Method for breeding cyperus beans by cobalt 60 irradiation mutation
A technology for mutation breeding and cobalt in oleus bean, which is applied in the fields of botanical equipment and methods, plant genetic improvement, angiosperms/flowering plants, etc., to achieve the effects of increasing genetic heterogeneity, strong creativity, and shortening breeding years
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Embodiment 1
[0026] A kind of method for radiation mutagenic breeding of cobalt 60 of oily bean, comprising the steps of:
[0027] (1) Select the suitable parent of the oily bean: the oily bean must be representative, have some outstanding characters, and be suitable as the original source of provenance. Each portion of material is 1 kg, and it is sealed in a paper bag;
[0028] (2) Determination of radiation dose and time: Since cobalt-60 is a radioactive isotope and has a specific half-life, the corresponding time and dose rate at the corresponding point must be calculated according to its decay law to determine the time required for irradiation; fixed-point dose measurement, Repeat three times, and the dispersion is controlled within 5%; then determine the irradiation time according to the dose rate of the irradiation point;
[0029] (3) Seed treatment: dry the seeds before irradiation, which can not only dry the seeds, but also improve the permeability and facilitate water absorption, ...
Embodiment 2
[0037] A kind of method for the radiation mutagenic breeding of cobalt 60 of oily bean, the steps are:
[0038] (1) The parental seeds of Yousha bean have a higher oil content, which is 25.32%;
[0039] (2) After radiation mutagenesis, plant the first generation of mutagenesis (M1 seeds) in the field, select individual plants with excellent agronomic properties in the field, and determine the selected individual plants in combination with indoor oil content and other quality traits;
[0040] (3) The second generation of mutagenesis (M2 seeds) continues to be planted in the field, selected, and tested indoors to obtain seeds with high oil content, and harvest per plant according to the target traits;
[0041] (4) The harvested seeds are classified according to the target traits, and the seeds of individual plants with outstanding traits are planted in the field, and the individual plants are selected continuously according to the target traits until the target traits are stabiliz...
Embodiment 3
[0044] A kind of method for the radiation mutagenic breeding of cobalt 60 of oily bean, the steps are:
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