Embodiment 2

[0028] A seed propagation method of Vanda orchid, including sterilization of explants, induction culture, subculture and strong seedling culture, the specific steps are:

[0029] (1) Disinfection of explants: Collect the pollinated capsules of Vanda orchid as explants, first rinse with running water, then soak in 75% alcohol for 1.5 minutes, and then use 0.1% HgCl 2 Disinfect the liquid for 12 minutes, rinse with sterile water 3 times, cut off both ends of the fruit, cut the fruit, and use the seeds in the fruit for inoculation;

[0030] (2) Induction culture: Sprinkle the seeds in the fruit evenly in the induction medium, the composition of the induction medium is: VW+diethyl aminoethyl hexanoate 1.5mg / L+moringa leaf juice 6.0g / L+ wolfberry leaf juice 4.0g / L; Cultivate for 50 days under the conditions of a temperature of 25±1℃, a light of 2000LX, and a light of 12h per day to obtain Vanda protocorm;

[0031] (3) Subculture: Transform the Vanda orchid protocorm grown in step (2) int...

Embodiment 3

[0034] A seed propagation method of Vanda orchid, including sterilization of explants, induction culture, subculture and strong seedling culture, the specific steps are:

[0035] (1) Disinfection of explants: Collect the pollinated capsules of Vanda orchid as explants, first rinse with running water, then soak in 75% alcohol for 2 minutes, and then use 0.1% HgCl 2 Disinfect the solution for 15 minutes, rinse with sterile water 4 times, cut off both ends of the fruit, cut the fruit, and use the seeds in the fruit for inoculation;

[0036] (2) Induction culture: Sprinkle the seeds in the fruit evenly on the induction medium, cultivate for 52 days under the conditions of a temperature of 25±1°C, an illumination of 2000 LX, and an illumination of 14 hours per day to obtain Vanda protocorm; The composition of the induction medium is: VW+diethyl aminoethyl hexanoate 2mg / L+moringa leaf juice 8.0g / L+ wolfberry leaf juice 3.0g / L;

[0037] (3) Subculture: Transform the Vanda orchid protocorm g...

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Abstract

The invention provides a seed propagation method of Vanda and belongs to the technical field of plant propagation. The seed propagation method adopts a Vanda capsule as an explant and comprises explant disinfection, induced culture, subculture, strong seedling culture and other steps. Moringa oleifera leaf juice and lycium barbarum leaf juice are added in a culture medium used in each of the culture stages as nutrients. The induced culture medium comprises VW, 1-2 mg / L of diethylaminoethyl hexanoate, 5.0-8.0 g / L of moringa oleifera leaf juice and 3.0-5.0 g / L of lycium barbarum leaf juice. Through the culture medium formula provided by the invention, the induced differentiation rate, the explant proliferation coefficient and the rooting rate of the Vanda can be significantly improved, the culture period is shortened, and the quality of Vanda tissue culture seedlings is improved.

Description

【Technical Field】 [0001] The invention relates to the technical field of plant propagation, in particular to a seed propagation method of Vanda orchid. 【Background technique】 [0002] Vanda orchid, also known as Vanda orchid, is a typical epiphytic orchid of the Orchidaceae Vanda orchid. It is a tropical orchid with uniaxial stems. It has no false bulbs, but has obvious stems and is firm and upright. Plants vary greatly, small plants are about 30cm tall, and large plants can reach more than 2m. There are many strong aerial roots on the stem, and the root length can reach more than 1m. The leaves are arranged in two rows on both sides of the stem, banded or cylindrical, leathery and green. The leaf surface has a thick cuticle, which has strong drought resistance. The raceme is drawn from the leaf axils, and there are 10-20 flowers, each flower can bloom for 2 to 3 weeks, and open sequentially from bottom to top. Vanda orchid flowers are huge, unrestrained, and rich in color. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 秦素梅
Owner 秦素梅
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