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Preparation method for embedded nano material/quorum quenching enzyme composite spherules

A nanomaterial, quorum sensing technology, applied in the field of functional materials and environmental applications, can solve the problems of short service life, easy biodegradation, leakage of nanomaterials, etc., to improve the recovery rate, avoid environmental pollution, improve fragile Effect

Inactive Publication Date: 2019-01-11
XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, sodium alginate is a natural polysaccharide, and when it is made into sodium alginate microspheres and used in an MBR reactor, there will be insufficient mechanical strength and easy breakage
And it is easy to be biodegraded, causing the leakage of nanomaterials, and the service life of the ball is short, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0038] A preparation method of nanomaterial / quorum sensing quenching enzyme composite bead, comprising the steps of:

[0039] 1) Preparation of nanomaterials / quorum sensing quenching enzyme particles: Disperse 25-50 mg of nanomaterials including graphene oxide, graphene and carbon nanotubes in 100-200 mL of phosphate buffer solution according to the mass ratio, and sonicate at room temperature for 30 ~ 60min to obtain a mixed solution of nanomaterials; disperse 75-150mg of quenching enzyme in the mixed solution of nanomaterials according to the mass ratio, and ultrasonically stir at 0-10°C for 30-60min to obtain a mixed solution of nanomaterials / quorum sensing quenched enzyme particles , vacuum freeze-drying to obtain nanomaterials / quorum sensing quenched enzyme particles, which are stored frozen for later use;

[0040] The above-mentioned graphene is prepared by mechanical exfoliation method, vapor deposition method (CVD), pyrolysis SiC method, graphene oxide reduction method...

Embodiment 1

[0049] Graphene oxide was prepared using Hummer's improved method. The natural flake graphite powder used is a 100 mesh powder with a purity of 99.999%.

[0050] 1) Preparation of nanomaterial / quorum sensing quenching enzyme particles: Disperse 50mg graphene oxide in 150mL 0.02MpH (6.9) phosphate buffer solution, sonicate at room temperature for 30min, add 150mg aminoacylase, and sonicate at 0°C Stir for 30 minutes, and vacuum freeze-dry to obtain graphene oxide / aminoacylase hybrid particles, which are stored in a freezer for future use.

[0051] 2) Preparation of the hydrogel mixed solution: Dissolve an appropriate amount of sodium alginate in 100ml deionized water to prepare a 0.5g / L sodium alginate solution. The mass fraction of sodium alginate obtained in step 1) is 0.1wt% nanomaterial / The quorum sensing quenching enzyme hybrid particles were added to the sodium alginate solution and ultrasonicated for 15 min; the additive chitosan with a mass fraction of sodium alginate...

Embodiment 2

[0056] The preparation of graphene adopts hydrazine hydrate reduction graphene oxide method: the graphene oxide 100mg among the embodiment 1 is dispersed in the aqueous solution 100ml, ultrasonic dispersion 50min, then add hydrazine hydrate (2ml, 50%w / v), after mixing uniformly Reaction under reflux conditions for 24h. After the reaction, the samples were filtered, separated and washed, and dried in a vacuum oven at 60°C.

[0057] 1) Preparation of nanomaterials / quorum sensing quenching enzyme particles: Disperse 25 mg of graphene in 100 mL of 0.2M pH (7.0) phosphate buffer solution, sonicate at room temperature for 40 min, add 100 mg of decarboxylase, and ultrasonically stir for 40 min at 4°C , vacuum freeze-drying to obtain graphene / decarboxylase hybrid particles, and freeze them for future use.

[0058] 2) Preparation of hydrogel mixed solution: Dissolve an appropriate amount of sodium alginate in 50ml deionized water to prepare a 2g / L sodium alginate solution, and the mas...

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Abstract

The invention discloses a preparation method for embedded nano material / quorum quenching enzyme composite spherules. The preparation method comprises the following steps: embedding embedded nano material / quorum quenching enzyme particles into a sodium alginate solution, thereby preparing sodium alginate microspheres; and coating a high polymer casting film solution added with graphene oxide, and preparing hydrogel composite spherules with organic shell and hydrogel kernel structures by use of a phase inversion method. The composite spherules are capable of inhibiting growth of a membrane-surface biological membrane by use of the bacterial signal molecule degrading function of a quenching enzyme; meanwhile, by use of the aperture, mechanical strength and antipollution characteristics of polymer shells, the erosion endurance and service lives of the composite spherules in MBR are enhanced, the spherule recovery rate is improved, and environmental pollution of nanophase materials is avoided. The embedded nano material / quorum quenching enzyme composite spherules can be widely applied to fields of biology, water treatment and the like.

Description

technical field [0001] The invention belongs to the field of functional materials and environmental applications, and relates to a preparation method for embedding nanomaterial / quorum induction quenching enzyme composite beads. Background technique [0002] Membrane bioreactor (MBR) is an efficient sewage treatment technology that combines membrane separation process and biological treatment. However, during the use of membrane materials, there are phenomena such as easy pollution and easy clogging, which affect the service life of membrane materials. Membrane fouling is considered to be the biggest obstacle restricting the wide application of MBR. The method of controlling membrane fouling includes various methods such as modification of the membrane material itself, physical and chemical cleaning, among which the modification of the membrane material itself includes improving the hydrophilic property of the membrane material, adding antibacterial nano-silver to the membra...

Claims

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Application Information

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IPC IPC(8): C08J7/04C08J3/12C08J3/075C08L5/04C08K3/04C02F3/12
CPCC02F3/1268C08J3/075C08J3/12C08J2305/04C08J2427/16C08J2481/06C08K7/00C08K3/042C08J7/0427Y02W10/10
Inventor 朱振亚王磊
Owner XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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