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Oxidase-like activity of nitrogen-doped carbon nanospheres and uses thereof

A nitrogen-doped nanocarbon and oxidase technology, which is applied in the medical application field of nanomaterials, can solve problems such as no reports of enzyme-like activity.

Active Publication Date: 2019-01-15
KUNSHAN XINYUNDA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] So far, there is no report on whether nitrogen-doped nanocarbon materials or nitrogen-doped nanocarbon spheres have enzyme-like activity

Method used

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  • Oxidase-like activity of nitrogen-doped carbon nanospheres and uses thereof
  • Oxidase-like activity of nitrogen-doped carbon nanospheres and uses thereof
  • Oxidase-like activity of nitrogen-doped carbon nanospheres and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1. Preparation and characterization of nitrogen-doped nano-carbon spheres (abbreviated as N-Carbon)

[0055] 1.798g of melamine, 2.1mL of formalin aqueous solution (37.0wt%) and 15mL of distilled water were mixed in a three-necked flask, and stirred evenly at 80°C to obtain a colorless transparent solution. Then, 0.6 g of phenol, 2.1 mL of formalin solution (37.0 wt%) and 15 mL of NaOH (0.1 mol·L -1 ) solution, stirred at 66° C. for 0.5 hour (h) to obtain low molecular weight phenolic resin (molecular weight less than 10,000 Daltons). Then 15 mL of block copolymer Pluronic F127 (0.7 g) was added to the reaction system, and after stirring at 66 °C for 2 h at a speed of 350 rpm, 50 mL of distilled water was added to the reaction solution to continue the reaction. During the reaction, the reaction solution turned from colorless to pink and finally dark red. After 24 hours of reaction, the reaction was terminated and a precipitate appeared. After standing stil...

Embodiment 2

[0058] Example 2.N-carbon has oxidase activity

[0059]The oxidase activity of N-carbon is determined by directly oxidizing the chromogenic substrate TMB in sodium acetate buffer and detecting the specific absorption peak of the oxidized TMB substrate at 652nm. The reaction system was as follows: 10 μL of TMB (final concentration of 0.416 mM) was added to 1.0 mL of reaction solution (0.1 M sodium acetate buffer, pH 4.25) containing different concentrations of N-carbon nanoparticles. After reacting at room temperature for 10 minutes (min), use a microplate reader (Bio-Rad) to measure the absorption peak of the reaction system at 652 nm to determine the enzyme activity and strength. In the experiment, Fe 3 o 4 Nanozyme and carbon nanosphere Carbon not doped with nitrogen were used as negative controls. Experiments have confirmed that Fe 3 o 4 Nanozymes do not have oxidase activity.

[0060] The result is as image 3 As shown, N-carbon shows typical oxidase activity, under...

Embodiment 3

[0061] Example 3.O 2 Effect on N-carbon oxidase activity

[0062] The oxidase reaction system is the same as in Example 2. Before the reaction, nitrogen (N 2 ) treatment group, the reaction system was treated with nitrogen bubbles for 40 min in advance, and then 2 Carry out oxidase reaction and measure under air environment; Air treatment group is with embodiment 2; Oxygen (O 2 ) treatment group, the reaction system was treated by bubbling with oxygen for 40 minutes in advance to saturate the reaction solution with oxygen, and then the oxidase reaction and measurement were carried out. Ultraviolet full-wavelength absorption was measured using Nanodrop2000 (Thermo Fisher).

[0063] The result is as Figure 4 As shown, after nitrogen treatment, in the case of low oxygen content, compared with the air condition, the whole reaction is greatly restricted; after the oxygen bubbling treatment, the oxygen content of the reaction system increases, which promotes the reaction compa...

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Abstract

The present invention relates to oxidase-like activity of nitrogen-doped carbon nanospheres and uses thereof, in particular to oxidase-like activity of nitrogen-doped carbon nanospheres and uses thereof in tumor treatment, anti-infection treatment and sewage treatment.

Description

technical field [0001] This application belongs to the interdisciplinary field of nano biomedicine, nano technology, tumor biology and the like. In particular, the application relates to medical applications of nanomaterials. More specifically, the present application relates to the oxidase-like activity of nitrogen-doped carbon nanospheres and their use in tumor treatment, anti-infection treatment and sewage treatment. Background technique [0002] The research on nitrogen-doped carbon nanomaterials (N-doped carbon nanomaterials) has become one of the hotspots in the field of international carbon materials. The nitrogen atom has one more valence electron than the carbon atom. After nitrogen doping into the carbon six-membered ring structure, nitrogen-containing functional groups such as pyridine, pyrrole, graphite nitrogen, and pyridine oxide can be formed, which can not only improve the surface chemical activity of nano-carbon materials, but also The electronic structure...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/00A61P35/00A61P31/00A61P31/04C02F3/34C02F101/32C02F101/30
CPCA61K33/00C01P2004/04C01P2004/32C01P2004/62C01P2004/64C02F3/342C02F2101/30C02F2101/322
Inventor 阎锡蕴范克龙高利增奚菊群王培霞范磊段德民
Owner KUNSHAN XINYUNDA BIOTECH CO LTD
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