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Nanoparticle liquid with oxidase activity and preparation method

A platinum nanoparticle, oxidase activity technology, applied in chemical instruments and methods, organic compound/hydride/coordination complex catalysts, chemical/physical processes, etc., to achieve the effect of simple method, high catalytic activity and short cycle

Inactive Publication Date: 2019-09-17
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the synthesis of platinum nanoparticle liquid with oxidase activity by using nucleotides

Method used

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  • Nanoparticle liquid with oxidase activity and preparation method
  • Nanoparticle liquid with oxidase activity and preparation method
  • Nanoparticle liquid with oxidase activity and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for preparing a platinum nanoparticle liquid with oxidase activity, comprising the steps of:

[0027] (1) Add 50 μL of 50 mM cytidine-5'-monophosphate disodium salt ethylene glycol solution into a 1.5 mL centrifuge tube;

[0028] (2) Add 50 μL of 50 mM potassium tetrachloroplatinate aqueous solution to the solution obtained in step (1), add ethylene glycol to make the total volume 500 μL, shake and mix, and stand at 60°C for 24 hours to obtain a solution with oxidase activity platinum nanoparticles liquid. Liquid (being called as CMP-Pt1.0 liquid), obtains its average particle diameter by transmission electron microscope scanning and is 3.3nm (see figure 1 ).

[0029] Determination of Oxidase Activity in Platinum Nanoparticle Liquid:

[0030] Take 20 μL of the platinum nanoparticle liquid with oxidase activity obtained in Example 1 in a 1.5mL centrifuge tube and add 980 μL of distilled water to dilute and mix evenly. In eight 4mL cuvettes, add 20 μL of the a...

Embodiment 2

[0033] A method for preparing a platinum nanoparticle liquid with oxidase activity, comprising the steps of:

[0034] (1) Add 100 μL of 5mM cytidine-5'-monophosphate disodium salt ethylene glycol solution into a 1.5mL centrifuge tube;

[0035] (2) Add 25 μL of 100 mM potassium tetrachloroplatinate aqueous solution to the solution obtained in step (1), add ethylene glycol to make the total volume 500 μL, shake and mix, and let stand at 20°C for 72 hours to obtain platinum nanoparticles liquid. The liquid (named as CMP-Pt0.2 liquid) has an average particle size of 3.1 nm obtained by scanning with a transmission electron microscope.

[0036] Determination method is the same as embodiment 1, to the K of 3,3',5,5'-tetramethylbenzidine m The value is 0.064mM.

Embodiment 3

[0038] A method for preparing a platinum nanoparticle liquid with oxidase activity, comprising the steps of:

[0039] (1) Add 50 μL of 250 mM cytidine-5'-monophosphate disodium salt ethylene glycol solution into a 1.5 mL centrifuge tube;

[0040] (2) Add 100 μL of 25 mM potassium tetrachloroplatinate aqueous solution to the solution obtained in step (1), add ethylene glycol to make the total volume 500 μL, shake and mix well, and let stand at 80°C for 12 hours to obtain a solution with oxidase activity platinum nanoparticles liquid. The liquid (named as CMP-Pt5.0 liquid) has an average particle size of 3.7 nm obtained by scanning with a transmission electron microscope.

[0041] Determination method is the same as embodiment 1, to the K of 3,3',5,5'-tetramethylbenzidine m The value is 0.048mM.

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Abstract

The invention discloses nanoparticle liquid with oxidase activity and a preparation method. The preparation method comprises the following steps: (1) adding cytidine-5'-monophosphate disodium salt ethylene glycol solution, guanosine-5'-monophosphate disodium salt ethylene glycol solution, adenosine-5'-monophosphate disodium salt ethylene glycol solution or thymidine-5'monophosphate disodium salt ethylene glycol solution into a centrifugal tube; (2) adding an aqueous solution of potassium chloroplatinite into the solution of step (1); adding ethylene glycol and shaking and mixing uniformly; standing to obtain the nanoparticle liquid with oxidase activity. The method disclosed by the invention has the advantages of simplicity, short cycle, mild and easy-to-control conditions and promotion of industrial mass production; with average particle size of 1.2-4.5nm, the nanoparticle liquid with oxidase activity has high catalytic activity.

Description

technical field [0001] The invention belongs to the technical field of nanoparticle preparation, and in particular relates to a platinum nanoparticle liquid with oxidase activity and a preparation method. Background technique [0002] As biocatalysts, natural enzymes have significant advantages such as high efficiency, mild action conditions, and high selectivity, and have important practical applications in the fields of medicine, chemical industry, food processing, and industry. However, since most enzymes themselves are composed of proteins, they are easily denatured and inactivated by the influence of the external environment (such as temperature, pH), and the process of preparing and purifying enzymes is complicated and costly, which greatly limits its scope of use [Inorg.Chem . Front., 2016, 3, 41-60]. [0003] The simulated enzyme system based on nanomaterials is called nanozyme. Compared with natural enzymes, nanomaterials have the advantages of low cost, high stabi...

Claims

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Application Information

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IPC IPC(8): B01J31/28B01J35/00B01J35/02
CPCB01J31/28B01J35/00B01J35/40B01J35/50
Inventor 付雁王桂倩李韡张金利黄青伟郑珊珊
Owner TIANJIN UNIV
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