Method for rapid aided detection of micro-plastics in water environment samples by using Nile red dyeing

Abstract

A method for rapid aided detection of micro-plastics in water environment samples by using Nile red dyeing belongs to the field of environmental pollution control. The method comprises the following steps: firstly preparing a Nile red dye solution with Nile red and an acetone solution; preparing an ink-dyed organic film; next, before the dyeing, adding the working solution of the Nile red dye solution filtered by the organic film to the water sample to dye the water into a light blue color; filtering the dyed water sample by the organic film dyed with ink; finally, placing the filtered organicfilm in a culture dish, and uniformly coating glue on the filter to fix the substance on the filter for subsequent analysis and use. The method utilizes micro-plastics on a hydrophobic surface to adsorb Nile red dye solution, and observes the fluorescent substance on the filter by a fluorescence microscope at a wavelength of 450-490 nm to perform Raman detection. The method provided by the invention is simple and quick to prepare the dyeing agent, good in adsorption effect, and simple in dyeing method, and is not only short in time consumption, low in cost, but also can eliminate interferenceof some non-plastic materials, compared with the direct visual inspection method.

Description

technical field [0001] The invention belongs to the field of environmental pollution control and relates to a method for quickly assisting detection of microplastics in water environment samples by using Nile red dyeing. Background technique [0002] With the increase of global plastic production year by year, the problem of plastic pollution has been listed by the United Nations Environment Program as one of the eight major environmental problems in the world. The ensuing microplastic pollution problem has also attracted widespread attention from politicians and researchers around the world. Microplastics are plastic films, fibers, particles and fragments with an effective diameter of less than 5 mm. It can be generated by large plastics under environmental factors (light, heat, radiation, etc.), or it can be plastic microbeads added to facial detergents or toothpaste, and the latter is far more than the former. In the past ten years since the concept of microplastics was...

AI Technical Summary

Problems solved by technology

But Nile Red is a kind of solution-induced chromatic dye solution, which will leave epi-fluorescence on the film when passing through common membranes, masking the fluorescence of the material on the membrane, and at the same time, PC ( The high cost of polycarbonate) membrane and the movement of particles on the membrane are not conducive to the later observation and counting

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