A culture system and method for efficient differentiation of spermatogonial stem cells into neural cells

Abstract

The invention belongs to the field of cell biology, and relates to an in vitro high-efficiency culture method for differentiation of spermatogonial stem cells (SSCs) into neural cells. In the present invention, the culture vessel is treated with polylysine to increase the adherence ability of SSCs, add nerve factors B27 and N2, and remove growth factors, and the results show that SSCs can differentiate into nerve cells with high efficiency. The method established in the present invention is suitable for streamlined operation, has high repeatability, high success rate, simple operation, can achieve the effect without special conditions, and can greatly save time and test materials. In the past, pluripotent stem cells were used for neural cell induction , there is induced incompleteness. The invention uses SSCs, and compared with embryonic stem cells (ESCs), it shows the high efficiency and completeness of the differentiation of SSCs into nerve cells. The results of this study show that the induction effect of SSCs has obvious advantages. It may become a more optimized source of stem cells applied to repair nerve tissue damage and become a new technical solution.

Description

technical field [0001] The invention belongs to the technical field of cell biology research, relates to a method for differentiation of spermatogonial stem cells into nerve cells, and can be applied to the research of nerve cell differentiation and nerve tissue damage repair in clinical medical applications. Background technique [0002] At present, only rodent spermatogonial stem cells (SSCs) have been cultured for a long time in vitro, and human and other species of stem cells cannot be cultured for a long time, so mouse SSCs have become a general cell model. [0003] Spermatogonial stem cells and neural stem cells (NSCs) are closely related and have many similar stem cell characteristics. First, glial cell-derived neurotrophic factor (GDNF) is the most critical signaling factor for SSCs to maintain self-renewal, and an important factor for NSCs to maintain in vitro proliferation is brain-derived neurotrophic factor (BDNF). Cytokine (CXCL12) plays an important role in th...

AI Technical Summary

Problems solved by technology

However, the regulation mechanism of NSCs differentiation is still not thorough.

[0005] Previous studies have shown that to induce pluripotent stem cells to differentiate into nerve cells, two reagents, neural factors B27 and N2, need to be added to induce them, but there are problems such as low induction differentiation efficiency and incompleteness; in vitro, the culture system that promotes the proliferation of mouse SSCs In this method, two reagents, B27 and N2, will be added, which have an important role in promoting the proliferation of mouse SSCs; it can be seen that the in vitro culture system of SSCs is very close to the system induced by nerve cells, and SSCs may become an ideal alternative to NSCs adult stem cells

However, previous studies have not established a method for efficiently inducing spermatogonia to differentiate into nerves.

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