Clostridium welchii specific detection primer and detection kit

Abstract

The invention discloses a clostridium welchii specific detection primer group and a detection kit. Nucleotide sequences of the specific detection primer group are shown as SEQ ID NO:1 and SEQ ID NO:2respectively, the primer group is adopted for preparing a clostridium welchii specific detection kit, and a novel PCR (polymerase chain reaction) detection method for clostridium welchii is created. By design of special detection primers according to clostridium welchii specific sequences, the quick, specific and sensitive PCR detection method for clostridium welchii epidemiological investigationand clinical infection conditions is created, and accurate detection of clostridium welchii can be realized. The kit is simple and procedural in operation, the method is high in specificity, high sensitivity and objectiveness in result determination are achieved, epidemiological investigation of clostridium welchii, inapparent infection detection and clinical diagonisis can be realized, a promising application prospect and worthiness of large-scale popularization are achieved, and a foundation is laid for further research of clostridium welchii diagnosis, prevention and treatment techniques and the like.

Description

technical field [0001] The invention relates to the technical field of preparation of disease detection reagents, in particular to specific detection primers and a detection kit for Clostridioides welchiiosis. Background technique [0002] Clostridioides welchii is a zoonotic disease caused by Clostridium welchii. Clostridium welchii disease can cause enterotoxemia and necrotic enteritis in a variety of animals, which is closely related to the "sudden death syndrome" of livestock and poultry, and food infected with Clostridium welchii can cause food poisoning and gas gangrene in humans, causing public health security issues. Clostridium welchii disease has an acute onset, short course of disease, and rapid death. The infected animals usually die before diagnosis, which has caused great losses to the breeding industry. In addition, it is difficult to distinguish it from a variety of infectious diseases only through clinical diagnosis and pathological anatomy. Therefore, it...

AI Technical Summary

Problems solved by technology

[0003] For a long time, the most classic and accurate method to detect Clostridium welchii is the toxin neutralization test, but it has the disadvantage of misjudgment caused by non-specific death, and it is time-consuming and laborious, and the test period is long

At present, most of the identification of bacteria still use traditional bacteriological methods and animal experiments, but there are disadvantages such as cumbersome steps, long cycle, low detection rate, and the accuracy of the experimental results cannot be guaranteed.

Other diagnostic methods, such as ELISA detection method, are mainly used for serotype typing, and are rarely used in laboratory diagnosis and clinical diagnosis; although the detection of antitoxin has good sensitivity and specificity, the process of preparing antitoxin is very cumbersome. And it needs to be purified to detect the toxin antigen, and it is prone to non-specific reactions, so this method is not commonly used; the reported detection method is basically based on the α-toxin gene, but other bacteria, such as Clostridium putrefaction, also have α -The toxin gene may also be amplified, which will affect the detection results, so the use of α-toxin gene-specific PCR has limitations

[0004] In summary, although these identification methods are still used in the diagnosis of Clostridioides welchiisis so far, there is an urgent need for more accurate, rapid and easy-to-operate detection methods in clinical practice.

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