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Application of CD4+CD70+T cell subset in preparing reagent for auxiliary diagnosis of very severe aplastic anemia

A technology of aplastic and cell subsets, applied in the field of biomedicine, can solve the problems such as immune-related indicators of VSAA patients that have not been reported, and achieve the effect of broad application prospects.

Active Publication Date: 2019-01-11
JINAN UNIVERSITY
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Problems solved by technology

However, the immune-related indicators of characteristic changes in VSAA patients have not been reported

Method used

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  • Application of CD4+CD70+T cell subset in preparing reagent for auxiliary diagnosis of very severe aplastic anemia
  • Application of CD4+CD70+T cell subset in preparing reagent for auxiliary diagnosis of very severe aplastic anemia
  • Application of CD4+CD70+T cell subset in preparing reagent for auxiliary diagnosis of very severe aplastic anemia

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Embodiment 1

[0049] (1) Blood was collected under the premise of signing the informed consent form with the patient, and all samples were taken from the fasting intravenous EDTA anticoagulation in the morning. A total of 23 peripheral blood samples were collected for AA, including 11 cases of VSAA and 12 cases of SAA. At the same time, 31 samples of healthy people were collected, and this part of the research protocol has been approved by the ethics committee of the unit. At the same time, the clinical data of AA patients were collected (as shown in Table 1).

[0050] Table 1

[0051]

[0052]

[0053] (2) The peripheral blood collected from healthy people and AA patients was lysed with erythrocyte lysate. Proportion 2mL red blood cell lysate per 200μL of peripheral blood, lyse at room temperature for 10min, gently blow and mix once with a pipette during lysis, then centrifuge at 200g for 5min, discard the supernatant, add 1×PBS to 2mL, at 300g Wash by centrifugation, discard the ...

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Abstract

The invention discloses an application of CD4+CD70+T cell subset in preparing reagent for auxiliary diagnosis of very severe aplastic anemia. According to the invention, the inventor finds for the first time that the proportion of CD4+CD70+T cell in the peripheral blood of AA patients is obviously reduced in A patients, especially, is most obvious in VSAA, which can be used as one of related testindices for auxiliary diagnosis of laboratory immunization of VSAA patients, and meanwhile, provides important reference data for selection of treatment strategy for VSAA patients clinically.

Description

technical field [0001] The invention belongs to the field of biomedicine, and particularly relates to the application of CD4+CD70+T cell subsets in the preparation of a kit for auxiliary diagnosis of extremely severe aplastic anemia. Background technique [0002] Aplastic anemia (AA) is a bone marrow hematopoietic failure syndrome characterized by decreased bone marrow hematopoietic cell proliferation and pancytopenia. Anemia, bleeding and infection are the main clinical manifestations of AA. The annual incidence of AA in my country is higher than that in Europe and America. The pathogenesis of AA is not yet clear, and it is currently believed that in the pathogenesis of AA, the damage of bone marrow hematopoietic function caused by abnormal T cell function is one of the reasons. Therefore, AA is also a T cell-mediated autoimmune disease. In the study of T cell immune abnormalities in AA patients, it was found that the abnormal activation of T cells played an important ro...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N33/58G01N33/564G01N15/14
CPCG01N15/14G01N33/5005G01N33/564G01N33/582G01N33/80G01N2800/22G01N2800/24
Inventor 李萡张玉平李扬秋赵素文黄桂璇王顺清肖彦恺周铭黎宇苗
Owner JINAN UNIVERSITY
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