A method for tissue culture and multiplication of Pseudostellaria heterophylla virus-free seedlings

Abstract

The invention discloses a tissue culturing and propagation expanding method for radix pseudostellariae toxin-free seedlings. The tissue culturing and propagation expanding method comprises the following specific steps: (1) obtaining toxin-free seedlings; (2) preparing a culture medium: weighing all the ingredients according to the following matching amounts: 7.07 g / L KNO3, 2.30 g / L NH4H2PO3, 3.4 g / L KH2PO4, 4.95 g / L MgSO4.7H2O, 4.7 g / L Ca(NO3)2.4H2O, 37.3 mg / L Na2.EDTA and 27.8 mg / L FeSO4.7H2O and adding gellan gum into the culture medium to form a solid culture medium; (3) performing tissue culture and amplification on the toxin-free seedlings; (4) propagating the toxin-free seedlings. According to the method, required nitrogen, phosphorus and potassium contents and microelements are provided for radix pseudostellariae growth. In a propagation expanding and culturing process of the radix pseudostellariae toxin-free seedlings, the culture medium is not replaced in the whole process; furthermore, no plant hormone is added, a culture process is simplified, labor cost is saved, and the tissue culturing and propagation expanding efficiency is greatly improved. A clean bench is not needed by tissue culturing and propagation expanding operation, a requirement for operators is low, simpleness and quickness in operation are achieved, the microbial pollution rate is low, the seedling survival rate is high, and production cost is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of heterophylla, in particular to a method for tissue culture and multiplication of heterophylla heterophylla virus-free seedlings. Background technique [0002] Pseudostellaria heterophylla is the root tuber of the perennial herbaceous plant False Chickweed heterophylla of Caryophyllaceae. It has the effects of benefiting Qi, invigorating the spleen, promoting body fluid and moistening the lungs. It has significant effects on children's night sweats, loss of appetite, and insomnia in the elderly. Compared with ginseng, its medicinal properties are gentler , suitable for children's tonifying deficiency and invigorating qi, therefore, it is also called children's ginseng or children's ginseng. Pseudostellaria heterophylla is a tonic Chinese medicinal material that can be used in health food. It can be used as a substitute for ginseng and even American ginseng. It has huge room for developmen...

AI Technical Summary

Problems solved by technology

However, the previously reported methods for cultivating virus-free seedlings and ginseng of Pseudostellariae Pseudostellaria all use MS (Murashige&Skoog) medium as the basic medium. This method is complex in operation, requires high technical requirements for operators, and is prone to bacterial contamination. , resulting in high cultivation costs; on the other hand, the field planting density of Pseudostellaria heterophylla is high, nearly 70,000 plants per mu, and the amount of seeds used is relatively large. The high-cost detoxified seedlings or ginseng breeding cannot meet the actual needs of agricultural production

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