Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Traditional Chinese medicine composition and application for inhibiting fae and faeg gene expression of enterotoxigenic Escherichia coli k88 pili

A technology of gene expression and composition, applied in the field of pharmaceutical compositions, can solve the problems of breeding production loss, endangering the health and growth and development of piglets, and intestinal damage of piglets, etc.

Active Publication Date: 2021-09-10
WUHAN HUAYANG ANIMAL PHARMA
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] After K88 pili fae, faeG gene and protein are normally expressed, the bacteria colonize and multiply on the intestinal epithelial cells of the animal, and produce enterotoxins, causing intestinal damage and diarrhea in piglets, endangering the health, growth and development of piglets, and causing huge losses to breeding production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Traditional Chinese medicine composition and application for inhibiting fae and faeg gene expression of enterotoxigenic Escherichia coli k88 pili
  • Traditional Chinese medicine composition and application for inhibiting fae and faeg gene expression of enterotoxigenic Escherichia coli k88 pili
  • Traditional Chinese medicine composition and application for inhibiting fae and faeg gene expression of enterotoxigenic Escherichia coli k88 pili

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0024] a. the preparation method of Cortex Phellodendri extract:

[0025] 1) Cortex Phellodendri is removed from impurities, washed, moistened thoroughly, sliced, and dried to obtain clean medicinal materials.

[0026] 2) Weigh in the weighing room according to the prescription feeding amount, crush the dried Cortex Phellodendron medicinal material into coarse powder, and put the crushed Phellodendron Phellodendron coarse powder into a turnover container;

[0027] 3) Soaking in acid water, adding the amount of medicinal materials required by the production instructions into the reaction kettle, adding 8 times the amount of 0.5% sulfuric acid aqueous solution to soak for 24 hours, filtering, then soaking in 6 times the amount of sulfuric acid aqueous solution for 24 hours, filtering, and then using 6 times the amount of sulfuric acid aqueous solution Soak in double the amount of sulfuric acid aqueous solution for 24 hours, filter for later use, combine the above two extracts, a...

Embodiment 1

[0042] Phellodendron phellodendron extract and pomegranate peel extract weight ratio 1:0 (cortex phellodendron extract alone) to obtain phellodendron phellodendron extract, respectively take 150mg and 300mg of phellodendron phellodendron extract, weigh them accurately, put them in a 100ml volumetric flask, add 80ml of PBS, Sonicate for 40 minutes, let cool, dilute to the mark with PBS, shake well, and filter for later use.

Embodiment 2

[0044] Phellodendron Phellodendri extract and pomegranate peel extract were mixed at a weight ratio of 1:1 to obtain Chinese medicinal composition 1. Take 1150 mg and 300 mg of traditional Chinese medicinal composition respectively, accurately weigh them, put them in a 100ml volumetric flask, add 80ml of PBS, and ultrasonically Treat for 40 minutes, let cool, dilute to volume with PBS, shake well, and filter for later use.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a traditional Chinese medicine composition for inhibiting the expression of fae and faeG genes of enterotoxigenic Escherichia coli K88 pilus and its application, which comprises Phellodendron Phellodendri extract and pomegranate peel extract. The invention utilizes natural plant extracts to inhibit the fae and faeG gene expression of enterotoxin-producing Escherichia coli pilus, reduce the colonization and reproduction of ETEC in intestinal epithelial cells of piglets, without affecting the colonization of beneficial microorganisms in the intestinal tract, and increase the intestinal tract The abundance of beneficial bacteria, while reducing the risk of diarrhea in piglets, ensures the diversity of intestinal flora; at the same time, the antioxidant substances in cork and pomegranate peels protect piglets from the intestinal oxidative damage caused by weaning stress; and antibiotics Compared with the traditional Chinese medicine composition, it is not easy to produce drug resistance, and it can overcome the side effects of antibiotics while killing harmful bacteria and reducing the abundance of beneficial bacteria, which is more conducive to the healthy breeding of pigs, and can be used as a clinical Feed additives are used for a long time.

Description

technical field [0001] The invention relates to a pharmaceutical composition, in particular to a traditional Chinese medicinal composition for inhibiting the expression of fae and faeG genes of enterotoxigenic Escherichia coli K88 pili and its application. Background technique [0002] Enterotoxigenic Escherichia coli (ETEC) belongs to the Enterobacteriaceae family, Escherichia genus, Escherichia coli species, and is the most common type of pathogenic Escherichia coli. ETEC is the main pathogenic bacteria causing diarrhea in piglets, and its pathogenicity depends on the ability to colonize the epithelial cells of the small intestine and the ability to produce enterotoxin. Enterotoxin is the direct cause of diarrhea. Only when Escherichia coli adheres to the epithelial cells of the small intestine can it avoid being cleared by intestinal peristalsis and intestinal fluid flushing, and then colonize and multiply in the host to secrete enterotoxin. The basis of ETEC's action is...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/756A61P1/00A61P31/04A61P1/12
CPCA61K36/185A61K36/756A61P1/00A61P1/12A61P31/04A61K2300/00
Inventor 马立保晏向华潘云鑫徐保阳晏依琴徐晓帆黄俊程
Owner WUHAN HUAYANG ANIMAL PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com