Cyperus esculentus breeding method and breeding equipment
A technology of oily bean and seeds, applied in the field of breeding, to achieve the effects of reasonable structure design, convenient breeding operation, and increased genetic heterogeneity
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Embodiment 1
[0022] The oily sedge bean breeding method of the present embodiment specifically comprises the steps:
[0023] (1) Parent selection: Select the representative oily bean variety seeds with excellent yield and quality as the parents, where the excellent quality means that the oil content of the seeds is above 25%;
[0024] (2) DES mutagenesis treatment: put the selected seeds into the breeding equipment of the present invention for mutagenesis treatment, that is, put the seeds into the seed placement tank 12, and loosen the bolts to move the fixing ring 9 to the bottom of the nozzle 6 , then tighten the bolt 10 so that the fixing ring 9 is fixed, pour 1% sodium hypochlorite solution in the transition zone 1.1, start the micro-water pump 3, spray 1% sodium hypochlorite on the surface of the parent seeds through the nozzle 6, and carry out surface sterilization of the parent seeds for 10 minutes , turn off the miniature water pump 3, and 1% sodium hypochlorite permeates through t...
specific example
[0029] In 2015, 3 varieties with an yield of more than 450KG per mu (dry weight of tubers) and seed oil content of 25-30% were selected from a batch of oilsha bean resources collected in Heilongjiang, Shandong, Sichuan and other places, combined with field performance and yield The results of yield measurement showed that those with relatively higher yield and higher oil content were subjected to radiation mutagenesis. Put the selected seeds into the above-mentioned breeding equipment for mutagenesis treatment (first sterilize the surface of the seeds with 1% sodium hypochlorite for 10 minutes, and then soak the seeds with 0.3% diethyl sulfate for 10-13 hours); After rinsing, it is planted in field holes, 1 seed per hole, and a single plant is selected after maturity. In 2016-2017 (M2, M3 generations), excellent lines are continuously selected in the field, and the M4 generation will be propagated in Sanya, Hainan in 2018. In 2019 By 2020, the strains with excellent M5 will be...
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